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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1977
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1977
Report date:
1977

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
EC Number:
221-897-7
EC Name:
Anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
Cas Number:
3271-76-9
Molecular formula:
C31H15NO3
IUPAC Name:
anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
Test material form:
solid
Details on test material:
Vat Green 3

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge
Details on inoculum:
suspension of filtrate

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Reference substance (positive control):
no

Results and discussion

Effect concentrations
Key result
Duration:
3 h
Dose descriptor:
IC0
Effect conc.:
>= 156 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of heterotrophic respiration

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The 3-h IC0 to activated sludge respiration was determined at ca. 300 mg/L (corresponding to 156 mg/L act. ingr.). The substance is not toxic to bacteria and activated sludge.