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Diss Factsheets
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EC number: 205-132-4 | CAS number: 134-20-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- To determine the toxicity of test chemical to microoganisms for 24 hours by Liquid Culture method.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Details on sampling:
- - Samples were prepared as 10% (w/v) solutions in 95% (v/v) ethanol.
- In all experiments appropriate solvent controls were included. Antimicrobial chemicals included as controls were: TCC, Monsanto; Irgasan DP 300, Clba-Geigy; and hexachlorophene.
Stock cultures:
- Stock cultures were maintained on agar slants composed of tryptone (Difco), 0.3%; yeast extract (Difco), 0.3%; glucose, 0.9 K2HPO 4, 0.1%, and agar, 1% (referred to as TGY agar). Slants for the diphtheroid strain were supplemented with 0.5% Tween 80 (ICI America, Inc.).
Test media:
- TGY medium was used without the agar, and the pH was not adjusted (pH 7.0) in order to optimize growth of all the test organisms.
- In this case the diphtheroid medium was supplemented with 0.1% Tween 80. - Vehicle:
- yes
- Test organisms (species):
- other: Staphylococcus aureus, Escherichia coli, Candida albicans and diphtheroid
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- pH:
- 7.0
- Nominal and measured concentrations:
- Nominal concentrations: 0.1, 1.0, 10, 100, and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: broth cultures (18 x 150 mm tubes; 10.0 ml/tube). - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 24 h
- Dose descriptor:
- other: MIC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: for S. aureus and E.coli
- Duration:
- 24 h
- Dose descriptor:
- other: MIC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: for C. albicans and Diphtheroid
- Details on results:
- - Control antimicrobials were tested in the range 0.02 to 200 ppm depending on the chemical and the organism.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The Minimum Inhibitory Concentration of the test chemical for 24 h on Staphylococcus aureus, Escherichia coli and Candida albicans, diphtheroid was determined to be >1000mg/L and 1000mg/L, respectively.
- Executive summary:
A study was conducted to determine the minimum inhibition concentration on microorganisms for 24h. Organisms used for the study were Staphylococcus aureus, Escherichia coli, Candida albicans and diphtheroid, which were obtained from Monmouth Medical Center, Long Branch, New Jersey and the Department of Dermatology, University of Pennsylvania, respectively. Nominal concentrations used were 0.1, 1.0, 10, 100, and 1000 mg/L. MIC was determined by Liquid Culture method in which TGY medium was used without the agar, while the pH was 7.0. Thus, The Minimum Inhibitory Concentration of the test chemical for 24 h on Staphylococcus aureus, Escherichia coli and Candida albicans, diphtheroid was determined to be >1000mg/L and 1000mg/L, respectively.
Reference
Description of key information
A study was conducted to determine the
minimum inhibition concentration on microorganisms for 24h. Organisms
used for the study were Staphylococcus aureus, Escherichia coli, Candida
albicans and diphtheroid, which were obtained from Monmouth Medical
Center, Long Branch, New Jersey and the Department of Dermatology,
University of Pennsylvania, respectively. Nominal concentrations used
were 0.1, 1.0, 10, 100, and 1000 mg/L. MIC was determined by Liquid
Culture method in which TGY medium was used without the agar, while the
pH was 7.0. Thus, The Minimum Inhibitory Concentration of the test
chemical for 24 h on Staphylococcus aureus, Escherichia coli and Candida
albicans, diphtheroid was determined to be >1000mg/L and 1000mg/L,
respectively.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
Based on the experimental data for the target as well as read-across analogues which are extracted by using mechanistic approach and functionally and structurally similar to the target chemical toxicity of test chemical was determined on the basis of growth inhibition of microorganisms. The studies are summarized as below:
A study was conducted to determine the minimum inhibition concentration on microorganisms for 24h. Organisms used for the study were Staphylococcus aureus, Escherichia coli, Candida albicans and diphtheroid, which were obtained from Monmouth Medical Center, Long Branch, New Jersey and the Department of Dermatology, University of Pennsylvania, respectively. Nominal concentrations used were 0.1, 1.0, 10, 100, and 1000 mg/L. MIC was determined by Liquid Culture method in which TGY medium was used without the agar, while the pH was 7.0. Thus, The Minimum Inhibitory Concentration of the test chemical for 24 h on Staphylococcus aureus, Escherichia coli and Candida albicans, diphtheroid was determined to be >1000mg/L and 1000mg/L, respectively.
A study was conducted to determine the toxicity of test chemical on microorganisms for 24h. Organisms used for the study were Staphylococcus aureus, Escherichia coli, Candida albicans and diphtheroid, which were obtained from Monmouth Medical Center, Long Branch, New Jersey and the Department of Dermatology, University of Pennsylvania, respectively. Petri Plate-Paper Disc method was used, wherein, TGY agar was used. The Paper discs of were soaked with 20µl of the 10% test solutions. Thus, the zone diameter of the test chemical for 24 h on Escherichia coli, Staphylococcus aureus, Candida albicans and diphtheroid by Petri Plate-Paper Disc method was determined to be 14mm, 12mm, 16mm and 0mm respectively.
A study was conducted to determine the toxicity of test chemical on Pseudomonas putida for 16h. Study was performed according to ISO 10712 (Water quality – Pseudomonas putida growth inhibition test (Pseudomonas cell multiplication inhibition test. After the exposure of test chemical for 16 h, the EC50 and EC10 value were determined to be 380mg/L and 140mg/L, respectively
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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