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EC number: 617-849-3 | CAS number: 86404-04-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 - 29 Jan 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Health Care Inspectorate of the Ministry of Health, Welfare and Sport, Utrecht, The Netherlands
Test material
- Reference substance name:
- (5R)-5-[(1S)-1,2-dihydroxyethyl]-4-ethoxy-3-hydroxy-2,5-dihydrofuran-2-one
- EC Number:
- 617-849-3
- Cas Number:
- 86404-04-8
- Molecular formula:
- C8H12O6
- IUPAC Name:
- (5R)-5-[(1S)-1,2-dihydroxyethyl]-4-ethoxy-3-hydroxy-2,5-dihydrofuran-2-one
- Details on test material:
- - Name of test material (as cited in study report): Ethyl ascorbic acid
- Physical state: powder
- Expiration date of the lot/batch: 21 Apr 2018
- Stability under test conditions: stable
- Storage condition of test material: in freezer at < -15 °C desiccated
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 0.1, 1.0, 10, 100 mg/L
- Sampling method: Samples were taken after 0 and 48 h. 2 mL were taken from the approximate centre of the test vessels.
- Sample storage conditions before analysis: Samples taken at the beginning of the study were stored in a freezer until analysis whereas samples taken at the end of the study were analysed immediately. At the end of exposure, the replicates were pooled at each concentration before sampling. Additionally, reserve samples of 2.0 mL were taken for possible analysis. If not used, these samples were stored in a freezer for a maximum of three months.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The highest test concentration of 100 mg/L was prepared by adding appropriate amount of the test substance to test medium followed by vigorous mixing. The lower concentrations were prepared by subsequent dilution of this stock solution.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: in-house culture with a known history
- Age at study initiation (mean and range, SD): < 24 h (from parental daphnids of more than two weeks old)
- Method of breeding: With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium in an all-glass culture vessel. The culture was 4 weeks old (maximum). Cultures were renewed after 7 d of cultivation (half of the medium). Daphnids were fed with a suspension of freshwater algae. The medium was M7 medium
- Feeding during test: none
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 19 - 20 °C
- pH:
- 8.1 - 8.2 (control)
7.2 - 7.8 (highest test concentration) - Dissolved oxygen:
- 8.9 - 9.7 mg O2/L
- Nominal and measured concentrations:
- nominal: control, 0.1, 1.0, 10, 100 mg/L
measured: < LOQ, 0.068, 0.80, 7.8, 78 mg/L (average) - Details on test conditions:
- TEST SYSTEM
Test vessel
- Material, size, headspace, fill volume: glass, 100 mL, headspace: 20 mL, fill volume: 80 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2 (for 0.10, 1.0 and 10 mg/L), 4 (for 100 mg/L)
- No. of vessels per control (replicates): 5
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: same as test
- Intervals of water quality measurement: The pH and dissolved oxygen at control and 100 mg/L were measured at the beginning and end of exposure whereas temperature was measured continuously.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
EFFECT PARAMETERS MEASURED
- Mobility: after 24 and 48 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (tested in a separate study in December 2015)
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 78 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0%
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50/LC50: EC50 (48 h): 0.38 mg/L (95% CI: 0.33 - 0.43 mg/L) - Reported statistics and error estimates:
- No statistical analysis of the data were performed since no effects were observed in order to derive an EC50 value.
Any other information on results incl. tables
Table 1: Measured concentrations
Nominal concentration [mg/L] |
Measured at t=0 h [mg/L] |
Measured at t=48 h [mg/L] |
Average concentration (geometric mean) [mg/L] |
0.10 |
0.077 |
0.060 |
0.068 |
1.0 |
0.87 |
0.78 |
0.80 |
10 |
7.5 |
8.1 |
7.8 |
100 |
74 |
82 |
78 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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