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EC number: 203-380-8 | CAS number: 106-27-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-11-09 to 2015-11-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all
- Sampling method: daily taken from freshly prepared test media and from corresponding aged test media after 24h. The test item concentrations were diluted with fish dilution water with the last dilution step in a 20 mL headspace vial containing approx. 1 g sodium chloride. 100 pL methanol were added to the vials before sealing.
- Sample storage conditions before analysis: Original samples were stored at room temperature before preparation, if necessary. All prepared samples were stored at room temperature (in autosampler) before start of analysis, if necessary. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Appropriate volumes of the test item were pipetted into measuring flasks filled with 5000 mL of the dilution water. The test media were stirred on a magnetic stirrer (30 minutes, 1100 rpm, room temperature). This procedure was repeated for each renewal day. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: single brood stock
- Length at study initiation (length definition, mean, range and SD): mean: 2.34 cm
- Weight at study initiation (mean and range, SD): mean: 0.156 g
- Feeding during test: no
ACCLIMATION
- Acclimation period: at least 12 d
- Acclimation conditions (same as test or not): same
- Type and amount of food: Sera Vipan, 4% of fish body weight per feeding day
- Feeding frequency: 3 times/week (The test fish were not be fed 24 h before test start.)
- Health during acclimation (any mortality observed): no mortality observed - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 64 mg CaCO3/L
- Test temperature:
- 22.1 - 23.2 °C
- pH:
- 7.0 -7.7
- Dissolved oxygen:
- 87 - 100 % saturation
- Nominal and measured concentrations:
- Nominal: 0 (control), 0.342, 0.751, 1.65, 3.64, 8.00 mg/L
Measured (geometric mean):- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria of 13 L were used, filled with 5 L of dilution water (dimensions: 25.5/18/28 cm, depth of water: 20 cm)
- Type: semi-static (covered with aluminium foil)
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.218 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin
- Chlorine: filtered on activated charcoal and aerated for at least 24 hours to remove chlorine
- Hardness: 64 mg CaCO3/L
- pH: 7.0 - 7.7
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 9 hours/day
- Light intensity: mean: 0.81 µmol photons/m²s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, visible abnormalities (e.g. loss of equilibrium, swimming behaviour, respiratory function, pigmentation, etc.) after 2, 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study: yes
- Test concentrations: preliminary test with 0.5 mg/L (43% mortality), first definitive test as limit test with 6.8 mg/L (100% mortality)
- Results used to determine the conditions for the definitive study: yes, full test with 5 test concentrations- Reference substance (positive control):
- not required
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.47 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CI: 2.20 - 5.46 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 5.46 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities:
- Observations on body length and weight:
- Other biological observations: staggering, missing escape reflex and hyperventilation observed in highest test concentration (5.46 mg/L)
- Mortality of control: no
- Other adverse effects control: no- Sublethal observations / clinical signs:
Table 1: Cumulative mortality (%) (n=7)
Geometric mean measured concentration (mg/L)
Cumulative mortality (%) after different exposure periods (hours)
2
24
48
72
96
5.46
0
0
0
86
100
2.20
0
0
0
0
0
1.12
0
0
0
0
0
0.712
0
0
0
0
0
0.249
0
0
0
0
0
Control
0
0
0
0
0
Table 2: Environmental conditions
Exposure time (days)
Geometric mean measured concentration (mg/L)
Mean oxygen saturation (%)
pH value
Temperature (°C)
0
5.46
100
7.69
22.1
2.20
100
7.69
22.1
1.12
100
7.68
22.2
0.712
100
7.67
22.2
0.249
100
7.61
22.1
Control
100
7.51
22.1
1
5.46
94.5
7.40
23
2.20
94
7.37
22.8
1.12
94
7.37
22.9
0.712
94.5
7.35
22.9
0.249
95.5
7.19
22.8
Control
94.5
7.08
22.8
2
5.46
96.5
7.44
22.9
2.20
95
7.41
22.9
1.12
95
7.37
23
0.712
95.5
7.31
22.9
0.249
95.5
7.20
23
Control
94.5
7.06
22.7
3
5.46
95
7.42
23.1
2.20
95
7.22
23.1
1.12
96
7.19
23
0.712
96
7.16
23.1
0.249
96
7.13
23
Control
95
7.10
22.9
4
5.46
90
7.36
23.1
2.20
87
7.35
23.1
1.12
89
7.33
22.9
0.712
92
7.30
23
0.249
91
7.23
23
Control
87
7.22
22.9
- no measurements due to 100% mortality
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity of Isoamyl isovalerate on Danio rerio was tested in a GLP study according to OECD 203. The LC50 was determined to be 3.47 mg/L based on mean measured concentrations.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- The read across justification is included as attachment to section 13 in the IUCLID dataset.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.19 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: recalculated value to correct differences in molecular weight between source (MW = 172.270 g/mol) and target (MW = 158.240 g/mol).
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.47 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- other: Isoamyl Isovalerate (CAS 659-70-1)
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 5.46 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- other: Isoamyl Isovalerate (CAS 659-70-1)
- Basis for effect:
- mortality (fish)
- Conclusions:
- The toxicity of Isoamyl butyrate to fish (Danio rerio) was predicted from the source substance Isoamyl isovalerate (CAS no. 659-70-1) (see read across justification). The effect value (mortality; LC50) was predicted to be 3.47 mg/L. The recalculated value to correct differences in molecular weight between source (MW = 172.270 g/mol) and target (MW = 158.240 g/mol) was 3.19 mg/L.
Referenceopen allclose all
Description of key information
The toxicity of the test item Isoamyl butyrate to fish was predicted from the read-across substance Isoamyl isovalerate (CAS no. 659 -70 -1) (refer to the read-across justification, section 13 in the IUCLID dataset).
Both substances have a similar chemical structure and corresponding physicochemical properties. In addition they show similarity in their environmental fate such as biodegradability and their ecotoxic effects on aquatic organisms. Isoamyl isovalerate is therefore proposed suitable to predict the short-term toxicity to fish of the target substance Isoamyl butyrate.
Key study: The toxicity of the read-across substance (Isoamyl isovalerate) to fish was determined in a reliable GLP study according to OECD 203. The LC50 of 3.47 mg/L was estimated based on geometric mean measured concentrations. The recalculated value to correct differences in molecular weight between source (MW = 172.270 g/mol) and target (MW = 158.240 g/mol) was 3.19 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 3.19 mg/L
Additional information
The toxicity of the read-across substance (Isoamyl isovalerate) to fish was determined in a reliable semi-static test performed according to OECD 203 and GLP. Individuals of Danio rerio were exposed to nominal test concentrations of 0.342, 0.751, 1.65, 3.64 and 8.00 mg/L, equivalent to geometric mean measured concentrations of 0.249, 0.712, 1.12, 2.20 and 5.46 mg/L. The test concentrations were analysed by GC-FID daily. After 96 h 100% mortality was observed at 5.46 mg/L, whereas 0% mortality was recorded at 2.20 mg/L. The LC50 (96 h) was estimated at 3.47 mg/L (geometric mean measured). The recalculated value to correct differences in molecular weight between source (MW = 172.270 g/mol) and target (MW = 158.240 g/mol) was 3.19 mg/L.
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