Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 701-270-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15th-17th January, 2014
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- GLP not required at time of test initiation
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 3M Maplewood, Lot# 20125
- Expiration date of the lot/batch: >1 year after manufacture date (29 Oct 2013)
- Purity test date: 14th January, 2014
- Appearence: Liquid, brown-green
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Stored at room temperature (15 to 30 °C)
- Stability under test conditions: Stable at room temperature. Can oxidize in presence of oxygen at high temperatures. - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Water accommodated fractions were prepared by adding 0, 0.00035, 0.0007, 0.0014, 0.0028, and 0.0056 g of the test substance to 3.5L of dilution water to create a blank control, and nominal concentration rates of 0.1, 0.2, 0.4, 0.8 and 1.6 mg/L. Each WAF concentration was stirred for 48 hours (gradual increase to 250 rpm), after which the solution was allowed to settle for 1 hour. The vessels containing the solution were wrapped in foil and covered with plexiglas lids during the stirring. The solutions were then siphoned from the center of the water column, with the first ~100 mL discarded.
- Controls: Laboratory blank and WAF control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): All solutions were clear and colorless after siphoning. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: In house culture. Original stock from Aquatic BioSystems (Ft. Collins CO)
- Feeding during test: No, neonates fed ca. 1.0 mL of P. subcapitata before test
ACCLIMATION:
- Acclimation period: None, organisms were <24 hours old
- Rearing conditions: Same as test
- Food type: Pseudokirchineriella subcapitata and yeast-cereal-grass fermented-trout-chow (YCT).
- Amount: 2.5 mL each of P. subcapitata and YCT.
- Frequency: 3 times weekly.
- Health during acclimation of parental stocks (any mortality observed): 1.6% mortality in 7 days preceding test.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 92 mg/L CaCO3
- Test temperature:
- 18.7 - 20.2 °C
- pH:
- 7.9 - 8.2
- Dissolved oxygen:
- 9.0 - 9.4 mg/L
- Conductivity:
- 321-336 uS/cm
- Nominal and measured concentrations:
- Concentrations: Nominal- Lab water blank, WAF blank, 0.1, 0.2, 0.4, 0.8, 1.6 mg/L (prepared as Water Accomodated fractions)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass beakers covered with Plexiglas
- Test solution volume: 200 mL
- Aeration: None. Test water was vigorously aerated with filtered oil-free compressed air passed through a glass Pasteur pipette before use.
- No. of organisms per vessel: Five
- No. of vessels per concentration (replicates): Four
- No. of vessels per control (replicates): Four
- No. of vessels per procedural control (replicates): Four
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Water with additional of: MgSo4 (0.0572 g/L), CaSO4*H2O (0.0835 g/L), NaHCO3 (0.1056 g/L), KCl (0.0044 g/L), Vitamin B12 (2 ug/L), Selenium (2 ug/L).
- Ca/mg ratio: 1.0
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 16h light:8h dark, 30 minute transition period
- Light intensity: 590-618 lux, full spectrum
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observations for general behavior (immobilization) were measured at test initiation, 24 and 48 hours during the test.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2-fold
- Range finding test: Yes
- Test concentrations: 0, 0.1, 1, 10, 100 Test Item/L.
- Results used to determine the conditions for the definitive study: 0 daphnia dead at 0.1 mg/L, total mortality at 1.0 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Zinc Sulfate Heptahydrate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 0.57 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other:
- Remarks:
- 95% CI: 0.32, 0.96 mg/L
- Details on results:
- - Behavioural abnormalities: No
- Other biological observations: No
- Mortality of control: No
- Other adverse effects control: No
- Abnormal responses: No
- Effect concentrations exceeding solubility of substance in test medium: No. Solutions appeared clear and colorless at beginning and end of test - Results with reference substance (positive control):
- - Results with reference substance valid? yes
48 hour LC50=0.58 mg Zn2+/L
- Other: refernce substance test initiated one day prior to definitive test - Reported statistics and error estimates:
- Statistical evaluations were performed on immobilization of Daphnia magna using a Binomial method. The values were calculated using the CETIS statistical package (Version 1.7.0.3).
- Validity criteria fulfilled:
- yes
- Remarks:
- <10% mortality in controls (0%), DO >3 mg/L (9 mg/L)
- Conclusions:
- 48 hour EL50 of 0.57 mg/L (OECD 202) in Daphnia magna for immobilization.
- Executive summary:
The 48 hour EL50 of MTDID 18990 to Daphnia magna was determined according to OECD 202 guidelines. Nominal concentrations of 0.1, 0.2, 0.4, 0.8, and 1.6 mg/L (prepared as Water Accommodated Fraction), a laboratory control, and a WAF control were run using 5 animals per vessel, with four replicates per concentration. An EL50 of 0.57 mg/L was determined.
The study was well-documented, followed an international standard method, but was not GLP compliant and had no analytical monitoring of test substance. The study is considered reliable with restrictions. The results from this study are considered suitable for Risk Assessment, Classification and Labeling, and PBT Analysis.
Reference
Table 1. Daphnia Magna Immobilization in 48 hr Exposure
Nominal loading rate (mg/L) |
Mean ± SD (%) |
Replicate A (# / %) |
Replicate B (# / %) |
Replicate C (# / %) |
Replicate D(# / %)
|
Lab Control |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
WAF control |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0.1 |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0.2 |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0.4 |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0.8 |
100 ± 0 |
5 / 100 |
5 / 100 |
5 / 100 |
5 / 100 |
1.6 |
100 ± 0 |
5 / 100 |
5 / 100 |
5 / 100 |
5 / 100 |
Description of key information
48 hour EL50 of 0.57 mg/L (OECD 202) in Daphnia magna for immobilization.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.57 mg/L
Additional information
The 48 hour EL50 of MTDID 18990 to Daphnia magna was determined according to OECD 202 guidelines. Nominal concentrations of 0.1, 0.2, 0.4, 0.8, and 1.6 mg/L (prepared as Water Accommodated Fraction), a laboratory control, and a WAF control were run using 5 animals per vessel, with four replicates per concentration. An EL50 of 0.57 mg/L was determined.
The study was well-documented, followed an international standard method, but was not GLP compliant and had no analytical monitoring of test substance. The study is considered reliable with restrictions. The results from this study are considered suitable for Risk Assessment, Classification and Labeling, and PBT Analysis.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.