Isopropylcyclohexane

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983-02-21 to 1983-03-07

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

1981

Deviations:

no

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Isopropylcyclohexane

EC Number:

211-792-4

EC Name:

Isopropylcyclohexane

Cas Number:

696-29-7

Molecular formula:

C9H18

IUPAC Name:

(propan-2-yl)cyclohexane

Test material form:

liquid

Details on test material:

purity 99.8%

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

- Strain: Wistar (Bor: WISW (SPF TNO))
- Source: Winkelmann, Borchen (Germany)
- Weight at study initiation: females 122 g, males 139 g (mean) Environmental conditions:
- Feed: R 10 complete feed for rats (Ssniff, Soest; Germany)
- Water: tap water ad libitum
- Room temperature: 20°C (+/- 1°C)
- Humidity: 60% (+/- 5%)
- Air change: 15 times per hour
- Illumination: 12 hour light/dark rhythm

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

- undiluted application
- Single dose after 16 h of fasting
- Concentration: 10 ml/kg

Doses:

10 000 mg/kg bw (gavage),

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Post dose observation period: 14 days
EXAMINATIONS:
- body weight: before and on days 1, 7, 14 after treatment
- clinical signs: up to 6 hours after treatment, then daily
- gross pathology at the end of investigation

Statistics:

not neccessary

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 10 000 mg/kg bw

Mortality:

no mortality

Clinical signs:

other: About 45 minutes after adminstration the animals showed ruffeld fur, staggering, prone position, Straub's phenomenon, slight sedation and ataxia. Signs of toxicity had disappeared after 24 hours.

Gross pathology:

no findings

Other findings:

no further information

Any other information on results incl. tables

no further remarks

Applicant's summary and conclusion

Conclusions:

In a determination of the acute oral toxicity on male and female rats it was found that the LD50 of the test item isopropylcyclohexane is greater than 10000 mg/kg body weight. Under the conditions of this study Isopropylcyclohexane is practically nontoxic after oral application in rats.

Executive summary:

The test item was given to rats by oral administration to obtain information on the toxicity, in particular lethality, of the test item.

Isopropylcyclohexane was administrated oral to 5 male and 5 female WISTAR rats. There was no mortality and no influence on the increase in body weight. About 45 minutes after adminstration the animals showed ruffeld fur, staggering, prone position, Straub's phenomenon, slight sedation and ataxia. Signs of toxicity has disappeared after 24 hours. Dissection at the end of the experiment showed no findings.

Under the conditions of this study the acute toxicity after oral application is greater than 10000 mg/kg bw. Therefore, isopropylcyclohexane is practically nontoxic after oral application in rats.