Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 807-113-1 | CAS number: 3709-71-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study was conducted in compliance with OECD GLP (1992) regulations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- yes
- Remarks:
- Relative humidity of the room was 50-90%. N-hexane was used rather than dichloromethane to absorb the exposure atmosphere after finding incomplete absorption with dichloromethane.
- Principles of method if other than guideline:
- Deviations: Relative humidity of the room was 50-90%. N-hexane was used rather than dichloromethane to absorb the exposure atmosphere after finding incomplete absorption with dichloromethane. As a result, exposure of animals in group one with dichloromethane use were not reported. Only the results of the exposure of Group 2 utilizing n-hexane are contained in the report.
- GLP compliance:
- yes
- Test type:
- fixed concentration procedure
- Limit test:
- yes
Test material
- Reference substance name:
- T-6329
- IUPAC Name:
- T-6329
- Test material form:
- other: Liquid
- Details on test material:
- - Name of test material (as cited in study report): T-6329
- Substance type: Mono-constituent
- Physical state: Liquid
- Analytical purity: >93%
- Purity test date: No data
- Lot/batch No.: L12596
- Expiration date of the lot/batch: June 1996
- Storage condition of test material: At room temperature, protected from light
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: BRL, Biological Research Laboratories Ltd
- Age at study initiation: Males: 7 weeks, Females: 11 weeks
- Weight at study initiation: Males: 181.4-194.7 g, Females: 185.0-198.2 g
- Fasting period before study: None
- Housing: Group housed by sex in Makrolon typ IV cages with standard softwood bedding
- Diet (e.g. ad libitum): Kilba 343 rat maintenance diet Batch number 89/95 ad libitum
- Water (e.g. ad libitum): Tap water ad libitum
- Acclimation period: Six days under laboratory conditions
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-25 C
- Humidity (%): 50-90%
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: 21 July 1995 To:10 August 1995
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Nose-only, flow-past exposure using the Sachsse et al. (1973, 1976) method.
- Method of holding animals in test chamber: Restraint tubes
- Source and rate of air: Clean compressed air was utilized at a generator airflow of 10 L/min and a dilution airflow rate of 6 L/min
- Method of conditioning air:
- System of generating particulates/aerosols: Test article vapor was generated using a nebulizer. The vapor was diluted with filtered compressed air to achieve the required exposure concentrations. The generated vapor was passed through a HEPA capsule filter to remove any aerosol droplets present.
- Treatment of exhaust air: No data
- Temperature, humidity, pressure in air chamber: Temp: 22.6-23.0 C, Humidity: 8.1-11.7 % relative humidity
TEST ATMOSPHERE
- Brief description of analytical method used: Gas chromatography
- Samples taken from breathing zone: yes - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- ca. 4 h
- Concentrations:
- 21.69 mg/L
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations were made once per hour during exposure and daily thereafter. Body weights were recorded on Days 1, 4, 8, and 15.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 21.69 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- Two male and two female animals were found dead on test days 4 and 5.
- Clinical signs:
- other: During exposure, no clinical signs were detected in any animals. On test day 4, the following clinical signs were observed: tachypnea in 1 male and 1 female; frightened behavior in 1 male and tremor in 1 male. On test day 5, two of the affected animals
- Body weight:
- All surviving animals, with the exception of 1 male and 1 female, gained weight consistently during the observation period. A single male and single female lost weight during the first week following exposure, but gained weight to exceed their pre-exposure weight during the second week following exposure.
- Gross pathology:
- No macroscopic findings were noted in the animals that survived through the observation period. In the animals that died during the observation period the following was observed: lungs incompletely collapsed, dark red discoloration (2/2). The female that died had signs of cannibalism with the larynx, tongue and thyroid gland missing.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Based on the results of the study, the 4-hour rat inhalation LC50 of the test article is greater than 21.69 mg/L, vapor.
- Executive summary:
To evaluate the acute inhalation toxicity of the test article (liquid, purity >93%, molecular weight 300 g/mol, vapor pressure 235 mmHg at 25 C,Batch No. L12596), the test material was administered to Wistar rats for a single continuous 4-hour period. The study was performed under OECD GLP (1992) conditions. The study method was based on OECD Guideline 403 (1981) and EC Directive 92/69/EEC, Part B.2 (1992). Rats (5/sex) were exposed by nose-only to 21.69 +/- 1.084 mg/L (vapor) MTDID 948, dissolved in n-hexane, for 4 hours. Following exposure, the animals were observed for 14 days. After the observation period, the animals were anesthetized by i.p. injection of sodium pentobarbital (at least 320 mg/kg-bw) and were euthanized by exsanguination. Body weights and clinical observations were recorded. Gross necropsies were performed. During the exposure period, no clinical signs were detected in any animals. One male and one female were found dead on test day 4. On test day 4, the following clinical signs were observed in the surviving animals: tachypnea (1 male/3 females), hunched posture (0/1), ruffled fur (2/1) frightened behavior (1/0), and tremor (1/0). On test day 5, one male and one female were found dead. The remaining animals had returned to normal on test day 5. No clinical signs were detected in the remainder of the observation period. All surviving animals gained weight consistently during the observation period, with the exception of one male and one female, which lost weight during the first week post exposure but regained weight to exceed their pre-exposure weight during the second week post exposure. Macroscopic findings were only detected in the animals that died during the observation period. The findings were: lungs incompletely collapsed, dark red discoloration (2 males/2 females); cannibalism, larynx, tongue, and thyroid gland, missing (0 males/1 female). Based on the results of this study, the 4-hour rat inhalation LC50 of the test article is greater than 21.69 mg/L (vapor).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.