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EC number: 423-860-2 | CAS number: 56309-94-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-12-18 till 1998-01-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Version / remarks:
- December 1992
- Deviations:
- no
- GLP compliance:
- yes
- Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Pretest: The concentration of the test substance was determined immediately after preparation (t=0), after 2.4 h and after 5 days.
Test 1: The concentration of the test substance was determined immediately after preparation (t=0) and 1, 2, 3, 3.5, 4, 4.5, 5, 5.5 and 6 hours after t=0.
Test 3: The concentration of the test substance was determined immediately after preparation (t=0), after 16 and 19 h after t=0. - Buffers:
- - pH: 4
sterile 0.05 M acetat buffer (sodium acetate/ acetatic acid/ Milli-Q water)
- pH: 7
sterile 0.05 M phosphate buffer (potassium dihydrogenphosphate/ sodium hydroxide/ Milli-Q water)
- pH: 9
sterile 0.05 M borate buffer (boric acid/ potassium chloride/ sodium hydroxide/ Milli-Q water) - Details on test conditions:
- Preparation of the test solutions
Prior to the performance of each test, the test solutions were freshly prepared. Therefore, an accurately weighed amount of approximately 23-32 mg test substance was added to 50.0 mL buffer solution pH 4, pH 7 or pH 9. After sonication for approximately 15 minutes, the resultant solutions were filter-sterilized through a 0.2 µm membrane filter (FP 030/3, Schleicher & Schuell, 's-Hertogenbosch, The Netherlands) and transferred into sterile glass vessels. To exclude oxygen, nitrogen gas was bubbled through each solution for approximately 5 minutes. Thereafter, each vessel was tightly sealed with a septum crimp cap.
Preliminary test
After preparation, the test solutions at pH 4, pH 7 and pH 9 were placed in a thermostatically controlled water bath at 50.0 ± 0.6°C in the dark. The concentration of the test substance was determined immediately after preparation (t=0), after 2.4 hours and after 5 days.
Testing of pseudo-first order behaviour (Test 1 according to EEC guideline C.7)
During the preliminary test, significant hydrolysis (i.e. a decrease in test substance concentration at 50°C < 50% after 2.4 hours but > 10% after 5 days) was observed at pH 4. Therefore, Test 1 was carried out at this pH value. After preparation, the test solution at pH 4 was placed in a thermostatically controlled water bath at 40.7 ± 0 1°C in the dark. The concentration of the test substance at pH 4 was determined immediately after preparation (t=0) and 1, 2, 3, 3.5, 4, 4.5, 5, 5.5 and 6 hours after t=0. For each test solution, the logarithms of the relative concentrations between 20% and 70% hydrolysis were plotted against time. When this is a straight line, the reaction is considered to be (pseudo)-first order and Test 3 according to EEC guideline C.7 must be performed. The results from Test 1 for pH 4 were also used for the calculation of k(25°C) in Test 3. For that purpose, the logarithms of all relative concentrations were plotted against time.
Determination of the half life time (t1/2) at 25°C (Test 3 according to EEC guideline C.7)
Because the resultant line from Test 1 was a straight line for pH 4 Test 3 was carried out. This test was performed at 30.5°C. After preparation, the test solution was placed in a thermostatically controlled water bath at the specified temperature, and in the dark. For pH 4 and 30.5°C, the concentration of the test substance was determined immediately after preparation (t=0) and 16 and 19 hours after t=0. The logarithms of all relative concentrations were plotted against time for these pH values. The results from Test 1 for pH 4 at 40.7°C were also used.
pH measurements
For each test solution the pH value at room temperature was determined at the beginning and at the end of each test. - Duration:
- 5 d
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- 0.359 g/L
- Duration:
- 5 d
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- 0.479 g/L
- Duration:
- 5 d
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- 0.365 g/L
- Number of replicates:
- Preliminary test, Test 1 and Test 3: duplicate analysis
- Positive controls:
- not specified
- Negative controls:
- not specified
- Preliminary study:
- At pH 7 and pH 9, a decrease in concentration < 10% was observed after 5 days (half-life time at 25°C > 1 year). Therefore, no further testing was necessary at pH 7 and pH 9. At pH 4, a decrease in concentration < 50% after 2.4 hours but > 10% after 5 days was observed. In order to determine if hydrolysis of the test item is a pseudo-first order reaction, a subsequent test was performed at pH 4.
- Test performance:
- Test 1:
For the test solution pH 4, the plot of the logarithms of the relative concentrations between 20% and 70% hydrolysis against time is a straight line. Therefore, the reaction at pH 4 is considered to be (pseudo) first order. Hence, the half-life time at 25 °C was determined at this pH value in a subsequent test. The results of Test 1 are also used.
Test 3:
From each regression line, the rate constants and the corresponding half-life time at 25 °C were calculated. - Transformation products:
- not specified
- % Recovery:
- 0
- pH:
- 4
- Temp.:
- 50 °C
- Duration:
- 5 d
- % Recovery:
- 104
- pH:
- 7
- Temp.:
- 50 °C
- Duration:
- 5 d
- % Recovery:
- 109
- pH:
- 9
- Temp.:
- 50 °C
- Duration:
- 5 d
- Key result
- pH:
- 4
- Temp.:
- 25 °C
- DT50:
- 35 h
- Key result
- pH:
- 7
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Key result
- pH:
- 9
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Details on results:
- Please see Table 1 and 2 below for details on Test 1 and Test 3.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item is hydrolytically stable (t1/2 > 1 year) at pH 7 and 9. At pH 4, the half-life time (t1/2) is 35 h at 25 °C.
- Executive summary:
The determination of the hydrolysis as a function of pH was based on the EEC-Directive 92/69 EEC, Part C, Methods for the determination of Ecotoxicity, C.7: “Abiotic degradation: Hydrolysis as a function of pH”. The test item was determined to be hydrolytically stable (half-life time at 25 °C > 1 year) at pH 7 and pH 9 under the conditions of the test. At pH 4, a decrease in concentration < 50 % after 2.4 hours but > 10% after 5 days was observed. In order to determine if hydrolysis of the test item is a pseudo-first order reaction, a subsequent test was performed. The plot of the logarithms of the relative concentrations between 20 % and 70 % hydrolysis against time was a straight line. Therefore, the reaction at pH4 is considered to be (pseudo)-first order. Hence the half-life time at 25 °C was determined in a subsequent test. At pH 4, the half-life times were 18 h (30.5°C) and 5.9 h (40.7°C). From these values the half-life time at pH 4 and 25 °C was calculated to be 35 h.
Reference
Table 1: Results of Test 1 at 40.7 °C ± 0.1°C
code pH |
Measured pH value |
Time (hours) |
Concentration test substance [g/L]* |
Relative concentration [%] |
logarithm rel. conc. |
4 |
4.0 |
0 |
0.512 |
100 |
2.00 |
4.0 |
1 |
0.467 |
91.3 |
1.96 |
|
4.0 |
2 |
0.409 |
79.9 |
1.901 |
|
4.0 |
3 |
0.368 |
71.9 |
1.861 |
|
4.0 |
3.5 |
0.352 |
68.7 |
1.841 |
|
4.0 |
4 |
0.323 |
63.1 |
1.801 |
|
4.0 |
4.5 |
0.318 |
62.1 |
1.791 |
|
4.0 |
5 |
0.283 |
55.3 |
1.741 |
|
4.0 |
5.5 |
0.27 |
52.8 |
1.721 |
|
4.0 |
6 |
0.254 |
49.6 |
1.701 |
*Mean value of duplicate analysis. The maximum deviation between the responses was < 10%.
1Value between 20% and 70% hydrolysis
Table 2: Results of Test 3 at 30.5 ± 0.1°C
Code pH |
Measured pH value |
Time [hours] |
Concentration test substance*[g/L] |
Relative concentration [%] |
logarithm rel. conc. |
4 |
4.0 |
0 |
0.411 |
100 |
2.00 |
4.0 |
16 |
0.232 |
56.6 |
1.75 |
|
4.0 |
19 |
0.198 |
48.1 |
1.68 |
*Mean value of duplicate analysis. The maximum deviation between the responses was < 10%.
Description of key information
The test item is hydrolytically stable (t1/2 > 1 year) at pH 7 and 9. At pH 4, the half-life time (t1/2) is 35 h at 25 °C (reference 5.1.2 -1).
Key value for chemical safety assessment
Additional information
The determination of the hydrolysis as a function of pH was based on the EEC-Directive 92/69 EEC, Part C, Methods for the determination of Ecotoxicity, C.7: “Abiotic degradation: Hydrolysis as a function of pH”. The test item was determined to be hydrolytically stable (half-life time at 25 °C > 1 year) at pH 7 and pH 9 under the conditions of the test. At pH 4, a decrease in concentration < 50 % after 2.4 hours but > 10% after 5 days was observed. In order to determine if hydrolysis of the test item is a pseudo-first order reaction, a subsequent test was performed. The plot of the logarithms of the relative concentrations between 20 % and 70 % hydrolysis against time was a straight line. Therefore, the reaction at pH4 is considered to be (pseudo)-first order. Hence the half-life time at 25 °C was determined in a subsequent test. At pH 4, the half-life times were 18 h (30.5°C) and 5.9 h (40.7°C). From these values the half-life time at pH 4 and 25 °C was calculated to be 35 h (reference 5.1.2 -1).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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