3-nitrobenzoic acid

Endpoint summary

Description of key information

Bioaccumulation: aquatic / sediment

Bioaccumulation study was conducted on test organism Cyprinus carpiofor 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical (authoritative database, 2018). The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances" under flow through conditions at a temperature of 25°C and pH range 6.0-8.5, respectively. Cyprinus carpio (length - 8 dimensionless) was used as a test organism for the study. Test chemical nominal conc. used for the study was 0.3mg/l and 0.03 mg/l, respectively. Analytical method involve therecovery ratio:Test water : 1st concentration area : 94.3 %, 2nd concentration area : 98.6 %, Fish : 88.5 %, - Limit of detection : Fish : 0.188 ppm. Range finding study involve theTLm(48h) ≥ 50 ppm (w/v) on Rice fish (Oryzias latipes). Lipid content of the test organism was determined to be 2.8%.The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be≤ 0.7 L/Kg at a conc. of 0.3 mg/l and ≤ 7.1 L/Kg at a conc. of 0.03 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.

Additional information

Bioaccumulation: aquatic / sediment

Various experimental key and supporting studies of the test chemical were reviewed for the bioaccumulation end point which are summarized as below:

 

In an experimental key study from authoritative database (2018), bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical. The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances" under flow through conditions at a temperature of 25°C and pH range 6.0-8.5, respectively. Cyprinus carpio (length - 8 dimensionless) was used as a test organism for the study. Test chemical nominal conc. used for the study was 0.3mg/l and 0.03 mg/l, respectively. Analytical method involve therecovery ratio: Test water : 1st concentration area : 94.3 %, 2nd concentration area : 98.6 %, Fish : 88.5 %, - Limit of detection : Fish : 0.188 ppm. Range finding study involve the TLm(48h) ≥ 50 ppm (w/v) on Rice fish (Oryzias latipes). Lipid content of the test organism was determined to be 2.8%.The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be ≤ 0.7 L/Kg at a conc. of 0.3 mg/l and ≤ 7.1 L/Kg at a conc. of 0.03 mg/l, respectively.

 

Another bioaccumulation study was conducted for estimating the BCF (bioaccumulation factor) value of test chemical (HSDB and PubChem, 2017). The bioaccumulation factor (BCF) value was calculated using a logKow of 1.83 and a regression-derived equation. The estimated BCF (bioaccumulation factor) value of test chemical was determined to be 3.2 dimensionless.

 

In aprediction done using theKOCWIN Programof Estimation Programs Interface was used to predict the bioconcentration factor (BCF) of test chemical. The bioconcentration factor (BCF) of test chemical was estimated to be 3.162 L/kg whole body w.w (at 25 deg C).

 

Using Bio-concentration Factor (v12.1.0.50374) module Bio-concentration Factor of the test chemical was estimated to be 14.3, 13.8, 10.7 and 3.33 dimensionless at range pH range 0-1, 2, 3, 4  and 5-14, respectively (ACD (Advanced Chemistry Development)/I-Lab predictive module, 2017)).

 

On the basis of above results for test chemical(from authoritative and modelling databases, 2017), it can be concluded that the BCF value of test chemicalranges from0.7to14.3,which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.