Fuels, diesel

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1995

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Fuels, diesel

EC Number:

269-822-7

EC Name:

Fuels, diesel

Cas Number:

68334-30-5

IUPAC Name:

1-(heptan-2-yl)-4-hexyl-2-(3-methylhexyl)cyclopentane; 1-butyl-2-(nonan-3-yl)-4-propylbenzene; 2,3,7-trimethyl-6-(propan-2-yl)nonane; triacontane

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

not specified

Water media type:

not specified

Total exposure duration:

72 h

Remarks on exposure duration:

EL50 values determined by probit analysis. Williams test used to determine NOELs.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

ca. 25 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

biomass

Remarks on result:

other: 95% confidence interval of 21 - 30 mg/l

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

ca. 78 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

growth rate

Remarks on result:

other: 95% confidence interval of 64 - 96 mg/l

Any other information on results incl. tables

Based on nominal loading rates:

72-hr EL50 (biomass) = 25 mg/l 95% confidence interval of 21 - 30 mg/l

72-hr EL50 (growth rate) = 78 mg/l 95% confidence interval of 64 - 96 mg/l

72-hr NOEL (biomass) = 3 mg/l

72-hr NOEL (growth rate) = 10 mg/l.

Nominal	72h	72 h Mean Cell conc.
Conc.(mg/l)	% Inhibition	(million cells/ml)
Control	n/a	0.14
1.0	-21	0.17
3.0	-36	0.19
10	29	0.10
30	61	0.055
100	91	0.013
300	94	0.0087
1000	95	0.0074

n/a - Not applicable

The pH increased by more than one unit (1.1 units) during the test as a result of good culture growth and could not be avoided. Temperature range in the incubator was outside the preferred range of 21 - 25 °C. The out-of-range readings were recorded in the first four hours of the test probably due to temperature fluctuations associated with opening and closing the incubator. These deviations were not sufficient to invalidate the study. Analytical method used was gas chromatography-mass spectrometry. Mean reduction in the concentration of dissolved hydrocarbons during the test was 14% (range 0 - 40%).