6,10-dimethylundecan-2-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

non-GLP Screening Test

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: AG guideline 92/32/EWG

GLP compliance:

no

Remarks:

Screening Test

Test material

Test material information

Constituent 1

Reference substance name:

6,10-dimethylundecan-2-one

EC Number:

216-509-8

EC Name:

6,10-dimethylundecan-2-one

Cas Number:

1604-34-8

Molecular formula:

C13H26O

IUPAC Name:

6,10-dimethylundecan-2-one

Details on test material:

- Name of test material (as cited in study report):Tetrahydrogeranylaceton
- Lot/batch No.: B 633
- Analytical purity: 97.5 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Due to the low water solubility of the test item (< 100 mg/L), the test item was mixed with M4-medium and stirred for about 20 hours followed by centrifugation at 17700 g. The resulting clear solution was then used in the test.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 hours
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19-21 °C

pH:

ca. 7 - 8

Dissolved oxygen:

> 3 mg/L

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0.01, 0.1, 1.1, 10, 100, control

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Type (delete if not applicable): open / closed
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium (according to ISO 10706) was prepared
- Alkalinity: up to pH 4.3: 0.80 - 1.00 mmol/l
- Ca/mg ratio: approx. 4 : 1
- Conductivity: 550 - 650 microS/cm



OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity:diffuse light, 2 - 7 microE/(m2*s) at a wave length of 400 - 700 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

29.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

not reported