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EC number: 207-803-7 | CAS number: 495-54-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- growth inhibition effect on activated sludge microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- The growth inhibition effect of the test substance 4-(phenylazo)benzene-1,3-diamine on activated sludge microorganisms was studied.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Remarks:
- 2 % aqueous ethanol solution
- Details on test solutions:
- Test concentration: 0.17 m.mole/L (maximum experimental concentration)
- Test organisms (species):
- activated sludge
- Details on inoculum:
- The mixed microorganisms were obtained from supernatant of activated sludge and pre-incubated with the medium without azo compounds for 2 days. The microorganisms were inoculated to the experimental medium to give the optical density of the culture at 0.02-0.05. Incubation temperature was at 25±1 deg. C
- Test type:
- static
- Water media type:
- freshwater
- Remarks on exposure duration:
- Not specified
- Post exposure observation period:
- Not specified
- Hardness:
- Not specified
- Test temperature:
- 25±1 deg. C
- pH:
- Not specified
- Dissolved oxygen:
- Not specified
- Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Test concentration: 0.17 m.mole/L (maximum experimental concentration)
- Details on test conditions:
- Culture Medium is as follows:
Glucose: 500 ppm
Peptone: 500 ppm
KH2PO4:0 270 ppm
K2HPO4: 100 ppm
Na2HPO4: 660 ppm
MgSO4. 7H20: 2 ppm
CaCI2: 0.0200 ppm
FeSO4. 7H20: 0.0200 ppm
in 2 % aqueous ethanol solution - Reference substance (positive control):
- not specified
- Key result
- Dose descriptor:
- other: IGC50
- Effect conc.:
- 0.087 mmol/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: duration not specified
- Details on results:
- Not specified
- Results with reference substance (positive control):
- Not specified
- Reported statistics and error estimates:
- Not specified
- Validity criteria fulfilled:
- not specified
- Conclusions:
- 50 % growth inhibition concentration (IGC50) of the test substance 4-(phenylazo)benzene-1,3-diamine on mixed culture of microorganisms was observed to be 0.087 m.mole/L. The study concludes that the test substance showed strong growth inhibition effect on the microorganisms.
- Executive summary:
In order to evaluate the effect of test substance 4-(phenylazo)benzene-1,3-diamine on the biological waste treatment, the growth inhibition effect of it on activated sludge microorganisms was studied. Growth inhibition effect was evaluated by the concentration of test compound in which logarithmic phase growth rate constant was decreased to 50 % of that of the culture without test compound. Growth rate constant was determined with turbidity of that of the culture at 660 nm. The mixed microorganisms were obtained from supernatant of activated sludge and pre-incubated with the medium without azo compounds for 2 days. The microorganisms were inoculated to the experimental medium to give the optical density of the culture at 0.02-0.05. Incubation temperature was at 25±1 deg C. 50 % growth inhibition concentration (IGC50) of the test substance 4-(phenylazo)benzene-1,3-diamine on mixed culture of microorganisms was observed to be 0.087 m.mole/L. The study concludes that the test substance showed strong growth inhibition effect on the microorganisms.
Reference
50 % Growth Inhibition Concentrations (Ø) of test substance:
Compound |
Medium |
log Ø |
log C |
log Poct |
2,4-DIAMINOAZOBENZENE [4-(phenylazo)benzene-1,3-diamine] |
e |
-1.056 |
-0.752 |
2.19 |
Ø;
concentration of 50 % growth inhibition (m.mole/l.)
C; maximum experimental concentration (m.mole/l.)
Poct; partition coefficient between octanol and water
e; 2 % aqueous ethanol solution
Description of key information
50 % growth inhibition concentration (IGC50) of the test substance 4-(phenylazo)benzene-1,3-diamine on mixed culture of microorganisms was observed to be 0.087 m.mole/L. The study concludes that the test substance showed strong growth inhibition effect on the microorganisms.
Key value for chemical safety assessment
Additional information
In order to evaluate the effect of test substance 4-(phenylazo)benzene-1,3-diamine on the biological waste treatment, the growth inhibition effect of it on activated sludge microorganisms was studied. Growth inhibition effect was evaluated by the concentration of test compound in which logarithmic phase growth rate constant was decreased to 50 % of that of the culture without test compound. Growth rate constant was determined with turbidity of that of the culture at 660 nm. The mixed microorganisms were obtained from supernatant of activated sludge and pre-incubated with the medium without azo compounds for 2 days. The microorganisms were inoculated to the experimental medium to give the optical density of the culture at 0.02-0.05. Incubation temperature was at 25±1 deg C. 50 % growth inhibition concentration (IGC50) of the test substance 4-(phenylazo)benzene-1,3-diamine on mixed culture of microorganisms was observed to be 0.087 m.mole/L. The study concludes that the test substance showed strong growth inhibition effect on the microorganisms.
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