N-benzyl-N-ethylaniline

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from Advisory Board for Environmentally Relevant Existing Substances (BUA)

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance N-benzyl-N-ethylaniline.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: N-benzyl-N-ethylaniline
- Molecular formula: C15H17N
- Molecular weight: 211.306 g/mol
- Smiles notation: N(c1ccccc1)(Cc1ccccc1)CC
- InChl: 1S/C15H17N/c1-2-16(15-11-7-4-8-12-15)13-14-9-5-3-6-10-14/h3-12H,2,13H2,1H3
- Substance type: Organic
- Physical state: Liquid

Oxygen conditions:

not specified

Inoculum or test system:

not specified

Duration of test (contact time):

28 d

Based on:

not specified

Parameter followed for biodegradation estimation:

other: BOD

Key result

Parameter:

other: BOD

Value:

0

Sampling time:

28 d

Remarks on result:

other: Other details not known

Details on results:

Test substance undergoes 0% degradation by BOD parameter in 28 days.

Validity criteria fulfilled:

not specified

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The percentage degradation of test substance N-benzyl-N-ethylaniline was determined to be 0% by BOD parameter in 28 days. Thus, based on percentage degradation, N-benzyl-N-ethylaniline is considered to be not readily biodegradable in water.

Executive summary:

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance N-benzyl-N-ethylaniline (CAS no.92-59-1).The percentage degradation of test substance N-benzyl-N-ethylaniline was determined to be 0% by BOD parameter in 28 days. Thus, based on percentage degradation, N-benzyl-N-ethylaniline is considered to be not readily biodegradable in nature.

Description of key information

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance N-benzyl-N-ethylaniline (CAS no.92-59-1) (Advisory Board for Environmentally Relevant Existing Substances (BUA), 2000).The percentage degradation of test substance N-benzyl-N-ethylaniline was determined to be 0% by BOD parameter in 28 days. Thus, based on percentage degradation, N-benzyl-N-ethylaniline is considered to be not readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

Various experimental study for the target compound N-benzyl-N-ethylaniline (CAS No. 92-59-1) and supporting weight of evidence studies for its read across substance were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental study from secondary source (Advisory Board for Environmentally Relevant Existing Substances (BUA), 2000), biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of test substance N-benzyl-N-ethylaniline (CAS no.92-59-1).The percentage degradation of test substance N-benzyl-N-ethylaniline was determined to be 0% by BOD parameter in 28 days. Thus, based on percentage degradation, N-benzyl-N-ethylaniline is considered to be not readily biodegradable in nature.

 

Another biodegradation study was conducted for evaluating the percentage biodegradability of test substance N-benzyl-N-ethylaniline (CAS no.92 -59 -1) (B.C.J. Zoeteman et. al; 1980). Rhine river surface water was used as a test inoculum obtained from Lobith or Gorinchem and the Rhine river surface water has a depth of approx. of 4 -5 meters. Initial test substance conc. used in the study was 0.001 mg/l, respectively. A careful sampling of Rhine water at Lobith was carried out for quantification of a wide range of chemicals in the water by means of GC-MS analyses of concentrates prepared by closed-loop gasstripping and XAD adsorption techniques. Assuming that reduction of the concentration of the chemicals takes place according to a first order overall process, the corresponding reaction constant and half-life was estimated from the observed drop in concentration during the known retention period. The percentage degradation of test substance N-benzyl-N-ethylaniline was determined to be 50% in 0.1 days (2.4 hrs). Thus, based on percentage degradation, N-benzyl-N-ethylaniline is considered to be readily biodegradable in nature.

 

In a supporting weight of evidence study from peer reviewed journal (H. Heukelekian and M. C. Rand, 1955) for the read across chemical N,N-diethylaniline (CAS no. 91-66-7), biodegradation experiment was conducted for 5 days for evaluating the percentage biodegradability of read across substance N, N-diethylaniline using standard dilution method at a temperature of 20°C. Sewage was used as a test inoculum. The 5 day BOD value of chemical N, N-diethylaniline was determined to be 0 g/g. Thus, based on this value, N, N-diethylaniline is considered to be not readily biodegradable in nature.

 

Additional biodegradation study was conducted for 5 days for evaluating the percentage biodegradability of read across substance N, N-diethylaniline (CAS no. 91-66-7) (HSDB, 2017). Sewage sludge was used as a test inoculum. The percentage degradation of substance N, N-diethylaniline was determined to be 5% by BOD parameter in 5 days. Thus, based on percentage degradation, N, N-diethylaniline is considered to be not readily biodegradable in nature.

 

For the same read across chemical N,N-diethylaniline (CAS no. 91-66-7) from authoritative database (J-CHECK and Envichem, 2017), biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of read across substance N, N-diethylaniline. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of substance N, N-diethylaniline was determined to be 0% by BOD parameter in 14 days. Thus, based on percentage degradation, N, N-diethylaniline is considered to be not readily biodegradable in nature.

 

In a supporting weight of evidence study from authoritative database (J-CHECK, 2017) for the read across chemical N, N-dimethylaniline (CAS no. 121-69-7),biodegradation experiment was conducted for 14 days for evaluating the percentage biodegradability of read across substance N, N-dimethylaniline. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of substance N, N-dimethylaniline was determined to be1.9, 12.3 and 0% by BOD, TOC removal and GC parameter in 14 days. Thus, based on percentage degradation, N, N-dimethylaniline is considered to be not readily biodegradable in nature.

 

Although one study from peer reviewed journal ((B.C.J. Zoeteman et. al; 1980) indicate that the test chemical N-benzyl-N-ethylaniline is readily biodegradable in nature, but based on the secondary source (2000) in which the study has been carried out according to standard procedure and on the basis of above results for its read across substance (from authoritative database HSDB, J-CHECK, Envichem and peer reviewed journals), it can be concluded that the test substance N-benzyl-N-ethylaniline can be expected to be not readily biodegradable in nature.