Amines, C12-14-tert-alkyl, compds. with 2(3H)-benzothiazolethione

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02 June 1971 to 09 September 1971

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: U.S. Federal Hazardous Substance Act

Deviations:

no

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

1

Reference substance name:

Amines, C12-14-tert-alkyl, compds. with 2(3H)-benzothiazolethione

EC Number:

272-782-3

EC Name:

Amines, C12-14-tert-alkyl, compds. with 2(3H)-benzothiazolethione

Cas Number:

68911-68-2

Molecular formula:

N/A

IUPAC Name:

C12-14-tert-alkylamines, compds. with 2(3H)-benzothiazolethione

Test material form:

liquid

Specific details on test material used for the study:

- Description: Viscous, amber liquid with an unpleasant odor

Test animals

Species:

rat

Strain:

other: Albino

Sex:

male

Details on test animals or test system and environmental conditions:

- Diet: Commercial pellets
- Water: Available ad libitum
- Housing: Wire mesh cages suspended above droppings

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

Sample administered undiluted

Doses:

Doses of 1.00, 2.15, 4.64, 10.0, and 21.5 mL/kg body weight

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

All animals were observed closely for gross signs of systemic toxicity and mortality at frequent intervals during the day of dosage, and at least once daily thereafter until necropsy.

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

1.47 mL/kg bw

Based on:

test mat.

Mortality:

At the 1.00 mL/kg dose, none of the five rats died during the 14 days of observation. At the 2.15 mL/kg dose, two rats died on the second day and all five died by Day 8. At the 4.64 mL/kg dose, four rats died on Day 2 and fifth rat died on Day 5. At the 10.0 mL/kg and 21.5 mL/kg doses, all five rats died on Day 2 of observation.

Clinical signs:

other: At the 1.00 mL/kg dose, signs included excessive salivation stains in all rats and bloody-appearing stains around the nose or eyes of an occasional rat. Signs persisted through the first post-dosage day. On the second day, all rats exhibited depression,

Gross pathology:

Gross necropsy findings in the rats which died included external evidence of diarrhea, urine stains, excessive salivation stains and bloody-appearing stains around nose, mouth or eyes. Internal changes included congested lungs, kidneys and adrenals; cardiac region of stomach and/or intestines diffusely blanched, irritated or hemorrhagic; areas of blanched tissue "thickened"; peritoneal wall irritated and/or "wrinkled"; body fat stores depleted; and autolytic alterations.

Necropsies performed on the surviving group of rats at termination revealed no gross pathological alterations.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The acute oral LD50 for male albino rats was found to be 1.47 mL/kg of body weight. Confidence limits could not be calculated due to the "all or none" response. The test material is classified as harmful by ingestion.

Executive summary:

The acute oral toxicity of the test material was evaluated in accordance with the techniques specified in the Regulations for the Enforcement of the Federal Hazardous Substances Act (Revised, Federal Register, September 17, 1964). The acute oral LD50 for male albino rats was found to be 1.47 mL/kg of body weight.  Confidence limits could not be calculated due to the "all or none" response.  The test material is classified as harmful by ingestion.