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EC number: 445-090-6 | CAS number: 5614-37-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish, juvenile growth test
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2013-01-28 to 2013-06-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 215 (Fish, Juvenile Growth Test)
- Version / remarks:
- 2000
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch No.: 2820246
Purity: ≥ 99.9% - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 12.5, 25, 50, 100, 200 mg/L
- Sampling method: For analysis of the test concentration, 30 mL of test solution was taken from each test vessel at the first week before and after each renewal, twice a week before and after renewal in the following weeks and at the end of the test.
- Sample storage conditions before analysis: analyzed directly without storage - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 64.0 g, 64.0 g, 64.1 g and 64.0 g test substance were dissolved in test water to prepare the stock solution of 8 g/L. Then based on the measured concentrations, the test solutions were prepared by dilution of the stock solution with test water.
- Controls: blank control of test water - Test organisms (species):
- other: Gobiocypris rarus
- Details on test organisms:
- TEST ORGANISM
- Common name: Chinese Rare Minnow
- Source: Shanghai Academy of Public Measurement
- Weight at study initiation (mean and range, SD): 0.091 g ± 0.011 g (Mean±SD)
- Method of breeding: fish were fed with Brine Shrimp Aquarium Flake Food twice a day and food was withhold from the fish for 24 hours before weighing at the start of the test.
FEEDING DURING TEST
- Feeding:fish were fed twice a day and two feeds were separated by 6 hours. The quantity of food given to fish was with a rate of about 4% of body weight per day. Food was withheld from the fiish for 24 hours prior to weighing. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
- Hardness:
- 170 mg CaCO3/L in the control
186 mg CaCO3/L in the highest concentration - Test temperature:
- 22.8-23.7 °C
- pH:
- 7.02-7.85
- Dissolved oxygen:
- 60.2% - 93.8% ASV
- Nominal and measured concentrations:
- Nominal: 12.5, 25, 50, 100, 200 mg/L
Measured: 13.5, 25.4, 50.7, 95.6 and 196 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10L glass bottle
- Type: closed
- Aeration: no
- Renewal rate of test solution: 24 h
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.18-0.28 g fish/litre darning the test
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The tap water dechlorinated by activated carbon and sterilized by UV lamp was used. The water was aerated continuously over 24h.
- Total hardness: 155 mg CaCO3 per liter;
- Oxygen concentration: >90% of ASV.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: Light: Dark= 12 hours:12 hours
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The fish were inspected and recorded once a day for mortality and other observed effects. Dead fish were removed when observed and the feeding rate was adjusted.
Total hardness was measured in the controls and one vessel at the highest concentration once during the test.
Measurements of pH, dissolved oxygen and temperature were carried out before and after each renewal. - Reference substance (positive control):
- no
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 50.7 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 95.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Test concentration: In the test, the measured values were within ± 20% of the mean concentrations in each replicate.
- Body weight: In the test, the body weights of the test fish increased in all groups.
- - Growth rate: The specific growth rate of the 95.0 mg/L and 196 mg/L treatment groups were significantly lower than that of the control (p<0.05), the specific growth rate of other treatment groups were not significantly different from that of the control.
- Mortality and abnormality: During the test, there were no mortality and abnormality on fish in treatment groups. - Reported statistics and error estimates:
- To determine the lowest observed effect concentration (LOEC) and the no observed effect concentration (NOEC), the variances among the means of the specific growth rate in all groups was analyzed by one-way ANOVA and the mean of treatment groups was compared with the control group using Bonferroni t test. The data treatment was carried out with software TOXCALC (v5.0.26). The results were expressed as geometic mean average of measured concentration of solutions.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The no observed effect concentration (NOEC) was 50.7 mg/L and the lowest observed effect concentration (LOEC) was 95.6 mg/L (expressed as the geometric mean measured concentration of test solutions).
- Executive summary:
The effect on growth of juvenile fish of the test substance to Chinese Rare Minnow (Gobiocypris rarus} was determined by a closed semi-static test according to OECD 215.
In the test, five treatment groups and a control group were performed. The nominal concentrations of the treatment groups were 12.5 mg/L, 25 mg/L, 50 mg/L,100 mg/L and 200 mg/L(which the mean measured concentrations of the treatment groups were 13.5 mg/L, 25.4 mg/L, 50.7 mg/L, 95.6 mg/L, 196 mg/L). All treatment groups and control group had no replicates with 20 fish and the test duration was 28 days.
In the test, the measured values were within ± 20% of the mean concentrations in each replicate.
During the test, there were no mortality and abnormality on fish in treatment groups.
The no observed effect concentration (NOEC) was 50.7 mg/L and the lowest observed effect concentration (LOEC) was 95.6 mg/L (expressed as the geometric mean measured concentration of test solutions).
Reference
Description of key information
The no observed effect concentration (NOEC) was 50.7 mg/L and the lowest observed effect concentration (LOEC) was 95.6 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 50.7 mg/L
Additional information
The effect on growth of juvenile fish of the test substance to Chinese Rare Minnow (Gobiocypris rarus) was determined by a closed semi-static test according to OECD 215.
In the test, five treatment groups and a control group were performed. The nominal concentrations of the treatment groups were 12.5 mg/L, 25 mg/L, 50 mg/L,100 mg/L and 200 mg/L(which the mean measured concentrations of the treatment groups were 13.5 mg/L, 25.4 mg/L, 50.7 mg/L, 95.6 mg/L, 196 mg/L). All treatment groups and control group had no replicates with 20 fish and the test duration was 28 days.
In the test, the measured values were within ± 20% of the mean concentrations in each replicate.
During the test, there were no mortality and abnormality on fish in treatment groups.
The no observed effect concentration (NOEC) was 50.7 mg/L and the lowest observed effect concentration (LOEC) was 95.6 mg/L (expressed as the geometric mean measured concentration of test solutions).
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