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EC number: 224-736-9 | CAS number: 4468-02-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- LCE11031, Batch 1106900005 (Zinc Gluconate) as supplied by Givaudan Lavirotte
- Analytical monitoring:
- yes
- Details on sampling:
- At the beginning, before and after renewal and at the end of test, aliquot (approximately 20mL) was taken in each group containing the test item and in the control.
These aliquots were transferred to ICP unit to be analyzed for Zinc contents determination. - Vehicle:
- no
- Details on test solutions:
- Stock solutions were kept at 4±2°C until they were used for tha study. Stock solutions were used because they were not cloudy (without any precipitation).
On March 2011, 100 mL of stock solution 1, 2, 3, and 4 was mixed with 800 mL demineralised water and completed to 10L and 2L of stock solution 1, 2, 3, and 4 was mixed with 10L demineralised water and completed to 200L.
On April 29th, 2011, 1000mL of stock solution 1, 2, 3, and 4 was mixed with 8L demineralised water and completed to 100L. Test medium was ventilated until a value close to saturation in air and pH stabilization were obtained.
Validity criteria for test medium :
O2 dissolved > 7mg/L (90%).
pH= 7-8 ± 0.2 - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Strain: Oncorhynchus mykiss
- Source: "pisciculture", 27400 Hondouville, France
ACCLIMATION
- Acclimation period: at least 12 day before the start of the test
- Acclimation conditions: same as test
- Type of food during acclimation: trout chow.
- Feeding frequency during acclimation: Twice daily. The feeding was stopped 24 hours before the start of the test.
FEEDING DURING TEST
- Food type: trout chow - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Standard duration applied.
- Hardness:
- Not specified
- Test temperature:
- 15°C ± 2°C
- pH:
- mean of 7.87 at 96h ; 7.82 at 72h ; 7.09 at 0h
- Dissolved oxygen:
- > 60% (mean of 83.77% at 96h ; 93.72% at 72h ; 93.59% at 0h)
- Nominal and measured concentrations:
- 10 ; 20 ; 40 ; 60 ; 80 ; 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Aeration: Forced aeration to keep the dissolved oxygen concentration at or above of the saturation value at the selected temperature.
- No. of organisms per concentration: 7 fish in each concentration
OTHER TEST CONDITIONS
- pH: regular pH monitoring
- Light photoperiod cycle: 8/16 hours.
TEST CONCENTRATIONS
- Range finding study test concentration: 0.01 - 0.1 - 1 - 10 - 100 mg/L
- Test concentrations (definitive test): 10 - 20 - 40 - 60 - 80 and 100 mg/L
OTHER :
After 48 hours, news solutions were prepared and living fish were transferred into them. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11.12 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The EC-96h determined on Oncorhynchus mykiss is 11.12mg/L (confidence limits (95%): 10-10mg/L).
- Reported statistics and error estimates:
- confidence limits (95%): 10-10mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC-96h determined on Oncorhynchus mykiss is 11.12mg/L (confidence limits (95%): 10-10mg/L).
- Executive summary:
An OECD 203 study (GLP) was conducted on Oncorhynchus mykiss under semi-static conditions. This study is considered reliable. The EC-96h determined on Oncorhynchus mykiss is 11.12mg/L (confidence limits (95%): 10-10mg/L).
Reference
Description of key information
An OECD 203 study (GLP) was conducted on Oncorhynchus mykiss under semi-static conditions. This study is considered reliable. The EC-96h determined on Oncorhynchus mykiss is 11.12mg/L (confidence limits (95%): 10-10mg/L).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 11.12 mg/L
Additional information
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