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EC number: 223-051-2 | CAS number: 3709-43-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Referenceopen allclose all
- Reference Type:
- other: Authoritative data base
- Title:
- GCID 221778
- Year:
- 2 011
- Bibliographic source:
- ACToR (Aggregated Computational Toxicology Resource);Ciba-Geigy Limited (1986). Final Report FAT 90159/A. Acute eye irritation/corrosion study in the rabbit. Experimental Toxicology GU 2.1, Project No. 851037 (unpublished study)
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Principles of method if other than guideline:
- ACTOR
Data is from U.S. Environmental Protection Agency Hazard Characterization Document - GLP compliance:
- not specified
Test material
- Reference substance name:
- Disodium 4,4'-dinitrostilbene-2,2'-disulphonate
- EC Number:
- 223-051-2
- EC Name:
- Disodium 4,4'-dinitrostilbene-2,2'-disulphonate
- Cas Number:
- 3709-43-1
- Molecular formula:
- C14H10N2O10S2.2Na
- IUPAC Name:
- disodium 4,4'-dinitrostilbene-2,2'-disulphonate
- Details on test material:
- - Name of test material : disodium 4,4'-dinitrostilbene-2,2'-disulphonate
- Substance type: Organic
- Physical state: Solid
purity : (60% test substance; 35% water)
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
Test system
- Vehicle:
- water
- Controls:
- yes
- Duration of treatment / exposure:
- 24 – 72 hour
- Observation period (in vivo):
- 7 days
- Number of animals or in vitro replicates:
- 3 males
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- conjunctivae score
- Remarks:
- conjustival redness score
- Basis:
- mean
- Score:
- 1
- Remarks on result:
- other: Conjunctival redness scores of 1 (some blood vessels definitely hyperaemic) were observed up to 72 hours in one rabbit.
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Score:
- 1 - 2
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Score:
- 1
- Remarks on result:
- other: Chemosis scores of 1 (any swelling above normal, including nictitating membrane) were observed at 1 and 24 hours in 2 animals and at 1 hour in the third animal.
Any other information on results incl. tables
Conjunctival redness scores of 1 (some blood vessels definitely hyperaemic) were observed up to 72 hours in one rabbit. In another rabbit, conjunctival scores of 2 (diffuse, crimson color, with individual vessels not easily discernable) were observed after 1 and 24 hours, and a score of 1 was observed at 48 hours. In the third rabbit, the conjunctival score at 1 hour was 2, followed by 1 at 24, 48 and 72 hours.
Chemosis scores of 1 (any swelling above normal, including nictitating membrane) were observed at 1 and 24 hours in 2 animals and at 1 hour in the third animal. All other scores were 0. The average scores for redness over the 24-72 period were 1 in all of the animals. The average scores for chemosis over the 24 - 72 period were 0.33, 0.33 and 0 in the three animals.
According to the EC classification, the results from 24 to 72 hours were classified as non-irritant (redness and chemosis scores >= 2.5 or 2, respectively were not obtained). The eye reactions were reversible until the end of the observation period on day 7. Slight weight loss was observed in two of the animals on day 3. Weights of all the animals on day 7 were greater than initial weights.
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- disodium 4,4'-dinitrostilbene-2,2'-disulphonate was not irritating to rabbit eyes in this assay.
- Executive summary:
New Zealand White rabbits (3 males) received an ocular administration of benzenesulfonic acid, 2,2’-(1,2-ethenediyl)bis[5-amino-, disodium salt] disodium 4,4’-dinitrostilbene-2,2’-disulphonate (60% test substance; 35% water) at 0.1 mL and observed for 7 days following treatment. The treated eye was not washed; the untreated eye served as control. The average scores for redness over the 24 – 72 hour period were one in all of the animals. The average scores for chemosis over the 24 – 72 hour period were 0.33, 0.33 and 0 in the three animals. Benzenesulfonic acid, 2,2’-(1,2-ethenediyl)bis[5-amino-, disodium salt] disodium 4,4’-dinitrostilbene-2,2’-disulphonate was not irritating to rabbit eyes in this assay.
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