Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 482-280-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 March 2018 - 04 April 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- Domestic sewage was collected from the final effluent stage of the Severn Trent Water Plc sewage treatment plant at Loughborough.
The sample of effluent was filtered through coarse filter paper and maintained on aeration in a temperature controlled room at 21± 1 °C prior to use.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- DOC
- Remarks:
- Dissolved Organic Carbon (DOC) analysis was performed to confirm that the aniline and test item stock solutions were prepared correctly.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- The following test preparations were prepared and inoculated;
(i) 3 replicate bottles containing innoculated mineral medium to act as the inoculum control.
(ii) 2 replicate bottles containing inoculated mineral medium and the reference item, aniline at a concentration of 100 mg/L
(iii) 3 replicate bottles in inoculated mineral medium and the test item at a concentration of 100 mg/L
(iv) 2 replicate bottles containing inoculated mineral medium plus the reference item, aniline at a concentration of 100 mg/L and the test item at a concentration of 100 mg/L to act as toxicity control vessels.
All vessels were innoculated with the prepared inoculum at a rate of 1% v/v.
On Day 0, the test and reference added and the pH measured. All volumes were adjusted to 500 mL wih medium. All vessels were then placed in a CES Multi-Channel Aerobic Respirometer. The item was suspected to be volatile so the vessels containing test item were place onto the respirometer within 5 minutes of the test item being added so to minimise any losses of test item due to volatility.
The system consisted of a sample flask sealed by a sensor head/CO2 trap immersed in a temperature controlled water bath. The samples were stirred for the duration of the test with a magneticaly coupled stirrer.
The test was conducted in diffuse light at temperatures of between 21 and 23 °C.
On Day 28, the pH of the vessels was measured again.
The daily Biological Oxygen Demand (BOD) values for the inoculum control, test item, procedure control and the toxicity control were measured daily. - Reference substance:
- aniline
- Preliminary study:
- Not applicable
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 4
- Sampling time:
- 28 d
- Details on results:
- The mean BOD of the inoculated mineral medium (control) was 28.24 mg O2/L after 28 days and therefore satisfied the validation criteria.
The pH of the inoculated test vessels on Day 28 were 7.6 and hence satisfied the validation criterion.
The difference between the extremes of replicate BOD values at the end of the test was less than 20% and therefore satisfied the validation criterion.
The test item attained 4% biodegradation after 28 days calculated from the oxygen consumption values and therefore cannot be considered to be readily biodegradable according to OECD Guideline No 301F.
The toxicity control attained 54% biodegradation after 14 days and 55% biodegradation after 28 days thereby confirming that the test item was not toxic to the sewage treatment organisms used in the test. - Results with reference substance:
- Aniline (positive control) attained 76% biodegradation after 14 days and 79% biodegradation after 28 days thereby confirming the suitability of the inoculum and test conditions.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item attained 4% biodegradation after 28 days and therefore cannot be considered as readily biodegradable.
- Executive summary:
The study was performed to assess the ready biodegradability of the test item in an aerobic aqueous media according to OECD method 301F.
The test item at a concentration of 100 mg/L was exposed to sewage treatment microorganisms with mineral medium in sealed culture vessles in diffuse light for 28 days. The biodegradation rate was assessed by the measurement of the daily oxygen consumption values. The test item attained 4% biodegradation after 28 days and therefore cannot be considered as readily biodegradable.
Reference
Description of key information
The test item attained 4% biodegradation after 28 days and therefore cannot be considered as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.