Disodium 7-[[2-(acetylamino)-4-[(5-chloro-2,6-difluoro-4-pyrimidinyl)amino]phenyl]azo]naphthalene-1,3-disulphonate

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

From September 06, 1976 to September 02, 1977

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: test guideline for testing the degree of accumulation of chemical substances in the fish body

GLP compliance:

no

Test organisms (species):

Cyprinus sp.

Details on test organisms:

TEST ORGANISM
- Common name: Jcarp.
- Average body length: ca 10 cm
- Average weight: ca 28 g
- Surface sterilization: 24 hours medical bathing in10 ppm chlorotetracyclin hydrochloride acid solution under standard conditions.
- Acclimation: 14 days at 25 °C.

Route of exposure:

aqueous

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

56 d

Test temperature:

25.0 ± 2 °C

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 l glass vessel.
- Water flow: 582 l/day

PREPARATION OF FISH SAMPLE
Fish body weight and length were measured. Tissues were cutted into small pieces and suspended in 50 ml of 0.5 CH3CO2Na -HCl buffer (pH 2.8) and homogenized for 10 minutes (50 ml of water; 300 mg of proctase); pH adjustments were performed.
Residues were suspended in acetone (containing 30 water 50 ml). In order to filtrate, the residues udergo evapouration to solid state; successive passages were addition of methylalcohol (100 ml) and centrifugatin (8000 r.p.m. 5 min), 2 times.
At the end of this phase, residues were separated.
The test sample was dissolved in methylalcohol, treatment with water and ammonia and centrifuged (3000 r.p.m. 10 min).

RANGE-FINDING / PRELIMINARY STUDY
- Common name: Japanese Killifish.
- Fish weight: 0.2 g
- Test temperature: 25.0 ± 2 °C
- Results: 48 hrs TLm: 440 ppm
- Concentration setting: 400 ppm x 1/100 of 1/1000 where chosen.

Nominal and measured concentrations:

4 (concentration level 1) and 0.4 ppm (concentration level 2)

Remarks on result:

other: not determined

Details on results:

The converted concentrion of the substance resulted to be 1.06 ppm at the concentration of 4 ppm and 0.106 ppm at the concentration of 0.4 ppm.
Due to the low accumulation rate, the substance content was in traces or non-detectable.

Accumulation rate

Concentration level	2 w	4 w	6 w	8 w
At 4.0 ppm	< 5.0	< 5.0	< 5.0	< 5.0
At 0.4 ppm	< 50	< 50	< 50	< 50

Traces or not detectable

Actual measured concentration level

Conc. level	2 w	4 w	6 w	8 w
At 4.0 ppm	4.36	3.93	3.80	3.90
At 0.4 ppm	0.440	0.402	0.391	0.394

Conclusions:

The substance content was in traces or non-detectable.

Executive summary:

The bioaccumulative potential of test item was assessed in carp. The test item concentration was measured in water and in fish tissues, by HPLC method. Based on the TLm value determined in preliminary test, two concentration levels were chosen: 4.0 (concentration level 1) and 0.4 ppm (concentration level 2).

The converted concentrion of the substance resulted to be 1.06 ppm at the concentration of 4 ppm and 0.106 ppm at the concentration of 0.4 ppm. Due to the low accumulation rate, the substance content was in traces or non-detectable.

Description of key information

No bioaccumulation potential expected.

Additional information

The substance is expected to possess a low potential for bioaccumulation, based on the screening evaluation taken into account the experimental log Pow.

In addition, the bioaccumulative potential of test item was assessed in carp. The test item concentration was measured in water and in fish tissues, by HPLC method. Based on the TLm value determined in preliminary test, two concentration levels were chosen: 4.0 (concentration level 1) and 0.4 ppm (concentration level 2).

The converted concentrion of the substance resulted to be 1.06 ppm at the concentration of 4 ppm and 0.106 ppm at the concentration of 0.4 ppm. Due to the low accumulation rate, the substance content was in traces or non-detectable.