4,5,6,7-tetrahydrothieno[3,2-c]pyridinium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 26 february 1996 to 9 august 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: A well documented GLP study according to european guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4,5,6,7-tetrahydrothieno[3,2-c]pyridine hydrochloride

IUPAC Name:

4,5,6,7-tetrahydrothieno[3,2-c]pyridine hydrochloride

Constituent 2

Reference substance name:

4,5,6,7-tetrahydrothieno[3,2-c]pyridinium chloride

EC Number:

249-220-0

EC Name:

4,5,6,7-tetrahydrothieno[3,2-c]pyridinium chloride

Cas Number:

28783-41-7

Molecular formula:

C7H9NS.ClH

IUPAC Name:

4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-ium chloride

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in the study report): chlorhydrate de 4, 5, 6, 7-tétrahydrothiéno [3, 2-c] pyridine de code PCR 0665
- Substance type: organic
- Physical state: white powder
- Analytical purity: 99.4%
- Lot/batch No.: 4 SNS 011
- Solubility: soluble in water
- Stability: stable under normal conditions of use
- Decomposition: around 244°C
- Storage condition of test material: store the container closed and in well ventilated area

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

As the test substance hydrochloride, 4, 5, 6, 7-tetrahydrothieno [3,2-c] pyridine code PCR 0665 is soluble in water, a stock solution of 1 g/l is prepared by dissolving the analyte in the test medium. After stirring for 2 hours at 20 °C, the solution was filtered to 0.22 µm to remove any suspended particles. This stock solution is then diluted in the test medium to obtain the appropriate test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Daphnies: Daphnia magna Straus 1820 (Cladocera crustacea)
- Source: INERIS laboratory
- Method of breeding: continuous and balanced breeding in accordance with Appendix A of the standard AFNOR NF T 90 301.
- Feeding: TetraMin + algae
- Illumination: daylight + artificial light with a photoperiod of 16 hours light and 8 hours of darkness.

Study design

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

in darkness

Test conditions

Test temperature:

20°C +/- 2°C

pH:

Filtered stock solution: 7.24
Test solution at the highest tested concentration: 7.30

Dissolved oxygen:

Dissolved oxygen content measured at the end of the end of the test (after 48h) is higher than 2 mg/l for the test substance.

Nominal and measured concentrations:

Measured concentrations: 36 - 51 - 72 - 100 - 145 - 197 - 288 and 394 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tubes for single use
- Fill volume: sufficient capacity for 10 ml
- Aeration: none
- No. of organisms per vessel: 5 daphnies per vessel (2 ml for each daphnie)
- No. of vessels per concentration (replicates): 4 vessels for each concentration tested
- No. of vessels per control: 4 vessels each containing 10 ml of test medium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4

TEST CONCENTRATIONS
- Test concentrations: 36 - 51 - 72 - 100 - 145 - 197 - 288 and 394 mg/l

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

141 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 119-169 mg/l

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

72 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 61-83 mg/l

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

< 36 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

288 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

EC50-48h determined by the method of calculation of PROBITS (confidential limits 95%): 141 mg/l (119-169 mg/l).
EC50-24h determined by the method of calculation of PROBITS (confidential limits 95%): 72 mg/l (61-83 mg/l).

Results with reference substance (positive control):

EC50-24h of potassium dichromate is 1.17 mg/l and is therefore in the range recommended by the AFNOR NF-T-90 301 (0.9-1.5 mg/l).

Reported statistics and error estimates:

In the test conditions, the percentage loss of the test substance remained after 48 hours less than 20% for all test concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In an acute toxicity test with daphnia the EC50 of the test substance was found 141 mg/l at 48 h and 72 mg/l at 24h. Thus the substance is not considered toxic to Daphnia magma.