Cyclohexane-1,2,4-triyltris(ethylene)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

(1984)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

EC 84/449, Nov 1989

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A suspension of 1 g/L of the test substance in synthetic freshwater was stirred for 18 hours. Then the solution was filtered and the carbon content was measured. This solution has been used as stock solution. The TOC in the stock solution was 15.8 mg/L corresponding to 17.1 mg/L test substance. The calculated test substance concentrations of 3.8 mg/l, 5.5. mg/L, 7.7 mg/L, 11.1 mg/L, and 15.4 mg/L were used in the test.
- Controls: freshwater without test substance
- Evidence of undissolved materia: not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna Straus, Strain IRCHA
- Source: breeding in the laboratory (Klon 5)
- No feeding during the test

ACCLIMATION
- Acclimation conditions (same as test or not): Breeding in M4-medium according to Elendt in 1L vessels
- Type and amount of food: Scenedesmus suspicatus, ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): parthenogenetic reproduction is warranted

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Adult daphnids were isolated 24 h before the test begin. The offspring has been transfered into test water und has been used for the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 +/- 1 °C

pH:

8.0 - 8.6 at test end

Dissolved oxygen:

8.7 - 8.8 mg O2/l at test end

Nominal and measured concentrations:

concentration of the stock solution 17.1 mg/L. Test concentrations 3.8 mg/L; 5.5 mg/L; 7.7 mg/L; 11.1 mg/L; 15.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Test vessel with 10 mL test solution
- Aeration: no
- No. of organisms per vessel: 5 daphnids per vessel (1 daphnid/ 2 mL)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Source/preparation of dilution water: synthetic freshwater
- Photoperiod: darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : (im)mobility

VEHICLE CONTROL PERFORMED: no vehicle

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 15.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 15.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 15.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The highest test substance concentration, where no immobility has been observed was 15.4 mg/L after 48 h. The lowest concentration with 100% immobility after 48 h is >15.4 mg/L. The EC50 is >15.4 mg/L after 48 h exposure. This corresponds to the highest test substance concentration tested and is the limit of water solubility found in the test. All concentrations are based on the product.

Results with reference substance (positive control):

A concentration of 0.9 mg/L potassium dichromate resulted in 5 % immobilised daphnids after 24 h. 1.9 mg/L potassium dichromate showed 95% immobilised daphnids after 24 h exposure.

Reported statistics and error estimates:

An EC50 value could not be determined by probit analysis referring to Cavalli-Sforza (1972).

Validity criteria fulfilled:

yes

Remarks:

Not more than 10% of the daphnids in the control are immobilised. The dissolved oxygen concentration is >3 mg/L at the test end.

Conclusions:

In a static toxicity test according to EC 84/449/EWG part C2 and OECD202 (1984), the acute toxicity of 1,2,4-Trivinylcyclohexane on Daphnia magna was investigated. The highest tested concentration at which no immobilisation (<=10%) was 15.4 mg/L. The EC50 after 48 h is >15.4 mg/L. The concentration of 15.4 mg/L is the highest test substance concentration tested and is the limit of water solubility found in the test. The lowest concentration with 100% immobilisation is >15.4 mg/L.

Description of key information

The acute toxicity on Daphnia magna of 1,2,4 -Trivinylcyclohexane has been examined according to EC 84/449/EWG part C2 and OECD202 (1984). The EC50 for daphnia is >15.4 mg/L after 48 h.

Additional information

The acute toxicity of 1,2,4 -Trivinylcyclohexane on Daphnia magna has been examined in a GLP conform toxicity test according to EC 84/449/EWG part C2 and OECD202 (1984). The highest tested concentration without any observed immobilisation (<=10%) has been 15.4 mg/L, which is the highest tested test substance concentration and displays the limit of water solubility found in the test. Therefore, the EC50 for daphnia is >15.4 mg/L after 48 h of exposure.

In this study, the test substance concentration has not been measured by a substance-specific analytical method. Nevertheless, the study is a guideline study, which has been performed in accordance to GLP, and the results are sufficiently documented.