Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 406-250-0 | CAS number: 72619-32-0 HALOXYFOP R-(+)-ME HERBICIDAL CHEMICAL
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Haloxyfop-R methyl ester (XRD-535)
Lot #: RMM 2055
Purity: 96.4% - Analytical monitoring:
- yes
- Details on sampling:
- Samples for analysis of the test media were taken on nine occasions during the 28-day exposure duration. On each sampling occasion samples for analysis were taken from both aqueous stock solutions, both the control test media and test media containing test substance, except day 16 when only the two controls and test media containing test substance were analysed.
- Vehicle:
- yes
- Remarks:
- Acetone
- Details on test solutions:
- Two solvent stock solutions were prepared by addition of test substance to acetone. The nominal concentrations of the solvent stock solutions were 25 and 0.88 mg/mL. These solvent stock solutions, prepared every seven days of the test and stored in a fridge, were used to prepare two aqueous stock solutions by direct addition to dilution water. The aqueous stock solutions were prepared daily for the duration of the test. The nominal concentrations of the aqueous stock solutions were 2.0 and 0.07 mg/L. The aqueous stock solutions were diluted with water to produce the following nominal concentrations of test substance: 0.7, 0.2, 0.07, 0.02, 0.007 and 0.002 mg/L.
For the solvent control treatment a solvent stock solution was prepared daily by direct addition of 0.8 mL acetone to 10 L dilution water. The stock solution was diluted with water. A further control treatment was prepared with dilution water only. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Strain: Oncorhynchus mykiss
- Source: Fish Network (Upper Hayne Farm, Blackborough, UK),
- Length at study initiation: 6.1 cm (range 6.0-6.5 cm)
- Weight at study initiation: 3.0 g (range 2.5-3.7 g)
Feeding during test
- Food type: Hatchery 800 (BOCM Pauls)
- Amount: At a rate of 2% fresh body weight
- Frequency: Single feed
ACCLIMATION
- Acclimation period: More than 14 days - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
- Hardness:
- 52.9-56.5 CaCO3 mg/L
- Test temperature:
- 14.5-15.1°C
- pH:
- 6.8-7.4
- Dissolved oxygen:
- 61-100% air saturation
- Nominal and measured concentrations:
- Nominal: 0 (water control), 0 (solvent control), 0.002, 0.007, 0.02, 0.07, 0.2, 0.7 mg/L
Measured:- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria
- Size of vessel: 14 L
- Type: Closed
- Material, size, headspace, fill volume: 14 L glass aquaria containing 10 L of test medium
- Aeration: The test vessels were not aerated
- Type of flow-through: Stock solutions and dilution water were delivered continuously to the test vessels using an automated, piston action, syringe dosing apparatus (ADA). The solvent control and control test media were delivered using peristaltic pumps.
- Renewal rate of test solution (frequency/flow rate): 7 complete test medium changes per vessel each day. Flow rate: 50 mL/min
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- No. of vessels per vehicle: 1
- Biomass loading rate: 0.43 g body weight/L (volume in 24 hours)
TEST MEDIUM / WATER PARAMETERS
- Chlorine: 0.01-0.05 mg/L
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light: 8 hours dark- Reference substance (positive control):
- no
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.128 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: ester equivalents
- Basis for effect:
- mortality
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.005 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: ester equivalents
- Basis for effect:
- other: sub-lethal symptoms
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.447 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: ester equivalents
- Basis for effect:
- weight
- Remarks on result:
- other: highest concentration tested
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.043 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: ester equivalents
- Basis for effect:
- length
- Duration:
- 28 d
- Dose descriptor:
- LC50
- Effect conc.:
- 0.237 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: ester equivalents
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.447 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: ester equivalents
- Basis for effect:
- mortality
- Remarks on result:
- other: highest concentration tested
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.447 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: ester equivalents
- Basis for effect:
- mortality
- Remarks on result:
- other: highest concentration tested
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.447 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: ester equivalents
- Basis for effect:
- mortality
- Remarks on result:
- other: highest concentration tested
- Duration:
- 4 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.447 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: ester equivalents
- Basis for effect:
- mortality
- Remarks on result:
- other: highest concentration tested
- Duration:
- 1 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.447 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: ester equivalents
- Basis for effect:
- mortality
- Remarks on result:
- other: highest concentration tested
- Details on results:
- - Observed effects at each concentration for each observation time: Mild sub-lethal effects were observed in one fish, on day 21 only in fish exposed to 0.0019 mg/L, by day 22 and subsequently no effects were seen. Mild sub-lethal effects were observed in fish exposed to 0.0135 mg/L from day 4 until day 14, and were seen in fish exposed to 0.0427 mg/L from day 3 and 0.1281 mg/L from day 2 until the end of the test. Mild effects included slightly darkened colouration and apparent loss of appetite and increased 'coughing' frequency. Between two and four of the fish exposed to 0.1281 mg/L exhibited severe toxic effects between days four and ten, some of the remaining fish exhibited mild effects from day 2 and from day 13 all of the fish exhibited mild symptoms until the end of the test.
- Concentrations that produce lethal or other effects: Mild and severe toxic effects were observed at 0.4470 mg/L from day 1, and from day 20 onwards all fish were severely affected until the end of the test. The severe effects demonstrated by the fish at 0.4470 mg/L included loss of appetite and lethargy on day 3, and occasional loss of swimming control on day 4. By day 16 these fish displayed darker colouration than controls and an increased 'coughing' frequency was evident until the end of the test.
- Cumulative mortality at each concentration and for each recommended observation time if possible: On day 4, 10% mortality was observed at the highest concentration of 0.4470 mg/L, increasing to 20% on day 21 and finally 100% by day 28. No mortalities were observed at any other test concentrations.- Reported statistics and error estimates:
- The 28-day LC50 value was calculated using probit analysis after log10 transformation. Fresh weight and fork lengths were analysed using analysis of variance and Dunnets test.
- Validity criteria fulfilled:
- yes
- Conclusions:
- 28-day NOEC (Rainbow trout): 0.1281 mg/L (Mortality)
28-day NOEC (Rainbow trout): 0.0052 mg/L (Sub-lethal symptoms)- Executive summary:
The prolonged toxicity of the test substance to Oncorhynchus mykiss was determined in a 28-day flow-through test with continuous renewal of the test media. The study was carried out in accordance with OECD guideline 204.
Test media were prepared at the following nominal concentrations: 0.7, 0.2, 0.07, 0.02, 0.007 and 0.002 mg/L. Both a solvent control (dilution water and solvent at the same concentration as delivered to the test substance treated vessels, i.e., 0.08 mL per L) and a control (dilution water only) treatment were used. There was one test vessel per treatment containing ten fish, at the start of the exposure phase.
Stock solutions and dilution water were delivered continuously to the test vessels using an automated, piston action, syringe dosing apparatus (ADA). The solvent control and control test media were delivered using peristaltic pumps.
Samples for analysis of the ester and acid hydrolysis product of the test substance were taken on nine occasions during the 28-day exposure duration. Mean measured concentrations were based on the sum of the acid, converted to ester equivalents and ester measured concentrations.
A summary of the mean measured concentrations in the test media (ester plus acid as ester equivalents) are: 0.0019, 0.0052, 0.0135, 0.0427, 0.1281, 0.4470 mg/L.
The toxicity of test substance to O. mykiss was expressed in terms of the mean measured concentrations (sum of acid, converted to ester equivalents and ester components) in the test media.
Mild toxic effects were observed at 0.0135 and 0.0427 mg/L and both mild and severe effects were observed at 0.1281 (transitory severe effects) and 0.4470 mg/L. Mortalities were observed at the highest concentration, 0.4470 mg/L, resulting in 10% mortality after four days and 100% mortality by the end of the 28-day exposure period.
The 28-day LC50 value was 0.2369 mg/L
There were no significant differences between treatments for mean wet weight compared to the solvent control. There were significant differences (P<0.01) between the 0.1281 and 0.4470 mg/L treatments and the solvent control for mean fork length.
Based on mortality, the highest no effect concentration (NOEC) was observed to be 0.1281 mg/L. Based on sub-lethal symptoms, the NOEC was observed to be 0.0052 mg/L. Based on wet weights and forks lengths of surviving fish the NOEC's were calculated to be > 0.4470 mg/L for weight, the highest concentration tested and 0.0427 mg/L for length respectively. Overall, the NOEC is considered to be 0.0052 mg/L.
Reference
Description of key information
28-day NOEC (Oncorhynchus mykiss, Rainbow trout): 0.0052 mg/L (Sub-lethal symptoms); OECD 204, Reliability = 1
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.005 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.