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EC number: 246-426-2 | CAS number: 24717-85-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 Aug -20 Oct 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 2008/Commission Regulation (EC) No 440/2008 of 30 May 2008 laying down test methods
pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the
Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals
(REACH), C.2, Acute Toxicity for Daphnia - Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Swiss federal office of public health; consumer protection directorate; notification authority for chemicals; CH-3003 Bern
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: samples were taken at the start and at the end of the two 24-hour test medium renewal periods
- Sample storage conditions before analysis: deep-frozen (at about -20 °C) - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Differential loading: highest test concentration (with a loading rate of 100 mg/L) intensive stirring for three hours in the dark in a closed stirring vessel; after stirring the solution was filtered through a 0.45 μm membrane filter (Whatman, NC45 , pre-conditioned with 200 mL filtrate). The undiluted filtrate was used as highest test concentration. It was further diluted with test water to prepare the test media with the lower test concentrations of the dilutions 1:2, 1:4, 1:8 and 1:16.
- Controls: yes
- Others:
At the start of the test (Day 0) and at the test medium renewal (Day 1) the highest test concentration (undiluted filtrate) was prepared with a loading rate of 100 mg/L considering the relative density of the test item of 0.90. The test item was mixed into test water by intensive stirring for three hours in the dark in a closed stirring vessel to dissolve a maximum of test item in test water. The stirring time was based on the stirring pre-experiment which showed, that the maximum amount of dissolved test item was reached after this stirring time. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna Straus
- Age of organisms at test start: 6-24 hours
- Feeding during test: no
- Food type: stock culture was fed with algal suspension of the green algae Desmodesmus subspicatus or a mixture of this algal suspension and a commercial fish diet
- Frequency: stock culture was fed three times a week
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L CaCO3; 2.5 mmol/L
- Test temperature:
- 20 and 21 °C.
- pH:
- 7.7 to 7.9 (control)
7.5 to 7.9 (test concentrations) - Dissolved oxygen:
- 8.5 to 8.8 (control)
8.3 to 8.8 (test concentrations) - Nominal and measured concentrations:
- control; 1:16 (not analysed since below the NOEC); 1:8 (not analysed since below the NOEC); 1:4 (0.051 mg/L); 1:2 (0.11 mg/L) and undiluted filtrate (0.24 mg/L) (mean measured concentration of the test item)
- Details on test conditions:
- TEST SYSTEM
Test vessel:
- Type: closed
- Material, size, headspace, fill volume: glass vessels completely filled (without headspace) with 60 mL, tightly sealed with glass stoppers
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Other: for each daphnid 12 mL (60 mL per replicate)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted test water according to ISO was used (test water was aerated prior to test start until oxygen saturation was achieved)
- Ca/mg ratio: 4:1
- Na/K ratio: 10:1
- Alkalinity: 0.8 mmol/L
- Culture medium different from test medium: no
- Intervals of water quality measurement: the pH values, dissolved oxygen concentrations and water temperature were determined in each treatment at test start and end of the test medium renewal periods
OTHER TEST CONDITIONS
- Photoperiod: 16-hour light to 8-hour dark cycle with a 30-minute transition period
- Light intensity: between 15 and 18 μmol m-2 s-1
EFFECT PARAMETERS MEASURED: immobility of the daphnids was determined by visual inspection after 24 and 48 hours of exposure
RANGE-FINDING STUDY
- Test concentrations: control; 1:20; 1:5 and undiluted filtrate (100 mg/L)
- Results used to determine the conditions for the definitive study: Based on the results of the pre-experiments the following test concentrations for the main test were chosen - Reference substance (positive control):
- yes
- Remarks:
- Potassium chlorid
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits
- Remarks:
- 0.085 - 0.12 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.051 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 0.24 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.051 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: no effect was oberved
- Results with reference substance (positive control):
- - Dose-response test: yes
- ECx: 24-hour EC50 1.0 mg/L
- Other: For evaluation of the quality of the daphnia clone and the experimental conditions, potassium dichromate is tested as a positive control twice a year. The result of the latest positive control test in October 2016 (IES Laboratories Study 20160278) showed that the sensitivity of the test organisms was within the range given by the guideline (24-hour EC50: 0.60-2.1 mg/L). - Reported statistics and error estimates:
- The 48-hour EC50 was calculated by Probit Analysis using linear maximum likelihood regression. Statistical analysis was performed using ToxRat Professional®.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item had acute toxic effects on Daphnia magna in a 48-hour semi-static test. The 48-hour EC50 was calculated to be 0.10 mg/L (arithm. mean measured concentration).
Reference
Table 1: Effect of test item on the mobility of Daphnia magna
Treatment /Dilution |
Mean measured concentration |
No. of daphnids tested |
Immobilized daphnids after 24 hours |
Immobilized daphnids after 48 hours |
||
|
[mg/L] |
|
No. |
[%] |
No. |
[%] |
Control |
--- |
20 |
0 |
0 |
0 |
0 |
Dilution 1:16 |
n.a. |
20 |
0 |
0 |
0 |
0 |
Dilution 1:8 |
n.a. |
20 |
0 |
0 |
0 |
0 |
Dilution 1:4 |
0.051 |
20 |
0 |
0 |
0 |
0 |
Dilution 1:2 |
0.11 |
20 |
0 |
0 |
13 |
65 |
Undilutedfiltrate* |
0.24 |
20 |
14 |
70 |
20 |
100 |
n.a.: Not analyzed since below the NOEC determined in this test
*: Undiluted filtrate of an equilibrated test item emulsion with a loading rate of 100 mg/L
Validity criteria
The test is considered to be valid, as in the control no daphnids showed immobilization or other signs of disease or stress. Furthermore, the dissolved oxygen concentration at the end of the test was ≥3 mg/L in the control and test vessels.
Analytical results:
At the end of the two renewal periods, the test item concentrations were in the range of 70 to 85% of the initially measured values, demonstrating a slightly decrease of the test item concentrations during the 24-hour renewal periods. The mean measured test item concentrations over the test period of 48 hours were calculated as the arithmetic mean of the two geometric means which were determined from the test item concentrations measured at the start and end of each of the two test medium renewal periods.
Table 3: Results for Test Samples
Sampling Day / Age of Sample |
Dilution Factor of Filtrate of Loading Rate 100 mg Test Item /L |
Measured Concentration of Test Item x |
Sample Preparation Factor F |
Determined Concentration of Test Item c |
% of Initially Measured Concentration |
[d/h] |
|
[mg/L] |
|
[mg/L] |
[%] |
0/0 |
Control |
n.d. |
1.67 |
<LOQ |
- |
(fresh) |
Dilution 1:4 |
0.0335 |
1.67 |
0.0559 |
- |
|
Dilution 1:2 |
0.0731 |
1.67 |
0.122 |
- |
|
Undiluted Filtrate |
0.152 |
1.67 |
0.254 |
- |
1/24 |
Control |
n.d. |
1.67 |
<LOQ |
n.a. |
(aged) |
Dilution 1:4 |
0.0257 |
1.67 |
0.0428 |
77 |
|
Dilution 1:2 |
0.0541 |
1.67 |
0.0901 |
74 |
|
Undiluted Filtrate |
0.120 |
1.67 |
0.200 |
79 |
1/0 |
Control |
n.d. |
1.67 |
<LOQ |
- |
(fresh) |
Dilution 1:4 |
0.0383 |
1.67 |
0.0639 |
- |
|
Dilution 1:2 |
0.0823 |
1.67 |
0.137 |
- |
|
Undiluted Filtrate |
0.168 |
1.67 |
0.279 |
- |
2/24 |
Control |
n.d. |
1.67 |
<LOQ |
n.a. |
(aged) |
Dilution 1:4 |
0.0269 |
1.67 |
0.0449 |
70 |
|
Dilution 1:2 |
0.0620 |
1.67 |
0.103 |
75 |
|
Undiluted Filtrate |
0.142 |
1.67 |
0.237 |
85 |
LOQ: 0.017 mg/L
n.d. = not detected
n.a. = not applicable
Description of key information
EC50 (48h) = 0.10 mg/L (Daphnia magna; OECD 202; arithmetic mean)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.1 mg/L
Additional information
There is one GLP guideline study available, which assessed the effects of the substance towards aquatic invertebrates (Daphnia magna) according to the OECD guideline 202. As the test item is a volatile substance, the test was performed using glass tubes completely filled with test medium that were tightly sealed with glass stoppers to avoid losses of test item by evaporation. A semi-static test design with a test medium renewal after 24 hours was used. Due to the low water solubility of the test item, the test media were prepared as saturated solution with a loading rate of 100 mg/L. Therefore 100 mg/L test item was mixed in test water, and was intensively stirred for 3 hours. After stirring the emulsion was filtered through a 0.45 μm membrane filter. The undiluted filtrate and the dilutions 1:2, 1:4, 1:8, and 1:16 of the filtrate were used as test media. The concentration range was previously established in a preliminary range-finding test. The loading rates of the test item were analytically verified by HPLC-UV. At the end of the two renewal periods, the test item concentration were in a range of 70 to 80% of the initially measured values, demonstrating a slightly decrease of the test item concentrations during the 24 -hour renewal periods. The measured test item concentrations were calculated as the arithmetic mean of the two geometric means. The EC50 (48 h) value was 0.10 mg/L (based on the arithmetic mean).
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