Isobutyronitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study was done prior to GLP and testing guidelines. Thus, the report is lacking in detail, but was done at a highly reputable laboratory to the standards of the time.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1961

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

The study was conducted pre-guideline, however, the format was similar to an OECD 401-type of study. Groups of rats were treated with a range of doses of the test article followed by 14 days of observation.

GLP compliance:

no

Test type:

other:

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

iso-Butyronitrile

IUPAC Name:

iso-Butyronitrile

Constituent 2

Reference substance name:

Isobutyronitrile

EC Number:

201-147-5

EC Name:

Isobutyronitrile

Cas Number:

78-82-0

Molecular formula:

C4H7N

IUPAC Name:

2-methylpropanenitrile

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

not specified

Details on test animals or test system and environmental conditions:

None provided

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

Rats were treated with a 10% aqueous solution of iso-Butyronitrile or a stock solution. The report is not clear in the results regarding which one is reported, so as a worst-case, we would assume that the results are for the 10% solution.

Doses:

10-3200 mg/kg

No. of animals per sex per dose:

Not given

Control animals:

no

Details on study design:

Animals were administered a single dose of iso-butyronitrile by oral gavage, followed by a 14-day observation period.

Results and discussion

Effect levels

Dose descriptor:

approximate LD50

Effect level:

50 mg/kg bw

Clinical signs:

other: Moderate to very weak, rapid and labored respiration, prostration, very severe vasodilation, especially in lower doses.

Applicant's summary and conclusion

Interpretation of results:

highly toxic

Remarks:

Migrated information Criteria used for interpretation of results: US CPSC / US OSHA

Conclusions:

The observed LD50 was 50 mg/kg BW

Executive summary:

Rats were treated with iso-butryonitrile by oral gavage with either stock test article or 10% in water. The report was not clear regarding which type of dose was reported, so the 10% solution is assumed as a worst case measure. The observed LD50 was 50 mg/kg BW