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EC number: 205-047-2 | CAS number: 132-16-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Short term toxicity to aquatic invertebrate:
Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 1 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. DMSO used as a solvent, because of limited solubility of tested sample. Chemical was tested at 0, 0, 1.2, 2.4, 5, 10 and 20 mg/l nominal concentrations. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 4.43 mg/L on the basis of mobility inhibition effects in a 48 hour study.
Toxicity to aquatic algae and cyanobacteria:
Aim of this study was to determine the freshwater algal growth inhibition test on the growth of Desmodesmus subspicatus with the test substance. Test was conducted according to OECD Guideline 201. The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation. Test conducted at limit test concentration 21 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration after the exposure of 72hrs.
Additional information
Short term toxicity to aquatic invertebrate:
Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 1 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. DMSO used as a solvent, because of limited solubility of tested sample. Chemical was tested at 0, 0, 1.2, 2.4, 5, 10 and 20 mg/l nominal concentrations. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 4.43 mg/L on the basis of mobility inhibition effects in a 48 hour study.
Toxicity to aquatic algae and cyanobacteria:
Aim of this study was to determine the freshwater algal growth inhibition test on the growth of Desmodesmus subspicatus with the test substance. Test was conducted according to OECD Guideline 201. The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation. Test conducted at limit test concentration 21 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration after the exposure of 72hrs.
On the basis of all above effects and results, it was observed that the chemical was insoluble in water and thus the study performed by dissolving chemical in vehicle and limit test were performed at higher solubility level. Thus by conseding the criteria of insolubility chemical cannot be consider to be toxic and not classified as per the CLP classification criteria.
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