Hydrogen trifluoromethoxyborate(1-), compound with methanol (1:1)

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method)

GLP compliance:

yes

Test type:

acute toxic class method

Test material

Test material information

Constituent 1

Reference substance name:

Hydrogen trifluoromethoxyborate(1-), compound with methanol (1:1)

EC Number:

220-543-9

EC Name:

Hydrogen trifluoromethoxyborate(1-), compound with methanol (1:1)

Cas Number:

2802-68-8

Molecular formula:

CH4O.CH3BF3O.H

IUPAC Name:

hydrogen trifluoro(methanolato)borate(1-) methanol (1:1)

Details on test material:

Name of the test substance used in the study report: Borfluorid-Methanol

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Young adult animals with a comparable body weight were used.
The animals were identified using cage cards and group identification by tail marking.
One animal per cage (type: stainless steel wire mesh cages, DK-III).
The animals were housed in fully air-conditioned rooms with a room temperature of 20 - 24°C and a relative humidity of 30 - 70%. The day/night rhythm was 12 h dark and 12 h light.
A standardized animal laboratory diet as well as tap water were available ad libitum.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

Reason for the vehicle: The test substance is sensitive to hydrolysis.
Form of administration: solujtion
Concentrations used were 4 and 40 g/100 ml
Administration volume: 5 ml/kg

Doses:

200 and 2000 mg/kg

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

Acclimatization period for at least 1 week.
Observation period: 14 days
Body weight determination shortly before administration, weekly thereafter and at the end of the study (before fasting period).
Recording of signs and symptoms several times on the administration day, at least once each working day for the individual animals.
A check concerning general observations and mortality was made twice each working day and one on weekends and on public holidays.
Necropsy at the last day of the observation period. Withdrawal of food at least 16 hours before killing with CO2; then necropsy with gross-pathological examination (necropsy of all animals that die as soon as possible).

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 200 - < 2 000 mg/kg bw

Mortality:

200 mg/kg: no mortalities
2000 mg/kg: all animals died within 3 hours after administration

Clinical signs:

other: 200 mg/kg: impaired general state and dyspnea 2000 mg/kg: poor general state, dyspnea, apathy, staggering, ataxia, paresis and cyanosis The surviving animals appeared normal 1 or 4 days after administration.

Gross pathology:

2000 mg/kg: hemorrhages in the mucosaof the glanduar stomach and substance discoloration of contents in the small intestines.
200 mg/kg: no pathologic findings noted

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

No mortality was observed at 200 mg/kg during the 14-day observation period, wheras all animals in the 2000 mg/kg group died shortly after administration. The data indicate that the LD50 is higher than 300 mg/kg, marking the classification threshold between categories 3 and 4. As the LD50 is clearly below 2000 mg/kg, the substance is classified into category 4.