1-(2-ethylbutyl)cyclohexanecarbonyl chloride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Study performed according to: - Commission Directive 92/69/EEC, Method C.4-D of July31, 1992: Manometric Respirometry Test (EEC Publication No. L 383 A, December 1992) - OECD Guideline for Testing of Chemicals No. 301 F: "Ready Biodegradability: Manometric Respirometry Test", adopted July 17, 1992

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

and EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

yes

Remarks:

Washing of activated sludge three times instead of twice. No effect presumed as washing should be at least twice and additional washing does not affect the activated sludge.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

CAT-Acid chloride

IUPAC Name:

CAT-Acid chloride

Details on test material:

Name: CAT-Acid Chloride
Certificate of Analysis Date: March 30, 2006
Aggregate State at Room Temperature: Liquid
Colour: Brown (according to IBACON personnel)
Molecular Weight: 230.78 g/mol
Molecular Formula: C13H23C10
Calculated Theoretical Oxygen Demand: 2.496 mg/mg
Stability: In water: the test item is considered to be stable under
test conditions
Expiry Date: July 03, 2007
Storage: In original container, at 2-8 degree C, in the dark

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Duration of test (contact time):

28 h

Initial test substance concentration

Initial conc.:

ca. 102 mg/L

Based on:

other: About 102 mg/L corresponding to an oxygen demand of about 255 mg/L based on ThOD of 2.496 mg oxygen demand per mg test item.

Results and discussion

Details on results:

Under the test conditions no biodegradation of CAT-acid chloride was
observed within 28 days of incubation. According to the test guidelines,
the test item is considered not to be readily biodegradable.

Any other information on results incl. tables

Biodegradation of Test Item:

Under the test conditions no biodegradation of CAT-acid chloride was

observed within 28 days of incubation. According to the test guidelines,

the test item is considered not to be readily biodegradable.

 

Biodegradation of Sodium Benzoate:

The reference item sodium benzoate was sufficiently degraded to 63%

after 4 days, and reached a plateau higher than 100% after 11 days of incubation,

thus confirming the suitability of the used activated sludge inoculum.

 

Biodegradation of the Toxicity Control:

Inthe toxicity control containing both, the test item and the reference item

sodium benzoate, a mean of 36% biodegradation was noted within 11 days

and was at a plateau at 37% to 39% degradation within end of the experiment.

Since the degradation rate was higher than 25% after 14 days, the

test item can be assumed to be not inhibitory on the activated sludge micro

organisms.

 

Primary Degradation, Hydrolysis and Photolysis of Test Item:

The test item was found to be not stable in aqueous test solutions. Therefore,

no test item analysis could be done.

 

Adsorption of Test Item to Activated Sludge:

Due to the instability of the test item, the adsorption to activated sludge

could not be determined under the test conditions.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test item CAT-acid chloride is not readily biodegradable within the 10
day window or after 28 days of incubation. The test item is not stable under
test conditions.

Executive summary:

Title:

Ready Biodegradability of CAT-Acid Chloride in a Manometric Respirometry Test

 

Guidelines:

- Commission Directive 92/69/EEC, Method C.4-D of July31, 1992:

Manometric Respirometry Test (EEC Publication No. L 383 A, December

1992)

- OECD Guideline for Testing of Chemicals No. 301 F: "Ready Biodegradability:

Manometric Respirometry Test", adopted July 17, 1992

 

Material and Methods:

Test Item:

CAT-Acid Chloride

 

Test Species:

Activated sludge (micro organisms from a domestic waste water treatment

plant) was supplied by the sewage plant Rossdorf, Germany.

 

Test Design:

The test item CAT-acid chloride was investigated for its ready biodegradability

in a manometric respirometry test over a period of 28 days. The

biodegradation was followed by the oxygen uptake of the microorganisms

during exposure. As a reference item sodium benzoate was

tested simultaneously under the same conditions as the test item, and functioned

as a procedure control.

In addition the primary degradation, hydrolysis and photolysis were followed

by test item specific analysis after 24 hours, 120 hours and 28 days.

The adsorption of CAT-acid chloride to activated sludge was determined

by comparison of test item concentration, dissolved organic carbon (DOC)

and oxygen consumption (BOD) in the treatments after 24 hours and 28

days.

 

Endpoints :

Degradation rate of test item calculated by the oxygen consumption of the

activated sludge microorganisms after 28 days of incubation.

 

Reference Item:

Sodium benzoate

 

Test Concentration:

About 102 mg/L corresponding to an oxygen demand of about 255 mg/L

based on ThOD of 2.496 mg oxygen demand per mg test item.

 

Test Conditions:

22 degree C, darkness

 

Results:

Biodegradation of Test Item:

Under the test conditions no biodegradation of CAT-acid chloride was

observed within 28 days of incubation. According to the test guidelines,

the test item is considered not to be readily biodegradable.

 

Biodegradation of Sodium Benzoate:

The reference item sodium benzoate was sufficiently degraded to 63%

after 4 days, and reached a plateau higher than 100% after 11 days of incubation,

thus confirming the suitability of the used activated sludge inoculum.

 

Biodegradation of the Toxicity Control:

In the toxicity control containing both, the test item and the reference item

sodium benzoate, a mean of 36% biodegradation was noted within 11 days

and was at a plateau at 37% to 39% degradation within end of the experiment.

Since the degradation rate was higher than 25% after 14 days, the

test item can be assumed to be not inhibitory on the activated sludge micro

organisms.

 

Primary Degradation, Hydrolysis and Photolysis of Test Item:

The test item was found to be not stable in aqueous test solutions. Therefore,

no test item analysis could be done.

 

Adsorption of Test Item to Activated Sludge:

Due to the instability of the test item, the adsorption to activated sludge

could not be determined under the test conditions.

 

Conclusion:

The test item CAT-acid chloride is not readily biodegradable within the 10

day window or after 28 days of incubation. The test item is not stable under

test conditions.