3-diethylaminopropiononitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-diethylaminopropiononitrile

EC Number:

226-323-9

EC Name:

3-diethylaminopropiononitrile

Cas Number:

5351-04-2

Molecular formula:

C7H14N2

IUPAC Name:

3-(diethylamino)propanenitrile

Details on test material:

- Name of test material (as cited in study report): N'N-Diethylaminopropionitril
- Physical state: liquid
- Analytical purity: 99.6%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: For the preparation of the stock solution the test substance was stirred for about 10 min at 22 ± 1 C in M4- Medium. The nominal concentration of the stock solution was 100 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: STRAUS
- Source: The clone of Daphnia magna STRAUS used was suppiied by Institut National de Recherche Chimique Appliquäe, France, in 1978. The daphnids are cultured under ständard conditions in the laboratory.
- Age at study initiation (mean and range, SD): < 24h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) with fIat bottom (Nominal volume 20 mL)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Nominal concentrations verified analytically