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EC number: 246-237-5 | CAS number: 24413-21-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- GLP compliance:
- yes
Test material
- Reference substance name:
- Guanidinium phosphate (1:1)
- EC Number:
- 226-551-9
- EC Name:
- Guanidinium phosphate (1:1)
- Cas Number:
- 5423-22-3
- Molecular formula:
- CH5N3.H3O4P
- IUPAC Name:
- Guanidium phosphate (1:1)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Blank control and 100 mg/L
- Sampling
Frequency: at t=0 h and t=48 h
Volume: 1 mL
- Sample storage conditions before analysis: Samples were transported on dry ice to TNO Triskelion (P.O. Box 844, 3700 AV, Zeist, The Netherlands) and were stored in a freezer until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Preparation of test solutions started with a nominal concentration of 100 mg/L applying a short period of vigorous shaking to accelerate the dissolving of the test substance in the test medium.
- Controls: Blank control containing test medium without test substance or other additives
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The final test solution was clear and colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: In-house laboratory culture with a known history
- Age at study initiation: <24 h
- Feeding during test: None
BREEDING:
- Method: At least third generation obtained by acyclical parthenogenesis under specified breeding conditions. Each batch started with newborn daphnids (i.e. less than three days old) and the maximum age of the cultures was 4 weeks
- Medium: M7 prepared according to Elendt, B.-P., 1990: Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus. Protoplasma 154, 25-33.
- Conditions (same as test or not): yes
- Type of food: suspension of fresh water algae
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/L expressed as CaCO3
- Test temperature:
- Start: 20.3 to 20.5 °C
During test period: 19.4 - 19.9 °C - pH:
- Start: 6.8 - 7.8
End: 7.4 - 8.2 - Dissolved oxygen:
- Start: 9.2 - 9.3 mg/L
End: 8.9 mg/L - Nominal and measured concentrations:
- - Nominal: blank control, 0.1, 1, 10 and 100 mg/L
- Measured
t=0h: <0.08 mg/L* (blank control) and 97 mg/L (100 mg/L).
t=48h: <0.08 mg/L* (blank control) and 107 mg/L (100 mg/L)
*) LOD was 0.08 mg/L (lowest calibration point). - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL, all glass, containing 80 mL of test solution
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 for the highest test concentration (100 mg/L) and 2 for each lower concentration (0.1 - 10 mg/L)
- No. of vessels per control (replicates): 4
- Loading: 5 daphnids per 80 mL of test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water purified by Reverse Osmosis
- Culture medium different from test medium: Yes
- Intervals of water quality measurement:
pH was measured at the beginning of the test for the control and all test concentrations. At the end of the test for the control and the highest test concentration.
Dissolved oxygen was measured at the beginning and at the end of the test for both the control and the highest test concentration.
Temperature was measured continuously in a temperature control vessel.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light : 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobility (including mortality) at 24 and 48 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: x10 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 33 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: guanidine content (32.7% w/w)
- Basis for effect:
- mobility
- Details on results:
- - Measured concentrations: Analysis of the samples taken from the 100 mg/L concentration showed that the actual concentration was in agreement with nominal and remained stable during the test period (97-107%). Given this result the EC50-value was based on the nominal test concentration.
- Biological results: A single daphnid was found immobilized at the highest test concentration (100 mg/L). For the lower test concentrations and the blank control no immobilized daphnids were observed.
- Acceptability of the test:
1. In the control, no daphnids became immobilised.
2. The oxygen concentration at the end of the test was ≥3 mg/L in control and test vessels. - Results with reference substance (positive control):
- - Potassium dichromate (K2Cr2O7): The 48h-EC50 was 0.53 mg/L (95% confidence interval between 0.49 and 0.62 mg/L)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48h-EC50 for Daphnia magna exposed to Guanidine phosphate (1:1) was >100 mg/L
- Executive summary:
In a 48-h acute toxicity study conducted according to OECD guideline 202, water fleas (Daphnia magna) were exposed to Guanidine phosphate (1:1) under static conditions at the following nominal concentrations: 0 (blank control), 0.1, 1, 10 and 100 mg/L. The test substance induced no immobilization in daphnids at a nominal exposure concentration of 100 mg/L. The 48h-EC50 was >100 mg/L (corresponding to >33 mg guanidine/L) based on analytically confirmed nominal concentrations. The study is considered to be reliable without restrictions.
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