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EC number: 261-675-7 | CAS number: 59231-37-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 May 2017 to 9 October 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- OECD Guideline 301B, Ready Biodegradability
(Adopted 1981, Revised 1992) - Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Isotridecyl 3,5,5-trimethylhexanoate
- EC Number:
- 261-675-7
- EC Name:
- Isotridecyl 3,5,5-trimethylhexanoate
- Cas Number:
- 59231-37-7
- Molecular formula:
- C22H44O2
- IUPAC Name:
- isotridecyl 3,5,5-trimethylhexanoate
- Test material form:
- liquid
1
- Specific details on test material used for the study:
- Test Substance
The following details for the test substance, Isotridecyl 3,5,5-trimethylhexanoate, were supplied by the Sponsor:
Test substance name: Isotridecyl 3,5,5-trimethylhexanoate
Synonym: Wickenol153 and Isotridecyl Isononanoate
Molecular formula: C22H44O2
Molecular weight: 340.59 g/mol
Batch number: P7639
Purity: 100.2%
Date received: 1 March 2017
Retest date: 1 December 2018
Storage conditions: Room temperature (15-30°C) protected from light
All test substance details were supplied by the Sponsor. An analysis report is presented in report Appendix 1.
The carbon content, 78%, was calculated from the molecular weight provided by the Sponsor. This carbon content was used in setting the test concentration of Isotridecyl 3,5,5-trimethylhexanoate. No allowance was made for purity.
A solubility trial was performed by weighing a sub-sample (290 mg calculated quantity to ensure 225 mg carbon) of Isotridecyl 3,5,5-trimethylhexanoate into a suitable container and making up to 100 mL with reverse osmosis (RO) water. The test substance did not appear to be in solution and was sonicated for 5 minutes and stirred for 10 minutes. As the test substance was not in solution, it was deemed insufficiently soluble to be added as an aqueous stock solution. Isotridecyl 3,5,5trimethylhexanoate was therefore added directly to the test vessels.
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- A sample of activated sludge was collected from one of the return lines at Burley Menston sewage treatment works (West Yorkshire, UK), which has a predominantly domestic waste-water catchment. The sample was transported in a closed container, but with an adequate headspace, to prevent the sample becoming anaerobic. On arrival, the sample was aerated by means of a compressed air supply.
The suspended solids concentration of the activated sludge was determined by filtering a subsample (25 mL) through a pre-dried and pre-weighed glass microfibre filter (Whatman GF/C). The filter and retained solids were then dried in an oven (nominally 105°C) and re-weighed. The weight of the sludge solids was determined from the difference in the weights before and after drying. The concentration of suspended solids was calculated to be 6.2 g/L.
The activated sludge used in this study was not deliberately acclimatised or adapted to Isotridecyl 3,5,5-trimethylhexanoate before exposure under test conditions. - Duration of test (contact time):
- ca. 29 d
Initial test substance concentration
- Initial conc.:
- ca. 15 mg/L
- Based on:
- IC (inorganic carbon)
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- - Preparation of Test Vessels:
The study consisted of four treatment groups as described in the following table:
Treatment Group Vessel Contents
Blank control inoculated mineral salts medium
Test substance inoculated mineral salts medium and test substance
Reference substance inoculated mineral salts medium and sodium benzoate
Toxicity control inoculated mineral salts medium, test substance and sodium benzoate
The purpose of the toxicity control was to assess the biodegradation of the reference substance in the presence of the test substance.
Duplicate vessels were prepared for the test substance, reference substance and blank control groups. A single vessel was prepared for the toxicity control.
Preparation of Test Medium.
The test was conducted in an aqueous, synthetic, mineral salts medium. A test medium concentrate was prepared in RO water containing 30 mL/L solution (a) and 3 mL/L of each of solutions (b), (c) and (d). Solutions (a) to (d) were prepared as follows:
(a) potassium dihydrogen phosphate (8.50 g, VWR); dipotassium hydrogen phosphate(21.75 g, VWR); disodium hydrogen phosphate dihydrate (33.40 g, Fisher); ammonium chloride (0.50 g, Fisher), all dissolved in and made up to 1 L with RO water.
(b) calcium chloride dihydrate (36.40 g, VWR), dissolved in and made up to 1 L withRO water.
(c) magnesium sulphate heptahydrate (22.50 g, Sigma-Aldrich), dissolved in andmade up to 1 L with RO water.
(d) ferric chloride hexahydrate (0.25 g, Sigma-Aldrich) and concentrated hydrochloricacid (1 drop, VWR), dissolved in and made up to 1 L with RO water.
On the basis of the suspended solids determined to be 6.2 g/L, the medium was inoculated with activated sludge (116 mL in a total volume of 8 L) to give a suspended solids concentration of 90 mg/L. This provided a nominal final solids concentration of 30 mg/L in each test vessel (500 mL added to a total volume of 1.5 L).
The inorganic carbon (IC) concentration of the inoculated mineral salts medium was determined using an InnovOx carbon analyser. In this analysis, IC in the samples was released as CO2 by acidification with hydrochloric acid. The CO2 was then passed to a non-dispersive infra-red (NDIR) detector. The concentration of carbon dioxide was determined in the NDIR detector, by measuring the amount of infra-red energy absorbed by the sample. A calibration check was performed on each occasion by injecting a series of sodium hydrogen carbonate standards. The existing calibration curve was used to quantify the IC present in the samples. Each sample was analysed in triplicate/quadriplicate.
Treatment of Test Vessels
Isotridecyl 3,5,5-trimethylhexanoate was accurately weighed (29.03 to 29.14 mg) for direct addition to test substance and toxicity control vessels, to give a nominal test substance concentration corresponding to 15 mg carbon/L.
A reference substance stock solution (2.25 g carbon/L) was prepared by dissolving sodium benzoate (1.93 g) in RO water (500 mL). Reference and toxicity control vessels were treated with the stock solution (10 mL), to give a nominal sodium benzoate concentration corresponding to 15 mg carbon/L.
- Test Initiation:
Following all test and reference substance treatments and addition of the inoculated medium concentrate, the volume in each vessel (including the blank control vessels) was made up to 1.5 L by addition of RO water. Each vessel was sealed, connected to a series of three traps containing aqueous barium hydroxide (nominally 0.0125 M), and the carbon dioxide-free air supply initiated.
Incubation Conditions
The test vessels were incubated in the dark under the conditions below.
- Temperature:
The incubation and test measurements were conducted at a target temperature range of 22 ± 2°C. During the course of the study, the temperature decreased to 17.6°C. Thereafter temperature settled back to target range. This did not appear to affect the study integrity.
- Measurement of pH:
Measurements of pH were made in the blank control and reference substance vessels at the start of incubation and in all vessels at the end of the test prior to the addition of the hydrochloric acid. Measured pH values ranged from pH 7.54 to pH 7.66 on Day 0 and pH 7.44 to pH 7.61 on Day 28 (Table 1).
- Air Flow:
The air used in this study was delivered from a cylinder of CO2-free air (Air Products) and was regulated in two stages. Initial control was provided by a gas regulator and the air flow to each vessel controlled by individual needle valves. Measurements of the flow rate exiting each test vessel were made at intervals not exceeding seven days, with a bubble flow meter and stopwatch. Adjustments were made as necessary to maintain a flow rate of ca 50 mL per minute.
- Calculations:
Theoretical CO2 Yields
The theoretical yield of carbon dioxide (TCO2 in mg) from cultures containing the test and/or reference substances was calculated as shown below.
TCO2 = D(abs) x Pc x 3.667 where:
D(abs) = the absolute dose i.e. the amount (mg) of test or reference substance added to the culture
Pc = the percentage carbon content of the test or reference substance 3.667 = the weight (mg) of CO2 produced from 1 mg of carbon.
The theoretical CO2 yield for the quantity of Isotridecyl 3,5,5-trimethylhexanoate applied to the test vessels and toxicity control vessel was 82.5 mg CO2. Theoretical yields for the quantities of applied sodium benzoate in the toxicity control vessel and reference vessels were also 82.5 mg CO2. The combined theoretical yield from the toxicity control vessel was 165 mg CO2. The purpose of this control was not to assess the extent of degradation of the entire mixture, but instead to assess the impact of the presence of the test substance on the degradation of the reference material. The theoretical yield in this case was, therefore, limited to the 82.5 mg CO2 expected from the sodium benzoate alone and the actual yield corrected for that from the test substance.
Measured CO2 Yields as a Percentage of Theoretical
Biodegradation (Dt) of the reference substance and of Isotridecyl 3,5,5trimethylhexanoate expressed in terms of percentage theoretical CO2 yield was calculated by applying the formula:
Dt = (cumulative mf CO2 produced at time (t))/82.5 x 100
The cumulative CO2 values for the test substance, reference substance and toxicity control were corrected for the mean CO2 generated by the blank controls, to determine the percent biodegradation.
- Validity Criteria:
The following criteria were required for the study to be considered valid:
• the IC content of the mineral medium was less than 5% of the total carbon content at the beginning of the test
• degradation of the reference material reached 60% by Day 14
• duplicate percentage biodegradation values for vessels containing the test substance differ from one another by less than 20%
• total CO2 production from each blank control vessel did not exceed 70 mg/L
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- The inorganic carbon content of the test medium was determined to be 1.93 mg carbon/L, corresponding to 4.29%.
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- <= 47
- Sampling time:
- 29 d
- Details on results:
- - Measured CO2 Yields as a Percentage of Theoretical:
Carbon dioxide evolution and percentage biodegradation data are presented in Table 2, Table 3 and Figure 1. The mean total carbon dioxide production in the blank control vessels was 43 mg/L (Test end), satisfying the validity criterion of less than 70 mg/L.
- Isotridecyl 3,5,5-trimethylhexanoate:
To be considered readily biodegradable, a test substance must achieve 60% biodegradation by the end of the test. Additionally, the test substance must biodegrade by at least 60% within 10 days of having degraded by 10%.
Isotridecyl 3,5,5-trimethylhexanoate showed limited biodegradation during the study with a maximum mean biodegradation of 47%. Isotridecyl 3,5,5-trimethylhexanoate cannot, therefore, be considered readily biodegradable.
Percent biodegradation values at each sampling interval, for the two replicates containing Isotridecyl 3,5,5-trimethylhexanoate did not vary by more than 14%, therefore satisfying the validity criterion of less than 20% difference.
- Sodium Benzoate:
Rapid carbon dioxide generation commenced immediately and declined to a more gradual rate over the period of the incubation as shown in Figure 1. The mean percentage biodegradation had exceeded 60% by Day 7 (66%), 85% by the end of the incubation phase on Day 28 and 86% by the end of the study on Day 29. The validity criterion of 60% biodegradation at 14 days was therefore met.
- Toxicity Control:
Assessment of biodegradation in the toxicity control was confined to the sodium benzoate fraction. The rate of biodegradation of the reference substance in the presence of Isotridecyl 3,5,5-trimethylhexanoate (63% at Day 7, 75% at the end of the incubation phase on Day 28 and 76% by the end of the test on Day 29) was higher than that of the reference substance alone. This suggests that Isotridecyl 3,5,5 trimethylhexanoate had no inhibitory effect on the sludge microorganisms under the test, the test was considered to be valid.
BOD5 / COD results
- Results with reference substance:
- - Sodium Benzoate:
Rapid carbon dioxide generation commenced immediately and declined to a more gradual rate over the period of the incubation as shown in Figure 1. The mean percentage biodegradation had exceeded 60% by Day 7 (66%), 85% by the end of the incubation phase on Day 28 and 86% by the end of the study on Day 29. The validity criterion of 60% biodegradation at 14 days was therefore met.
- Toxicity Control:
Assessment of biodegradation in the toxicity control was confined to the sodium benzoate fraction. The rate of biodegradation of the reference substance in the presence of Isotridecyl 3,5,5-trimethylhexanoate (63% at Day 7, 75% at the end of the incubation phase on Day 28 and 76% by the end of the test on Day 29) was higher than that of the reference substance alone. This suggests that Isotridecyl 3,5,5trimethylhexanoate had no inhibitory effect on the sludge microorganisms under the test, the test was considered to be valid.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- All validity criteria were satisfied (inorganic carbon content, degradation of reference material, CO2 production from blank controls) and the results of this study are therefore considered to be valid.
No inhibitory effect was observed on the biodegradation of the reference substance in the presence of Isotridecyl 3,5,5-trimethylhexanoate.
Mean carbon dioxide evolution from Isotridecyl 3,5,5-trimethylhexanoate was < 60% of the theoretical carbon dioxide yield throughout the test. The level of biodegradation did not meet the requirements for ready biodegradability and Isotridecyl 3,5,5 trimethylhexanoate cannot, therefore, be classified as readily biodegradable. - Executive summary:
The ready biodegradability of Isotridecyl 3,5,5-trimethylhexanoate was assessed by measurement of carbon dioxide (CO2) evolution under standard conditions. The procedure followed was that of OECD Guideline 301B, Ready Biodegradability (Adopted 1981, Revised 1992).
The test substance, Isotridecyl 3,5,5-trimethylhexanoate, was added to the test system as a direct addition. Buffered mineral salts medium was added to give a test substance concentration equivalent to 15 mg organic carbon/L. The medium was inoculated with microorganisms derived from a sample of activated sludge not previously intentionally exposed to the test substance. Test vessels were incubated in darkness at 22 ± 2°C for 28 days and their contents continuously sparged with a supply of CO2-free air. The exhaust air from each vessel was passed through a series of traps containing a barium hydroxide solution to trap evolved CO2.
At regular intervals during the incubation, traps were detached and their contents titrated with hydrochloric acid to determine the quantity of CO2evolved from the respective test vessels. At the end of incubation, 28 days, the test vessel contents were acidified to release any residual CO2that may have remained in solution. Titration of the traps was performed following overnight aeration.
The procedure and the activity of the inoculum were checked by measuring the CO2evolved from vessels containing a reference substance, sodium benzoate. An additional vessel containing a combination of the test and reference substances served as a toxicity control to assess whether the test substance was inhibitory to biodegradation at the test concentration. Two blank control vessels were also prepared containing inoculated medium only. The results of these vessels were used to check the validity of the test and to correct the evolved CO2values.
Isotridecyl 3,5,5-trimethylhexanoate showed < 60% biodegradation during the incubation. As a result, Isotridecyl 3,5,5-trimethylhexanoate cannot be considered readily biodegradable.
The mean total CO2production in the blank control vessels was 43 mg/L at the end of the test, satisfying the validity criterion of < 70 mg/L.
Mean biodegradation of the reference substance had exceeded 60% by Day 7 and had reached 85% by the end of the incubation phase on Day 28 and 86% at the end of the test on Day 29. The corrected rate of biodegradation of the reference substance in the presence of Isotridecyl 3,5,5-trimethylhexanoate (63 % at Day 7, 75% by the end of the incubation phase on Day 28 and 76% at the end of the test on Day 29 test) was similar to that of the reference substance alone, suggesting that Isotridecyl 3,5,5trimethylhexanoate did not have an inhibitory effect on the sludge microorganisms under the test conditions and the test was considered to be valid.
The inorganic carbon content of the test medium was determined to be
1.93 mg carbon/L, corresponding to 4.29% satisfying the validity criterion of < 5%.
All validity criteria were satisfied, the blank controls and reference vessels performed as expected, and the results of this study are therefore considered to be valid.
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