Methylcyclopentadiene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well conducted GLP compliant study

Data source

Reference

Reference Type:

other: USEPA High Production Volume Information System (HPVIS)

Title:

Unnamed

Year:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

not specified

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methylcyclopentadiene

EC Number:

247-724-5

EC Name:

Methylcyclopentadiene

Cas Number:

26472-00-4

Molecular formula:

not applicable, UVCB substance

IUPAC Name:

3a,4,7,7a-tetrahydro-1H-4,7-methanoindene

Test material form:

liquid

Test animals

Species:

rat

Strain:

Fischer 344

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 5 weeks
- Weight at study initiation: Males - 173 g; females - 127 g
- Housing: Stainless steel wire mesh cages
- Diet (e.g. ad libitum): Powdered diet available ad libitum except during the 4-hour exposure period
- Water (e.g. ad libitum): Available ad libitum except during the 4-hour exposure period

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-23 deg C
- Humidity (%): 35-57%
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

clean air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Method of conditioning air: Filtration
- System of generating particulates/aerosols: Substance heated in a glass evaporator at the lowest temperature to yield a vapour of 495 ppm concentration

TEST ATMOSPHERE
- Brief description of analytical method used: GC/FID

TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: Not applicable (vapour)
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): Not applicable (vapour)

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

495 ppm

No. of animals per sex per dose:

6 males / 6 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Examined at intervals during exposure and then daily for 14 days. Body weight determined on Days 2, 6, 9 and 15
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: Clinical signs and body weights determined as stated above. Gross necropsy examination on Day 15

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC0

Effect level:

> 495 ppm

Based on:

test mat.

Exp. duration:

4 h

Mortality:

None

Clinical signs:

other: None observed

Body weight:

No changes associated with treatment

Gross pathology:

No gross lesions apparent at necropsy

Applicant's summary and conclusion

Conclusions:

Substance is not acutely toxic to rats following 4-hours inhalation exposue to a vapour concentration of 495 ppm

Executive summary:

Substance is not acutely toxic to rats following 4-hours inhalation exposue to a vapour concentration of 495 ppm