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EC number: 288-511-7 | CAS number: 85736-99-8 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 53228.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09 October 2017 - 11 December 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- July 17, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 31 May, 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test item solution used in the test was prepared by mechanical dispersion of 0.5 g test item into 5000 mL test medium (ISO medium), in order to obtain the concentration of 100 mg/L one day before the start of the study. After approx. 15 minutes ultrasonic dispersion the test item solution was stirred for about 24 hours.
- Controls: dilution water (ISO medium) - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish (Danio rerio)
- Source: Akvárium magazin Kft. (Pasaréti Gyula), 1222 Budapest, Dévény u. 36.
- Weight at study initiation: Mean control: 0.18 g, mean exposure group (100 mg/L): 0.23 g
ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions: same as test (12 to 16 hours photoperiod daily; 21 – 25°C)
- Type of food during acclimation: fish were fed with appropriate, commercial diet for fish
- Feeding frequency during acclimation: at least three times per week, until 24 hours before the test start
- Health during acclimation: No significant mortality (less than 5 % of population) occurred in seven days before the start of the experiment.
FEEDING DURING TEST
- The fish were not fed during the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- The fish were exposued continuously and observed at approximately 24, 48, 72 and 96 hours after start of the test for signs of intoxication and mortality.
- Hardness:
- The reconstituted water (ISO medium) has an approximate theoretical total hardness of 249 mg/L (as CaCO3).
- Test temperature:
- The measured temperatures were within a range of 22.5 – 23.3°C in the test aquariums (the maximum deviation did not exceed ± 1°C).
- pH:
- The test was carried out without adjustment of pH. The measured pH values were in the range of 7.46 – 7.94 during the test.
- Dissolved oxygen:
- The dissolved oxygen concentration was in the range of 76.2– 94.8 % of the air saturation value at the temperature used. Test solutions were not aerated during the test.
- Nominal and measured concentrations:
- nominal: 100 mg/L, no measured concentrations
- Details on test conditions:
- TEST SYSTEM
- Aeration: Test solutions were not aerated during the test.
- No. of vessels per concentration (replicates): Ten fish were used as control (no replicates were used).
- No. of vessels per control (replicates): Ten fish were exposed to the test concentration (no replicates were used).
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO medium, prepared according to Annex 2 of the OECD guideline 203
OTHER TEST CONDITIONS
- Adjustment of pH: The test was carried out without adjustment of pH.
- Photoperiod: 16 hours light (artificial illumination) and 8 hours darkness
EFFECT PARAMETERS MEASURED: The fish were observed at approximately 24, 48, 72 and 96 hours after start of the test for signs of intoxication and mortality. The total length of each fish was measured at the end of the test.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test (100 mg/L)
- Range finding study
- Test concentrations: 10, 50 and 100 mg/L (nominal concentrations)
- Results used to determine the conditions for the definitive study: Yes, no dead fishes observed in any of the dose groups after 96 hours. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- mortality (fish)
- Remarks:
- or sub-lethal effects
- Remarks on result:
- other: There was no mortality or sub-lethal effects observed in 10 fish in the control group or in 10 fish exposed to a test concentration of 100 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- mortality (fish)
- Remarks:
- or sub-lethal effects
- Remarks on result:
- other: There was no mortality or sub-lethal effects observed in 10 fish in the control group or in 10 fish exposed to a test concentration of 100 mg/L.
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- mortality (fish)
- Remarks:
- mortality or sublethal effects
- Remarks on result:
- other: There was no mortality or sub-lethal effects observed in 10 fish in the control group or in 10 fish exposed to a test concentration of 100 mg/L.
- Details on results:
- - Behavioural abnormalities: none
- Observations on body length and weight: none
- Other biological observations: none
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations: precipitation was observed - Sublethal observations / clinical signs:
Results of the PreliminaryRange-Finding Test (96-h)
Nominal concentrations
[mg/L]Control
100
50
10
Number of treated animals
3
3
3
3
Number of dead animals
0
0
0
0
Cumulative mortality data in the definitive test
Test Group
Cumulative mortality
(initial population = 10 fish / test group)24h
48h
72h
96h
Control
0
0
0
0
100 mg/L
0
0
0
0
Note: No sublethal effects occurred throughout the test in the control and the treatment groups
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this 96-hour acute toxicity test on Zebrafish (Danio rerio) the test item Leuco Sulfur Brown 96 had no toxic effect on fish up to a concentration of 100 mg/L. Accordingly, the 96-h LC50 value was determined to be > 100 mg/L and the 96-h NOEC was determined to be 100 mg/L, based on nominal test concentration.
- Executive summary:
The acute toxicity of the test item to zebrafish was determined according to OECD Guideline 203. A limit test was performed using a single concentration of 100 mg test item/L. ISO medium was used as dilution water. Fish were exposed to aqueous test media containing the test item for 96 hours in a static test. Ten fish were exposed to the test concentration and ten fish were run as control (no replicates were used). Fish were observed at approximately 24, 48, 72 and 96 hours after start of the test for signs of intoxication and mortality. Measurements of pH, dissolved oxygen concentration and temperature were carried out daily.
There was no mortality or sub-lethal effects observed in fishes of the control group or in fishes exposed to a test concentration of 100 mg/L. The 96-h No Observed Effect Concentration (NOEC) was determined to be 100 mg/L and the LC50 and the LOEC values were determined to be > 100 mg/L, based on nominal test concentration.
Reference
Description of key information
In a 96-hour acute toxicity test on Zebrafish (Danio rerio) the test item Leuco Sulfur Brown 96 had no toxic effect on fish up to a concentration of 100 mg/L. Accordingly, the 96-h LC50 value was determined to be > 100 mg/L and the 96-h NOEC was determined to be 100 mg/L, based on nominal test concentration.
Key value for chemical safety assessment
Additional information
The acute toxicity of the test item to zebrafish was determined according to OECD Guideline 203. A limit test was performed using a single concentration of 100 mg test item/L. ISO medium was used as dilution water. Fish were exposed to aqueous test media containing the test item for 96 hours in a static test. Ten fish were exposed to the test concentration and ten fish were run as control (no replicates were used). Fish were observed at approximately 24, 48, 72 and 96 hours after start of the test for signs of intoxication and mortality. Measurements of pH, dissolved oxygen concentration and temperature were carried out daily.
There was no mortality or sub-lethal effects observed in fishes of the control group or in fishes exposed to a test concentration of 100 mg/L. The 96-h No Observed Effect Concentration (NOEC) was determined to be 100 mg/L and the LC50 and the LOEC values were determined to be > 100 mg/L, based on nominal test concentration.
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