Registration Dossier
Registration Dossier
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EC number: 202-880-3 | CAS number: 100-70-9
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1979-06-12 to 1979-07-03
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- not specified
- Principles of method if other than guideline:
- no specific method information available
- GLP compliance:
- no
- Remarks:
- predates GLP
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Pyridine-2-carbonitrile
- EC Number:
- 202-880-3
- EC Name:
- Pyridine-2-carbonitrile
- Cas Number:
- 100-70-9
- Molecular formula:
- C6H4N2
- IUPAC Name:
- pyridine-2-carbonitrile
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- lot # 5257; order # 02166
Test animals
- Species:
- rat
- Strain:
- Wistar
- Remarks:
- SPF, HAN 75
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- 30 male, 30 female. Acclimatiziation period = 7 days, room temp 22-24°C, relative humidity = 42-65%, body weight at start of trial = 90 - 118 g; 1 animal/cage, food = pelletted Altromin R, period without food prior to administration = 17-18 hours
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Remarks:
- pH 7.62 suspension in water
- Details on oral exposure:
- 5 g in 100 mL
- Doses:
- 0 (control), 0.5, 0.75, 1.0, 1.25, 1.5 g/kg
- No. of animals per sex per dose:
- 10 animals/dose
- Control animals:
- yes
- Details on study design:
- observation period = 22 days
- Statistics:
- probit analysis
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 000 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 900 - <= 1 100
- Mortality:
- Mortality at 1000 mg/kg (4/10 by day 22); at 1250 (9/10 by day 22); at 1500 (10/10 by day 4)
- Clinical signs:
- other: At 1000 mg/kg doses and higher: moderate to severe apathy, lateral position, unconsciousness, high-legged walk, increase lacrimal secretion, increased salivary secretion, abdominal position.
- Gross pathology:
- Animals dying before end of observation period: suspected slight local irritation of the gastrointestinal tract (multiple petechial bleeding of the glandular part of the gastic mucosa, watery content of the small intesting) as well as supsected slight alterations of the kidneys (pale coloration).
Animals sacrificed at end of observation period: no pathological findings observed
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- LD50 of 2 -Cyanopyridine in rats = 1000 mg/kg (oral gavage)
- Executive summary:
LD50 of 2 -Cyanopyridine in rats = 1000 mg/kg (oral gavage). Meets Acute Oral Category 4 criteria.
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