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EC number: 246-042-5 | CAS number: 24155-42-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-09-10 to 2008-03-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 00512242
- Expiration date of the lot/batch: 2013-06-27 (retest date)
- Purity test date: 2007-08-21 (certificate of analysis release date)
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: cool, dry, tighly closed
- Stability under test conditions: stability in test solutions was verified by analytical determinations - Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples were taken at 0h, from fresh test solution and after 24,48 and 72h from aged and fresh test solution and after 96 hours from aged test solution.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substance):
A stock solution containing 1000 µg/mL of the test item was prepared by weighting 20.2 mg of the test item into a 20 mL volumetric flask and adjusting the volume to 20.2 mL with acetonitrile. From the stock solutions, dilutions containing 10 µg/mL and 1 µg/mL were prepared in acetonitrile. From the 1 µg/mL dilution, a dilution containing 0.1 µg/mL was prepared in acetonitrile/water 2:10 (v/v) and used for preparation of calibration curve. All solutions were stored refrigerated. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Fischzucht Wagenhause, Germany
- Length at study initiation (length definition, mean, range and SD): 4-6 cm
- Feeding during test: feeding was stopped 24 h before initiation of test
ACCLIMATION
- Acclimation period: >12 days
- Acclimation conditions (same as test or not): testing conditions in 300 L containers with continuous renewal of water (5-10% per day) and permanent aeration of water.
- the fish were fed each day with granular rearing food with approximately 2% of their bodyweight. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 14°dH
- Test temperature:
- 15.4-16.8°C
- pH:
- 7.88-8.5
- Dissolved oxygen:
- >91% of air saturation (approximately 9.1 mg O2/L)
- Conductivity:
- 467 µS/cm
- Nominal and measured concentrations:
- Range-finder:
Nominal concentrations (mg/L): 0, 0.01, 0.1, 1, 10, 100
Final test
nominal concentrations (mg/L): 0, (1.563), (3.125), 6.25, 12.5, 25, 50, (100) - () not analysed
t=0h (mg/L): < LOQ, 3.20, 8.88, -, 34.80
t=24h aged:t=24h fresh: t=48h aged: t=48h fresh: t=72h aged: t=72h fresh: t=96h aged: - Details on test conditions:
- TEST SYSTEM
- Test vessel: 25L stainless steel tanks, filled with 24L
- No. of organisms per vessel: 10 per concentration
- No. of vessels per concentration (replicates): no replicates
- Biomass loading rate: max loading of 1g fish/L
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortaility, clinical signs was checked at 3h,6h,24h,48h,72h,96h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: x2
- Range finding study: yes
- Test concentrations:0-0.01-1-10-100 mg/L
- Results used to determine the conditions for the definitive study: In the nominal concentrations up to 10 mg/L no fish died, but at the nominal concentration of 100 mg/L all fish died within a period of 24 hours. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 28.06 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: C.L.: 19.087-41.251 mg/L
- Details on results:
- - Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: unusual behaviour (reduced activity, change in pigmentation and or orientation to bottom or surface of the test vessels), difficulties with maintenance of equilibrium
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- LC0 (96h) was observed at 6.25 mg/L
- LC100 (96h) was observed at 50 mg/L.
- LC50 (24h)= 84.08 mg/L (CL: 56.114-125.976)
- LC50 (48h)=34.02 mg/L (CL: 30.630-37.779)
- LC50 (72h) = 32.42 mg/L (CL: 19.087-41.251)
- NOEC = 6.25 mg/L - Reported statistics and error estimates:
- Due to the distribution of the data, the LC50 was calculated using the Moving Average Computations.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of T000824 to Rainbow trout was determined in a 96-hour semi-static test according to OECD guideline 203. Under the conditions of the present study, the 96-h LC50 was determined to be 28.06 mg/L, whereas the NOEC was 6.25 mg/L (nominal concentrations). The results of the test can be considered reliable without restriction.
Reference
Description of key information
The study of Gonsior (2008), investigating the acute toxicity of T000824 in rainbow trout according to OECD guideline 203, was considered as the key study for endpoint coverage. An LC50 of 28.06 mg/L (based on nominal test concentrations) was obtained after an exposure of 96 hours in a semi-static test in freshwater. Based on the results it can be considered that T000824 is harmful to fish.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 28.06 mg/L
Additional information
One key study (Klimisch sore of 1) was available on the acute toxicity of T000824 to Rainbow trout. The key study exposed Rainbow trout for 96 hours to T000824 under semi-static conditions. The nominal test item concentrations were
1.563, 3.125, 6.25, 12.5, 25, 50, 100 mg/L and in parallel the control. Based on the nominal test concentrations a 96 -h LC50 of 28.06 mg/L for T000824.
Based on the results it can be considered that T000824 is harmful to fish.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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