Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 221-897-7 | CAS number: 3271-76-9
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1977
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
Test material
- Reference substance name:
- Anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
- EC Number:
- 221-897-7
- EC Name:
- Anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
- Cas Number:
- 3271-76-9
- Molecular formula:
- C31H15NO3
- IUPAC Name:
- anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
- Test material form:
- solid
- Details on test material:
- Vat Green 3
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- suspension of filtrate
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC0
- Effect conc.:
- >= 156 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of heterotrophic respiration
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 3-h IC0 to activated sludge respiration was determined at ca. 300 mg/L (corresponding to 156 mg/L act. ingr.). The substance is not toxic to bacteria and activated sludge.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
