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EC number: 257-036-7 | CAS number: 51181-50-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
72h EC50 >72.5 mg/l, 72h NOEC 58.5 mg/l for P. subcapitata as HEDTA-FeNa.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 58.5 mg/L
Additional information
1. A study was carried out with Pseudokirchneriella subcapitata (formerly known as Selenastrum capricornutum) (Geurts and Van Wijk 2001, Rel. 1, Key study). The guidelines were slightly modified to ensure good growth and pH control of the cultures. The test was carried out on equimolar amounts of NaHEDTA.2H20 and FeCl3.6H20 and at nominal concentrations of 60, 80 and 100 mg/l (concentrations represented as H4.EDTA). Due to photodegradation, the test substance concentration declines during the test. The mean concentrations during the 72 hours of testing for the nominal concentrations of 60, 80 and 100 mg/l as measured by chemical analyses were 38.1, 48.4 and 60.6 mg/l, respectively. The EbC50and ErC50 are both higher than 100 mg/l based on nominal concentrations and higher than 60 mg/l based on the mean measured concentrations of H4.EDTA. Recalculated to HEDTA-FeNa the 72h-EC50s are above 72.5 mg/l.
The NOEC and LOEC based on nominal concentrations are 79.4 mg/l and 99.9 mg/l (H4-EDTA). Based on the mean measured concentrations the NOEC and LOEC are 48.4 and 60.6 mg/l (H4-EDTA). Recalculated to HEDTA-FeNa, the 72h-NOEC = 58.5 mg/l.
2. A guideline study with DTPA-FeNaH (Kean, 2013) with P. subcapitata and including attempts to address for the chelating properties as well as colour of the test solution. This substance caused coloration of the test solutions and proved photolabile. A significant decline of most of the test concentrations was evident, but observed unexpected results wrt measured concentrations were not explained. A NOEC > 1 mg/L was confirmed by measured and nominal concentrations indicating little concern for the aquatic environment, however as there is some unclarity wrt geometric mean measured concentrations, these results were not considered key.
3. Additional confirmation of the toxicity of chelants, associoated with the scavenging of essential micronutrients was provided in the two additional studies. First, the illustration by Dufkova (1984) that no negative influence to algae growth was observed when the concentration of nutrients in solution was increased, corresponding to the increased EDTA concentration. In addition, the European Union Risk Assessment Report Edetic Acid (EDTA) of 2004 reviews a study on the acute toxicity of equimolar amounts of Na4 -EDTA and Fe(III) towards Scenedesmus subspicatus. No inhibition was found up to a nominal concentration of 100 mg Na4EDTA.
The most relevant and concrete data wrt toxicity to algae is considered the first study by Geurts & van Wijk and these endpoints are considered to be relevant for HEDTA-FeNa, based on the read-across justification as provided in section 13.
The chelation of essential nutrients is not considered a relevant environmental hazard, due to the abundance of these nutrients in the aquatic environment.
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