Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)-3,4,5,6-tetrachlorobenzoate]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Justification for type of information:

Experimental test result performed using standard OECD test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the zebrafish (Danio rerio).The nominal exposure concentration was 0.778 mg/L i.e. the solubility limit of the tested chemical.

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material(as cited in study report):Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)-3,4,5,6-tetrachlorobenzoate]
- Molecular formula:C20H4Br4Cl4O5 2/3Al
- Physical state:Solid
-Nature of chemical: Organic

Analytical monitoring:

no

Details on sampling:

No data available

Vehicle:

not specified

Details on test solutions:

No data available

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Source:Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD):3.42 cm
- Weight at study initiation (mean and range, SD):0.335 g
- Feeding during test:yes
- Food type:Standard brand feed
- Frequency:once daily

ACCLIMATION
- Acclimation period:7 days
- Acclimation conditions (same as test or not):same as test
- Type and amount of food:Standard brand feed
- Feeding frequency:Once in a day up to sixth day of acclimatization
- Health during acclimation (any mortality observed):No mortality
-Aeration:Continuous aeration was provided
HOUSING
-Duration of housing:15 days
-Photoperiod:12 hours light and 12 hours darkness
-Oxygen content:7.06 (mg/l)
-pH:7.23
-Water temperature:24 °C

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

No data available

Post exposure observation period:

24, 48, 72 and 96 hours.

Hardness:

147 mg of CaCO3

Test temperature:

25°C

pH:

7.17

Dissolved oxygen:

No data

Salinity:

No data

Conductivity:

No data available

Nominal and measured concentrations:

Nominal Concentration : Limit Test = 0.778 mg/L

Details on test conditions:

-Test vessels:The test vessels used were all plastic aquaria having the water capacity of 5liters.-Photoperiod:12 hours light and 12-hour darkness-Volume of water:2 liters-No. of animals:8 fishes

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 0.778 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

he LC0 value was considered to be above the solubility limit for the tested chemical.

Basis for effect:

mortality (fish)

Remarks on result:

other: No mortalities were found in the control aquaria.

Details on results:

Determination or calculation of the median lethal concentrations (LC0) and observations of the symptoms were done after 24, 48, 72 and 96 hours.

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

No data available

Validity criteria fulfilled:

not specified

Conclusions:

The LC0 value was considered to be above the solubility limit for the tested chemical.

Executive summary:

A fish acute toxicity test according to OECD Guideline 203 was conducted for dialuminiumtris [2-(2, 4, 5, 7-tetrabromo-6 -oxido- 3-oxo xanthen-9-yl)-3, 4, 5, 6-tetrachlorobenzoate]. The nominal exposure concentration was 0.778 mg/L i.e. the solubility limit of the tested chemical. The test organism was zebrafish (Danio rerio). One had two tanks per concentration and 4 fish per tank (8 fish in total per concentration). The water control contained the same number of fish. One observed no mortalities or abnormal behaviour in these fish tanks. The exposure time was 96 hours. The exposed fish were swimming a bit slower after 96 hours. However, no mortalities were observed in the exposure tanks. The LC0 was therefore predicted to be >0.778 mg/L. The LC0 value was considered to be above the solubility limit for the tested chemical.

Description of key information

A fish acute toxicity test according to OECD Guideline 203 was conducted for dialuminiumtris [2-(2, 4, 5, 7-tetrabromo-6 -oxido- 3-oxo xanthen-9-yl)-3, 4, 5, 6-tetrachlorobenzoate]. The nominal exposure concentration was 0.778 mg/L i.e. the solubility limit of the tested chemical. The test organism was zebrafish (Danio rerio). One had two tanks per concentration and 4 fish per tank (8 fish in total per concentration). The water control contained the same number of fish. One observed no mortalities or abnormal behaviour in these fish tanks. The exposure time was 96 hours. The exposed fish were swimming a bit slower after 96 hours. However, no mortalities were observed in the exposure tanks. The LC0 was therefore predicted to be >0.778 mg/L. The LC0 value was considered to be above the solubility limit for the tested chemical.

Additional information

A fish acute toxicity test according to OECD Guideline 203 was conducted for dialuminiumtris [2-(2, 4, 5, 7-tetrabromo-6 -oxido- 3-oxo xanthen-9-yl)-3, 4, 5, 6-tetrachlorobenzoate]. The nominal exposure concentration was 0.778 mg/L i.e. the solubility limit of the tested chemical. The test organism was zebrafish (Danio rerio). One had two tanks per concentration and 4 fish per tank (8 fish in total per concentration). The water control contained the same number of fish. One observed no mortalities or abnormal behaviour in these fish tanks. The exposure time was 96 hours. The exposed fish were swimming a bit slower after 96 hours. However, no mortalities were observed in the exposure tanks. The LC0 was therefore predicted to be >0.778 mg/L. The LC0 value was considered to be above the solubility limit for the tested chemical.