Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 236-759-1 | CAS number: 13476-99-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction
Administrative data
- Endpoint:
- fertility, other
- Remarks:
- Fertility Assessment Test (Sperm assessment)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- in vivo mammalian cell study: DNA damage and/or repair
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- For the evaluation of DNA damage in the germ cells in COMET assay, CD-1 male mice (30-45 days old, 30-35 g, from our own stock) were housed in groups of two (experimental and control) in hanging plastic cages under controlled lighting conditions (lights on from 05:00 to 19:00 h). They were fed rat chow and water ad libitum. Working solutions of V2O5 were prepared in saline and injected i.p. in appropriate volumes containing either 5.75, 11.50, or 23 mg of V2O5/ kg body weight (1/4 of LD50. 1/2 of LD50, or LD50, determined in our laboratory for acute treatments).
Twenty-four hours after treatment, the animals were killed and the testes dissected and stored in 1 ml of RPMI medium (Sigma Chemical Co.) and minced in 2 ml cold saline. The cells were obtained and placed in 75 µL of low melting point agarose. SCG assay was performed as described by Tice et al. (1992). Briefly, after lysis at 4°C for 1 hour, slides were placed on horizontal electrophoresis unit. The DNA was allowed to unwind for 20 min in electrophoresis running buffer solution (300 mM NaOH and 1 mM Na.EDTA, pH 13). Electrophoresis was conducted for 20 min at 25 V and 300 mA. All technical steps were conducted using very dim indirect light. After electrophoresis, the slides were gently removed and alkaline pH neutralized with 0.4 M Tris, pH 7.5. Ethidium bromide (75 µL of a 20 µg/mL solution) was added to each slide and a coverglass was placed on the gel. - GLP compliance:
- no
- Type of assay:
- mammalian comet assay
- Specific details on test material used for the study:
- Working solutions of V2O5 were prepared in saline
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: own stock (Laboratorio de Citogenética. Mutagénesis y Toxicología Reproductiva, UIBR Campo-ll, FES-Zaragoza (M.A.-L. L.A.-B), Laboratorio de Inmunología, Facultad de Medicina Veterinaria y Zootecnia (F.B -A.), and Laboratorio de Genética Toxicológica Molecular, Departamento de GTA Institutode Investigaciones Biomédicas (M V, E.R.), UNAM, México, D.F, México)
- Age at study initiation: 30-45 d
- Weight at study initiation: 30-35 g
- Assigned to test groups randomly: not specified
- Fasting period before study: not specified
- Housing: hanging plastic cages (groups of two)
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: not specified
ENVIRONMENTAL CONDITIONS
- Temperature (°C): not specified
- Humidity (%): not specified
- Air changes (per hr): not specified
- Photoperiod (hrs dark / hrs light): 10/14
- Route of administration:
- intraperitoneal
- Vehicle:
- - Vehicle(s)/solvent(s) used: saline
- Concentration of test material in vehicle: 5.75, 11.50, or 23 mg of V2O5/kg body weight - Details on exposure:
- Working solutions of V2O5 were prepared in saline and injected i.p. in appropriate volumes containing either 5.75, 11.50, or 23 mg of V2O5/kg body weight (1/4 of LD50, 1/2 of LD50, or LD50, determined in the laboratory for acute treatments)
- Duration of treatment / exposure:
- one treatment (injection)
- Frequency of treatment:
- one treatment
- Post exposure period:
- 24 h
- Dose / conc.:
- 5.75 other: mg/kg bw
- Dose / conc.:
- 11.5 other: mg/kg bw
- Dose / conc.:
- 23 other: mg/kg bw
- Dose / conc.:
- 0 other: mg/kg bw
- No. of animals per sex per dose:
- 1 (treatments), 3 (control)
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- not specified
- Tissues and cell types examined:
- testes; microscopic analysis revealed the presence of two distinct subpopulations of cells: large cells (mean diameter 67.53 µm) and small cells (mean diameter 45.8 µm)
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: 1/4 of LD50, 1/2 of LD50, or LD50, determined in the laboratory for acute treatments
TREATMENT AND SAMPLING TIMES ( in addition to information in specific fields): 24 hours after treatment
DETAILS OF SLIDE PREPARATION: The testes were dissected and stored in 1 ml of RPMI medium (Sigma Chemical Co.) and minced in 2 ml cold saline. The cells were obtained and placed in 75 µL of low melting point agarose. SCG assay was performed as described by Tice et al. Briefly, after lysis at 4°C for 1 hour, slides were placed on horizontal electrophoresis unit. The DNA was allowed to unwind for 20 min in electrophoresis running buffer solution (300 mM NaOH and 1 mM NaEDTA, pH 13). Electrophoresis was conducted for 20 min at 25 V and 300 mA. All technical steps were conducted using very dim indirect light. After electrophoresis, the slides were gently removed and alkaline pH neutralized with 0.4 M Tris, pH 7.5. Ethidium bromide (75 µL of a 20 µg/ml solution) was added to each slide and a coverglass was placed on the gel.
The staining of the liberated DNA allowed the microscopic discrimination of cell images with and without damage.
METHOD OF ANALYSIS: The microscopic images revealed circular shapes (undamaged DNA) and "COMET" structures (damaged DNA). The extension of each image, signifying the migration distance of DNA, was determined by scaled ocular. The image length of DNA migration (in µm) was determined from 50 cells per slide and 2 slides per concentration.
- Evaluation criteria:
- Other criteria for evaluation were to assign the evaluated cells to classes according to their degree of DNA damage. The classes were low damage(<20%), medium damage (20—10%), high damage (41-95%), and total damage (>95%) according to Anderson et al..
- Statistics:
- nonparametric Wilcoxon rank-sum test was used because this method takes into account all categories of damage.
- Key result
- Sex:
- male
- Genotoxicity:
- positive
- Toxicity:
- not examined
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Additional information on results:
- Figures 1 and 2 and table 1 represent the effect of vanadium pentoxide or saline on the DNA damage index on testicular germ cells, calculated after the measurements by COMET assay. DNA migration increased significantly depending on the dose of vanadium in both large and small cells. The degree of DNA damage in large and small cells was larger in those animals injected with the higher dose of V2O5. The results presented herein show that vanadium treatment induced SSB in DNA of testis cells.
In combination with the reproductive toxicity study (please refer to section 7.8.1), the results presented herein show that vanadium treatment induced SSB in DNA of testis cells, sperm head abnormalities and a low frequency of DLM (dominant lethal mutations). The DLM in males was measured by determining the frequency of live vs. dead and resorbed fetuses occurring after mating chemical-treated males with untreated females. - Conclusions:
- The results presented herein show that vanadium treatment induced SSB in DNA of testis cells. In combination with the reproductive toxicity study (please refer to section 7.8.1), the results presented herein show that vanadium treatment induced SSB in DNA of testis cells, sperm head abnormalities and a low frequency of DLM (dominant lethal mutations). The DLM in males was measured by determining the frequency of live vs. dead and resorbed fetuses occurring after mating chemical-treated males with untreated females. However, it should be noted that gonads contain a mixture of somatic and germ cells. For this reason, positive results in whole gonad (testis) are not necessarily reflective of germ cell damage; nevertheless, they indicate that tested chemical(s) and/or its metabolites have reached the gonad (OECD TG 489).
- Executive summary:
Effects of vanadium pentoxide (V2O5) treatment on reproductive function and testicular DNA in male mice were investigated. These functions were evaluated with fertility rate, implants, resorptions, sperm counts, motility, and morphology. The DNA damage in individual testis cells was analyzed by single-cell gel electrophoresis technique (COMET assay). V2O5 treatment resulted in a decrease in fertility rate, implantations, live fetuses, and fetal weight, and an increase in the number of resorptions/dam. Sperm count, motility, and morphology were impaired with the advancement of treatment. Vanadium treatment induced DNA damage depending on the dose in the testis cells that was expressed and detected as DNA migration in the COMET assay. The distribution of DNA migration among cells, a function of dose, revealed that the majority of cells of treated animals expressed more DNA damage than cells from control animals. It is concluded that vanadium pentoxide was a reprotoxic and genotoxic agent in mice.
Table 1: Effects of Vanadium pentoxide on Mouse Testis Cells in the COMET Assay
|
Grade of damage in large cells (%) |
Grade of damage in small cells (%) |
||||||||
Treatment |
None (<5 %) |
Low (5-20 %) |
Medium (21-40 %) |
High (41-95 %) |
Total (>95 %) |
None (<5 %) |
Low (5-20 %) |
Medium (21-40 %) |
High (41-95 %) |
Total (>95 %) |
Control |
58 |
22 |
20 |
0 |
0 |
68 |
14 |
14 |
4 |
0 |
5.75*** |
16 |
22 |
16 |
40 |
6 |
4 |
4 |
30 |
56 |
6 |
11.50* |
14 |
0 |
20 |
56 |
10 |
52 |
30 |
14 |
4 |
0 |
23.00*** |
1 |
0 |
22 |
76 |
0 |
0 |
8 |
26 |
60 |
6 |
*P<0.05 for large cells vs. control
**P<0.05 for small cells vs. control
Data source
Reference
- Reference Type:
- publication
- Title:
- Reprotoxic and Genotoxic Studies of Vanadium Pentoxide in Male Mice
- Author:
- Altamirano-Lozano M
- Year:
- 1 996
- Bibliographic source:
- Teratogenesis, Carcinogenesis, and Mutagenesis 16:7-17 (1996)
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- - Principle of test:
Effects of vanadium pentoxide (V2O5) treatment on reproductive function and testicular DNA in male mice were investigated.
- Short description of test conditions: 8.5 µg/g bw V2O5 was administered to male rats intraperitoneally for 60 days. On day 61 they were subjected to a fertility assessment test
- Parameters analysed / observed: The fertility of male mice was assessed by the incidence of pregnancy in females. Other reproductive parameters determined were the number of litters, implants, resorptions, live and dead fetuses, and fetal body weight. In addition, sperm motility, sperm counts and sperm morphology was evaluated. - GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Divanadium pentaoxide
- EC Number:
- 215-239-8
- EC Name:
- Divanadium pentaoxide
- Cas Number:
- 1314-62-1
- Molecular formula:
- O5V2
- IUPAC Name:
- dioxovanadiooxy(dioxo)vanadium
- Details on test material:
- Aldrich Chemical Co., Milwaukee, WI
Constituent 1
- Specific details on test material used for the study:
- A working solution of vanadium pentoxide (99.6% pure, Aldrich Chemical Co., Milwaukee, WI, CAS 1314-62-1) was prepared by dissolving the compound in saline.
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: own stock (Laboratorio de Citogenética. Mutagénesis y Toxicología Reproductiva, UIBR Campo-ll, FES-Zaragoza (M.A.-L. L.A.-B), Laboratorio de Inmunología, Facultad de Medicina Veterinaria y Zootecnia (F.B -A.), and Laboratorio de Genética Toxicológica Molecular, Departamento de GTA Institutode Investigaciones Biomédicas (M V, E.R.), UNAM, México, D.F, México)
- Age at study initiation: 45 d
- Weight at study initiation: (P) Males: 26-29 g;
- Housing: hanging plastic cages
- Diet (e.g. ad libitum): ad libitum, Purina chow
- Water (e.g. ad libitum): ad libitum
- Acclimation period: not specified
ENVIRONMENTAL CONDITIONS
- Temperature (°C): not specified
- Humidity (%): not specified
- Air changes (per hr): not specified
- Photoperiod (hrs dark / hrs light): 10/14
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- water
- Remarks:
- saline
- Details on exposure:
- A working solution of vanadium pentoxide was prepared by dissolving the compound in saline and injected intraperitoneally (i.p.) in an appropriate volume containing 8.5 µg/g body weight. Controls were treated with saline.
- Details on mating procedure:
- - M/F ratio per cage: 1/2
- Length of cohabitation: 5 overnight matings
- Proof of pregnancy: vaginal plug and/or sperm in vaginal smear - Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 60 days
- Frequency of treatment:
- every third day
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0 other: µg/g bw/0.3 d
- Dose / conc.:
- 8.5 other: µg/g bw/0.3 d
- No. of animals per sex per dose:
- Group A (vehicle control): 20 males
Group B: 15 animals were injected with V2O5 every 3rd day during 60 days (fertility assessment)
Groups C-H: 30 animals were injected with V2O5 every 3rd day. Groups of 5 animals were sacrificed every 10 days after the beginning of the treatment (sperm assessment) - Control animals:
- yes, concurrent vehicle
- Details on study design:
- Sixty-five males that proved fertile were allotted randomly to the following experimental groups. Group A: controls (20 animals) were injected with vehicle every 3rd day during 60 days. Beginning on day 61, they were subjected to a fertility assessment test and sacrificed 5 days later. Group B: 15 animals were injected with V2O5 every 3rd day during 60 days. On day 61 they were subjected to a fertility assessment test and sacrificed 5 days later. Groups C-H: 30 animals were injected with V2O5 every 3rd day and groups of 5 animals were sacrificed every 10 days after the beginning of the treatment.
- Dose selection rationale: 8.5 µg/g bw corresponds to 1/2 of the LD50 determined in the same laboratory for subchronic treatments. - Positive control:
- no positive control
Examinations
- Parental animals: Observations and examinations:
- Health indicators recorded in adult male mice were general appearance, mortality rate, and body weight (initial vs. final).
- Oestrous cyclicity (parental animals):
- not examined
- Sperm parameters (parental animals):
- Parameters examined in [P] male parental generations: testis weight, sperm count in vas deferens, sperm motility, sperm morphology
- Litter observations:
- number of litters, live and dead fetuses, and fetal body weight.
- Postmortem examinations (parental animals):
- At autopsy the testes were dissected and weighed with the aid of an analytical balance. Sperm parameters were assessed.
- Postmortem examinations (offspring):
- not examined
- Statistics:
- The results of percent of fertility, sperm motility, and abnormal sperm were analyzed using the "z"-test. The frequency of implants, resorptions, live and dead fetuses, body and testis weight, and sperm count were analyzed using the Student's t-test.
- Reproductive indices:
- incidence of pregnancy in females, number of litters, implants, resorptions, live and dead fetuses, and fetal body weight
Results and discussion
Results: P0 (first parental generation)
General toxicity (P0)
- Clinical signs:
- not examined
- Mortality:
- no mortality observed
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- The final body weight of V2O5-treated animals during 60 days was lower than controls, while differences were not observed in those animals sacrificed at days 10, 20, 30, 40, or 50 after the beginning of the treatment
- Food efficiency:
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Immunological findings:
- not examined
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Histopathological findings: non-neoplastic:
- not examined
- Histopathological findings: neoplastic:
- not examined
- Other effects:
- not examined
Reproductive function / performance (P0)
- Reproductive function: oestrous cycle:
- not examined
- Reproductive function: sperm measures:
- effects observed, treatment-related
- Description (incidence and severity):
- The sperm count diminished significantly in V2O5-treated animals during 20 days or longer. A marked reduction in sperm motility was observed with the advancement of treatment in mice treated with V2O5.
A significant increase of the percentage of morphologic abnormalities in spermatozoa obtained from vanadium-treated animals was observed after 50 and 60 days of treatment. - Reproductive performance:
- effects observed, treatment-related
- Description (incidence and severity):
- The fertility rate was significantly lower in V2O5-treated mice than in controls (10/30; 33 % vs. 34/40; 85 %, P < 0.05, "z"-test). The number of implants and pups was lower in females mating with V2O5-treated males, while the incidence of resorptions was increased in these females. The body weight of fetuses born from dams impregnated by V2O5-treated mice was lower than controls.
Details on results (P0)
Effect levels (P0)
open allclose all
- Dose descriptor:
- dose level: applied every third day during 60 days
- Effect level:
- 8 other: µg/g bw/0.3 d
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- organ weights and organ / body weight ratios
- reproductive function (sperm measures)
- reproductive performance
- Key result
- Dose descriptor:
- dose level: decrease in fertility rate, implantations, live fetuses, and fetal weight, and an increase in the number of resorptions/dam. Sperm count, motility, and morphology were impaired with the advancement of treatment
- Effect level:
- 2.7 mg/kg bw/day
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- organ weights and organ / body weight ratios
- reproductive function (sperm measures)
- reproductive performance
Target system / organ toxicity (P0)
- Critical effects observed:
- yes
- Lowest effective dose / conc.:
- 8 other: µg/g bw/0.3 d
- System:
- male reproductive system
- Organ:
- germ cells
- testes
- Treatment related:
- yes
- Dose response relationship:
- no
- Relevant for humans:
- yes
Results: P1 (second parental generation)
Effect levels (P1)
- Remarks on result:
- not measured/tested
Results: F1 generation
General toxicity (F1)
- Clinical signs:
- not examined
- Mortality / viability:
- mortality observed, treatment-related
- Description (incidence and severity):
- the number of live fetuses of V2O5-treated males is significantly reduced when compared to the controls
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- The body weight of fetuses bom from dams impregnated by V2O5 treated mice was lower than controls.
- Food efficiency:
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Sexual maturation:
- not examined
- Organ weight findings including organ / body weight ratios:
- not examined
- Gross pathological findings:
- not examined
- Histopathological findings:
- not examined
- Other effects:
- not examined
Developmental neurotoxicity (F1)
- Behaviour (functional findings):
- not examined
Developmental immunotoxicity (F1)
- Developmental immunotoxicity:
- not examined
Details on results (F1)
Effect levels (F1)
open allclose all
- Dose descriptor:
- dose level: decrease in live fetuses, and fetal weight
- Generation:
- F1
- Effect level:
- 8.5 other: µg/g bw/0.3 d
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- viability
- body weight and weight gain
- Key result
- Dose descriptor:
- dose level: decrease in live fetuses, and fetal weight
- Generation:
- F1
- Effect level:
- 2.7 mg/kg bw/day
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- viability
- body weight and weight gain
Target system / organ toxicity (F1)
- Critical effects observed:
- no
Results: F2 generation
Effect levels (F2)
- Remarks on result:
- not measured/tested
Overall reproductive toxicity
- Reproductive effects observed:
- yes
- Lowest effective dose / conc.:
- 2.7 mg/kg bw/day
- Treatment related:
- yes
- Relation to other toxic effects:
- reproductive effects in the absence of other toxic effects
- Dose response relationship:
- no
- Relevant for humans:
- yes
Any other information on results incl. tables
Table 1: Effects of Vanadium Pentoxide on Reproductive Function of CD-1 Male Mice
|
Control |
Vanadium pentoxide (8.5µg/g) |
Males (n) |
20 |
13 |
Mated females (n) |
40 |
Id |
Pregnant (n) |
34 |
10 |
Fertility (%)a |
85 |
33 |
Implantation sitesb |
10.88 ± 1 60 |
5.80 ± 1.33** |
Resorptionsb |
0.24 ± 0.42 |
2.00 ± 1.67* |
Live fetusesb |
10.53 ± 1.42 |
3.40 ± 0.49** |
Dead fetusesb |
0.12 ± 0.32 |
0.40±0.49 |
Fetal weight (mg)b |
145 ± 4.0 |
121± 7.0* |
a(Pregnant/females mated) x 100
bMean ± SD
* P < 0.05
** P<0.01
Table 2: Effects of Vanadium Pentoxide on Body and Testis Weight, Sperm Count, Sperm Motility and Sperm Morphology (Mean ± SD)
Vanadium pentoxide (8.5 µg/g) |
|||||||
|
Control |
10 days |
20 days |
30 days |
40 days |
50 days |
60 days |
Males (n) |
20 |
5 |
5 |
5 |
5 |
5 |
20 |
Initial weight(g) |
28.04 ± 0.61 |
26.74 ± 0.71 |
27 19 ±0.34 |
27.33 ± 0.51 |
28.00 ± 0.25 |
28.14 ± 0.30 |
27.44 ± 0.49 |
Final weight(g) |
31.20 ± 1.09 |
27.14 ± 0.71 |
27.00 ± 0.43 |
20.83 ± 0.66 |
27.14 ± 0.90 |
26.81 ± 1.03 |
24.64 ± 1.34* |
Testis weight (mg) |
135.90 ± 16.80 |
131.80 ± 24.30 |
134.10 ± 21.90 |
129.70 ± 23.30 |
130.10 ±20.10 |
124.00 ± 26.00* |
118.72 ± 22.33** |
Sperm count (x 106/ml) |
29.19 ± 2.43 |
23.50 ± 7.53 |
19.80 ± 5.31** |
16.67 ± 3.68** |
16.67 ± 2.68** |
19.91 ± 1.28** |
7.27 ± 2.31** |
Motility (%) |
73.10 ± 19.40 |
40.60 ± 10.10** |
35.50 ± 11.70** |
18.10 ± 13.40** |
14.20 ± 9.30** |
4.30 ± 8.80** |
4.01 ± 2.91** |
Abnormalsperm (%) |
6.40 ± 1.80 |
5.50 ± 3.90 |
3.80 ± 4.30* |
4.70 ± 5.10 |
6.90 ± 5.10 |
8.10 ± 4.10* |
10.83 ± 3.70** |
* P < 0.05
** P < 0.01
Applicant's summary and conclusion
- Conclusions:
- V2O5 treatment resulted in a decrease in fertility rate, implantations, live fetuses, and fetal weight, and an increase in the number of resorptions/dam. Sperm count, motility, and morphology were impaired with the advancement of treatment.
- Executive summary:
Effects of vanadium pentoxide (V2O5) treatment on reproductive function in male mice were investigated. V2O5 was administered intraperitoneally at a dose of 8.5 µg/g bw every three days (ca. 2.7 mg/kg bw/day) for 60 days. Reproductive functions were evaluated with fertility rate, implants, resorptions, sperm counts, motility, and morphology. V2O5 treatment resulted in a decrease in fertility rate, implantations, live fetuses, and fetal weight, and an increase in the number of resorptions/dam. Sperm count, motility, and morphology were impaired with the advancement of treatment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.