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EC number: 247-666-0 | CAS number: 26401-97-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Not reported
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Diisooctyl 2,2'-[(dioctylstannylene)bis(thio)]diacetate
- EC Number:
- 247-666-0
- EC Name:
- Diisooctyl 2,2'-[(dioctylstannylene)bis(thio)]diacetate
- Cas Number:
- 26401-97-8
- Molecular formula:
- C36-H72-O4-S2-Sn
- IUPAC Name:
- 6-methylheptyl 14-methyl-4,4-dioctyl-7-oxo-8-oxa-3,5-dithia-4-stannapentadecan-1-oate
- Reference substance name:
- Mono-n-octyltin-tri-thioglycol-acid isooctyl ester
- IUPAC Name:
- Mono-n-octyltin-tri-thioglycol-acid isooctyl ester
- Test material form:
- liquid
- Details on test material:
- - Appearance: colourless liquid
Constituent 1
Constituent 2
Method
- Target gene:
- S. typhimurium: Histidine locus
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538
- Species / strain / cell type:
- Saccharomyces cerevisiae
- Remarks:
- Strain D4
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 5.000, 1.000, 0.100, 0.010, 0.001 µL/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: deionised water or DMSO
- Methylnitrosoguanidine: Water
- 2-Nitrofluorene: DMO
- Quinacrine mustard: Water
- 2-Anthramine: DMSO
- 2-Acetylaminofluorene: DMSO
- 8-Aminoquinoline: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water/ DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- other: Methylnitrosoguanidine, Quinacrine mustard, 2-Anthramine, 2-Acetylaminofluorene, 8-Aminoquinoline
- Details on test system and experimental conditions:
- - Approximately 10^8 cells from an overnight culture of each indicator strain were added to separate test tubes containing 2.0 mL of molten agar supplemented with biotin and a trace of histidine. For non-activation tests, at least four dose levels of the test material were added to the contents of the appropriate tubes and poured over the surfaces of selective agar plates. In activation tests, a minimum of four different concentrations of the test material were added to the appropriate tubes with cells. Just prior to pouring, an aliquot of reaction mixture (0.5 mL containing the 9,000 x g liver homogenate) was added to each of the activation overlay tubes, which were then mixed, and the contents poured over the surface of a minimal agar plate and allowed to solidify.
- The plates were incubated for 48 hours at 37 °C, and scored for the number of colonies growing on each plate.
- Positive and solvent controls using both directly active positive chemicals and those that require metabolic activation were run with each assay.
- The numbers of colonies on each plate were counted and recorded on printed forms. These raw data were analysed in a computer program and reported on a printout. - Evaluation criteria:
- Evaluation Criteria for Ames Assay:
Because the procedures used to evaluate the mutagenicity of the test chemical are semi-quantitative, the criteria used to determine positive effects are inherently subjective and are based primarily on a historical data base. Most data sets are evaluated using the following criteria:
- Strains TA1535, TA1537, and TA1538
If the solvent control value is within the normal range, a chemical that produces a positive dose response over three concentrations with the lowest increase equal to twice the solvent control value is considered to be mutagenic.
- Strains TA98, TA100, and D4
If the solvent control value is within the normal range, a chemical that produces a positive dose response over three concentrations with the highest increase equal to twice the solvent control value for TA100 and two to three times the solvent control value for strains TA98 and D4 is considered to be mutagenic. For these strains, the dose response increase should start at approximately the solvent control value.
- Pattern
Because TA1535 and TA100 were both derived from the same parental strain (G-46) and because TA1538 and TA98 were both derived from the same parental strain (D3052), there is a built-in redundancy in the microbial assay. In general the two strains of a set respond to the same mutagen and such a pattern is sought. It is also anticipated that if a given strain, e.g. TA1537, responds to a mutagen in non-activation tests it will generally do so in activation tests. (The converse of this relationship is not expected.) While similar response patterns are not required for all mutagens, they can be used to enhance the reliability of an evaluation decision.
- Reproducibility
If a chemical produces a response in a single test that cannot be reproduced in one or more additional runs, the initial positive test data loses significance.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium, other: TA1535, TA1537, TA1538, TA98, TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- Saccharomyces cerevisiae
- Remarks:
- D
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TOXICITY TEST RESULTS
The test material was tested over a series of concentrations such that there was either quantitative or qualitative evidence of some chemically-induced physiological effects at the high dose level. The low dose in all cases was below a concentration that demonstrated any toxic effect. The dose range employed for the evaluation of this compound was from 0.001 to 5 µL/ plate.
WITHOUT METABOLIC ACTIVATION
The results of the tests conducted on the test material in the absence of a metabolic system were all negative.
WITH METABOLIC ACTIVATION
The results of the tests conducted on the test material in the presence of the rat liver activation system were all negative.
CONCLUSIONS
The test material did not demonstrate mutagenic activity in any of the assays conducted in this evaluation and was considered as not mutagenic under these test conditions.
Any other information on results incl. tables
Table 1: Summary of Experiment
± S9 Mix |
Concentration (µL/plate) |
Revertants/plate |
|||||
TA1535 |
TA1537 |
TA1538 |
TA98 |
TA100 |
D4 |
||
- |
Solvent 0.001 0.010 0.100 1.000 5.000 |
31 50 25 38 31 30 |
6 4 6 9 9 7 |
20 11 21 21 16 17 |
42 21 31 38 52 33 |
201 211 194 204 204 319 |
42 51 32 50 40 40 |
+ |
Solvent 0.001 0.010 0.100 1.000 5.000 |
35 38 31 14 16 23 |
14 18 21 14 12 7 |
25 17 30 25 35 21 |
51 45 42 40 49 43 |
226 224 237 243 254 272 |
44 46 47 38 45 42 |
Positive Controls |
|||||||
- |
Name |
MNNG |
QM |
NF |
NF |
MNNG |
MNNG |
Concentration (µg/plate) |
10 |
10 |
100 |
100 |
10 |
10 |
|
Revertants/plate |
935 |
448 |
834 |
939 |
>1000 |
925 |
|
+ |
Name |
ANTH |
AMQ |
AAF |
AAF |
ANTH |
DMNA |
Concentration (µg/plate) |
100 |
100 |
100 |
100 |
100 |
100(µM) |
|
Revertants/plate |
402 |
208 |
451 |
791 |
>1000 |
68 |
MNNG = Methylnitrosoguanidine
QM = Quinacrine mustard
ANTH = 2-Anthramine
NF = 2-Nitrofluorene
AAF = 2-Acetylaminofluorene
AMQ = 8-Aminoquinoline
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of the study, the test material did not demonstrate mutagenic activity in any of the assays conducted in this evaluation and was considered as not mutagenic with or without metabolic activation.
- Executive summary:
The genetic toxicity of the test material was investigated in a bacterial reverse mutation assay, broadly similar to OECD 471.
The test material was tested at 5.000, 1.000, 0.100, 0.010, 0.001 µL/plate both with and without metabolic activation, in the form of S9-mix. The following bacterial strains were used: Salmonella typhimurium tester strains TA98, TA100, TA1535, TA1537 and TA1538, and Saccharomyces cerevisiae strain D4. The overlay method of application was used.
The plates were incubated for 48 hours at 37°C, and scored for the number of colonies growing on each plate. Positive and solvent controls using both directly active positive chemicals and those that require metabolic activation were run with each assay. The numbers of colonies on each plate were counted and recorded.
The test material was tested over a series of concentrations such that there was either quantitative or qualitative evidence of some chemically-induced physiological effects at the high dose level. The low dose in all cases was below a concentration that demonstrated any toxic effect. The results of the tests conducted on the test material in the absence of a metabolic system were all negative. The results of the tests conducted on the test material in the presence of the rat liver activation system were all negative.
Under the conditions of the study, the test material did not demonstrate mutagenic activity in any of the assays conducted in this evaluation and was considered as not mutagenic with or without metabolic activation.
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