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EC number: 274-493-8 | CAS number: 70239-77-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- of read across substance
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on toxicity study of microorganism for the test chemical :
1.The Microtox acute toxicity assay was performed by using a modified strain of Vibrio fischeri
2.To evaluate toxicity of the test material on the basis of inhibition of the light output of Vibrio fisheri - GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid
- Molecular formula: C17H14N2O8S2
- Molecular weight: 438.4356 g/mol
- Smiles notation: c1cc(cc(c1)N)C(=O)Nc2ccc(c3c2c(cc(c3)S(=O)(=O)O)O)S(=O)(=O)O
-InChl:1S/C17H14N2O8S2/c18-10-3-1-2-9(6-10)17(21)19-13-4-5-15(29(25,26)27)12-7-11(28(22,23)24)8-14(20)16(12)13/h1-8,20H,18H2,(H,19,21)(H,22,23,24)(H,25,26,27)
- Substance type: Organic
- physical state: solid - Details on sampling:
- 1. Sample storage conditions before analysis: Frozen samples were brought to room temperature, and centrifuged.
- Vehicle:
- not specified
- Test organisms (species):
- Vibrio fisheri
- Details on inoculum:
- Details on test organisms
Laboratory culture: From eight serial dilutions, the percent concentration to decrease 20% of the luminescence of amodified strain of Vibrio fischeri
(EC20) after 5 min incubation was calculated with the Microtox data analysis program
Method of cultivation: No data
Preparation of inoculum for exposure: Frozen samples were brought to room temperature, and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH. Colour correction was done at 490 nm.
Pretreatment: No data
Initial biomass concentration: No data - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 5 min
- pH:
- The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH
- Details on test conditions:
- 1.TEST SYSTEM
Test vessel: Microtox 500 Analyzer
No. of vessels per concentration (replicates): triplicate analysis.
OTHER TEST CONDITIONS
Adjustment of pH: The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH
Photoperiod:
Light intensity: Colour correction was done at 490 nm.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The percent concentration to decrease 20% of the luminescence of amodified strain of Vibrio fischeri (EC20) after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software - Reference substance (positive control):
- yes
- Remarks:
- 1. A solution of 1 g/l ZnSO4.7H2O was used as the positive control
- Duration:
- 5 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- 44.6 other: dilution
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Read across 1
- Duration:
- 5 min
- Dose descriptor:
- EC50
- Effect conc.:
- 22.1 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: inhibition of light output
- Remarks on result:
- other: Read across 2
- Results with reference substance (positive control):
- 2) EC50 refers to the concentration of compound (mg/L) required to inhibit the light output of V. fischeri by 50% after a 5 min exposure period.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic to microorganism .
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:
1.The Microtox acute toxicity assay was performed by using a modified strain ofVibrio fischeri. Frozen samples were brought to room temperature, and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70μl 1 M NaOH. Colour correction was done at 490 nm. The Microtox acute toxicity assay was performed in a Microtox 500 Analyzer on samples before and after decoloration according to the test protocols defined by the manufacturer From eight serial dilutions, the percent concentration to decrease 20% of the luminescence of amodified strain ofVibrio fischeri(EC20)after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software(1999) Azur Environmental, Newark, Del.]. A solution of 1 g/l ZnSO4·7H2O was used as the positive control and 1 g/l glucose as the negative control. Each EC20 reported is the average of triplicate analysis.
The concentration to decrease 50% of the bacterial luminescence in the Microtox acute assay (EC50) is normally reported. However, in most of these studies, the EC50 before or after decoloration was greater than 100% indicating that there was no toxicity or toxicity change. To better evaluate whether the decoloration process affected toxicity, the dilution required to decrease 20% of the bacterial luminescence relative to the control (EC20)was reported instead. The following rating was adapted from Coleman & Qureshi (1985) –
EC20:>100%=nontoxic;
>75–100%=slightly non-toxic;
>50–75%=toxic;
>25–50%=moderately toxic;
<25% very toxic.
The toxicity of 100mg/l of test material determined in terms of EC20 (% dilution) was 44.6 ± 11.6. According to the ranking scheme for Microtox assay using EC20 values, test material can be categorized under moderately toxic category.
2.Toxicity value of test materila to Vibrio fisheri was found to be EC50: 22.19+/-2.47 on the basis of inhibition of the light output after a 5 min exposure period.
Thus, based on the above summarised studies, Ammonium iodide and it’s structurally and functionally similar read across substance, it can be concluded that
4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is moderately toxic to microorganism.
Reference
Description of key information
Toxicity to microorganisms:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:
1.The Microtox acute toxicity assay was performed by using a modified strain ofVibrio fischeri. Frozen samples were brought to room temperature, and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70μl 1 M NaOH. Colour correction was done at 490 nm. The Microtox acute toxicity assay was performed in a Microtox 500 Analyzer on samples before and after decoloration according to the test protocols defined by the manufacturer From eight serial dilutions, the percent concentration to decrease 20% of the luminescence of amodified strain ofVibrio fischeri(EC20)after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software(1999) Azur Environmental, Newark, Del.]. A solution of 1 g/l ZnSO4·7H2O was used as the positive control and 1 g/l glucose as the negative control. Each EC20 reported is the average of triplicate analysis.
The concentration to decrease 50% of the bacterial luminescence in the Microtox acute assay (EC50) is normally reported. However, in most of these studies, the EC50 before or after decoloration was greater than 100% indicating that there was no toxicity or toxicity change. To better evaluate whether the decoloration process affected toxicity, the dilution required to decrease 20% of the bacterial luminescence relative to the control (EC20)was reported instead. The following rating was adapted from Coleman & Qureshi (1985) –
EC20:>100%=nontoxic;
>75–100%=slightly non-toxic;
>50–75%=toxic;
>25–50%=moderately toxic;
<25% very toxic.
The toxicity of 100mg/l of test material determined in terms of EC20 (% dilution) was 44.6 ± 11.6. According to the ranking scheme for Microtox assay using EC20 values, test material can be categorized under moderately toxic category.
2.Toxicity value of test materila to Vibrio fisheri was found to be EC50: 22.19+/-2.47 on the basis of inhibition of the light output after a 5 min exposure period.
Thus, based on the above summarised studies, Ammonium iodide and it’s structurally and functionally similar read across substance, it can be concluded that
4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is moderately toxic to microorganism.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 22 mg/L
Additional information
Toxicity to microorganisms:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:
1.The Microtox acute toxicity assay was performed by using a modified strain ofVibrio fischeri. Frozen samples were brought to room temperature, and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70μl 1 M NaOH. Colour correction was done at 490 nm. The Microtox acute toxicity assay was performed in a Microtox 500 Analyzer on samples before and after decoloration according to the test protocols defined by the manufacturer From eight serial dilutions, the percent concentration to decrease 20% of the luminescence of amodified strain ofVibrio fischeri(EC20)after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software(1999) Azur Environmental, Newark, Del.]. A solution of 1 g/l ZnSO4·7H2O was used as the positive control and 1 g/l glucose as the negative control. Each EC20 reported is the average of triplicate analysis.
The concentration to decrease 50% of the bacterial luminescence in the Microtox acute assay (EC50) is normally reported. However, in most of these studies, the EC50 before or after decoloration was greater than 100% indicating that there was no toxicity or toxicity change. To better evaluate whether the decoloration process affected toxicity, the dilution required to decrease 20% of the bacterial luminescence relative to the control (EC20)was reported instead. The following rating was adapted from Coleman & Qureshi (1985) –
EC20:>100%=nontoxic;
>75–100%=slightly non-toxic;
>50–75%=toxic;
>25–50%=moderately toxic;
<25% very toxic.
The toxicity of 100mg/l of test material determined in terms of EC20 (% dilution) was 44.6 ± 11.6. According to the ranking scheme for Microtox assay using EC20 values, test material can be categorized under moderately toxic category.
2.Toxicity value of test materila to Vibrio fisheri was found to be EC50: 22.19+/-2.47 on the basis of inhibition of the light output after a 5 min exposure period.
Thus, based on the above summarised studies, Ammonium iodide and it’s structurally and functionally similar read across substance, it can be concluded that
4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is moderately toxic to microorganism.
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