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Diss Factsheets
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EC number: 232-313-5 | CAS number: 8002-53-7 Wax obtained by extraction of lignite.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study following OECD test guideline 405 under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- in vitro HET-CAM Test was applied
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Montan wax
- EC Number:
- 232-313-5
- EC Name:
- Montan wax
- Cas Number:
- 8002-53-7
- Molecular formula:
- As montan wax is a UVCB, a molecular formular cannot be specified.
- IUPAC Name:
- Montan wax
Constituent 1
Test animals / tissue source
- Species:
- other: not applicable as invitro HET-CAM was used
- Strain:
- not specified
- Details on test animals or tissues and environmental conditions:
- not applicable
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: not applicable, but in vitro a negative and two positive controls were used
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 300ml (i.e. approx. 167.5 mg) - Duration of treatment / exposure:
- 300 seconds
- Observation period (in vivo):
- 300 seconds
- Number of animals or in vitro replicates:
- not applicable as in vitro test was used (in the HET-CAM six eggs were used for the test substance and three eggs per control)
- Details on study design:
- SCORING SYSTEM: Irritation score (IS) based on time until haemorrhage, time until lysis and time until coagulation
IS=((301-seconds until haemorrhage)/300)*5 + ((301-seconds until lysis)/300)*7 + ((301-seconds until coagulation)/300)*9
Results and discussion
In vivo
Results
- Irritation parameter:
- other: irritation score
- Basis:
- mean
- Time point:
- other: 300 seconds
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- other: not applicable
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: other: classification based on IS
- Conclusions:
- Based on the results of an in vitro HET-CAM test following OECD test guideline 405 under GLP, the test substance is considered not irritating to the eye.
- Executive summary:
An in vitro HET-CAM study was performed to assess the irritating potential of the test substance by detection
of damages in blood vessels under the chorionallantoic membrane of incubated chicken eggs (nine days old). Observation time was 5 minutes at room temperature.
Physiological sodium chloride solution was used as negative control, sodium dodecyl sulphate (1% solution) and sodium hydroxide (0.1-N solution) were used as positive controls.
The positive controls induced a severe irritation on the blood vessels. The negative control showed no irritation, the irritation score for the positive controls lay within the demanded range.
The test item was tested pure. A mean irritation score of 0.0 was calculated, corresponding to a classification as not irritant.
No observations were made which might cause doubts concerning the validity of the study outcome.
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