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EC number: 446-560-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Mar - 06 Apr 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.06, 0.13, 0.25, 0.5 and 1 mg/L
- Sampling method: Sampling schedule of chemical analysis 0 and 48 h - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared to give series of test concentrations. To achieve this 2.4 mg of the test substance were added to 2 litres of dilution water, treated with an ultra turrax for 60 seconds and afterwards stirred for 24 h on a magnetic stirrer. Finally undissolved particles of the test substance were removed by filtration.
- Controls: 1 control
- Test concentration separation factor: 2
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None after filtration - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): Neonates
- Stage and instar at study initiation: Neonates
- Method of breeding: well-established test culture
- Source: Strain of Bundesgesundheitsamt Berlin
- Feeding during test: No
ACCLIMATION
- Acclimation conditions (same as test or not): The culture water (so-called 'M4 medium') is partly renewed once a week.
- Type and amount of food: The Daphnia are exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'.
- Feeding frequency: daily
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS: The neonates are separated from their parent Daphnia by filtration prior to the acute test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 15.8 °dH (= 282 mg/L CaCO3)
- Test temperature:
- 20.0 °C
- pH:
- 7.7 - 7.8
- Dissolved oxygen:
- 8.8. - 9.1 mg O2/L
- Nominal and measured concentrations:
- 0.06, 0.13,0.25, 0.5 and 1 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Karlsruher Flaschen holding 10 neonates in 250-300 mL of test medium
- Type (delete if not applicable): closed
- Volume of solution: 250-300 mL
- Aeration: No aeration
- No. of organisms per vessel: 10 neonates per vessel
- No. of vessels per concentration (replicates): 2 replicates per concentration
- No. of vessels per control (replicates): 2 replicates per control
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (M4-medium)
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16:8 light:dark
- Light intensity: < 1000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation after 24 and 48 h - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 0.97 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 0.24 - <= 0.97 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.24 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Conclusions:
- EC50 (48 h): >0.24 - <0.97 mg/L (nominal)
- Executive summary:
The Daphnia were exposed to a range of concentrations, nominally 0.06, 0.13, 0.25, 0.5 and 1 mg/L of the test substance dissolved in water. Auxiliaries used to prepare the testmedia were an ultras turrax, a magnetic stirrer and a folded filter. Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined: EC0 = 0.24 mg/L, EC50: >0.24 - <0.97 mg/L, EC100 = 0.97 mg/L (48 h).
The results are expressed in terms of nominal concentrations (at 24 h), and in terms of mean measured concentrations (at 48 h). Measured concentrations ranged from 90 - 96.6% of nominal values at 0 hours, and from 91.7 - 97.6% of nominal values at 48 hours, respectively.
Reference
Validity criteria for the measurement of the short-term toxicity to aquatic invertebrates:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
Young daphnids, aged less than 24 hours at the start of the test, are exposed to the test substance at a range of concentrations for a period of 48 h. | Neonates were used in this test. | Yes |
In the control, including the solubilising agent, not more than 10 % of the daphnids should have been immobilized. (Not more than 10 % of the control daphnids should show immobilisation or other signs of disease or stress, for example, discoloration or unusual behaviour such as trapping at surface of water) | In the control 0% mortaility an no other adverse effects were observed. | Yes |
At least five test concentrations should be used. They should be arranged in a geometric series with a separation factor preferably not exceeding 2.2. The highest concentration tested should preferably result in 100 % immobilization, and the lowest concentration tested should preferably give no observable effect. | Five test concentrations were used. EC0 and EC100 were determined. The test concentration separation factor was 2. | Yes |
Description of key information
The Daphnia were exposed to a range of concentrations, nominally 0.06, 0.13, 0.25, 0.5 and 1 mg/L of the test substance dissolved in water. Auxiliaries used to prepare the testmedia were an ultras turrax, a magnetic stirrer and a folded filter. Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined: EC0 = 0.24 mg/L, EC50: >0.24 - <0.97 mg/L, EC100 = 0.97 mg/L (48 h).
The results are expressed in terms of nominal concentrations (at 24 h), and in terms of mean measured concentrations (at 48 h). Measured concentrations ranged from 90 - 96.6% of nominal values at 0 hours, and from 91.7 - 97.6% of nominal values at 48 hours, respectively.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 0.24 - < 0.97 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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