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EC number: 241-922-5 | CAS number: 18015-76-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- December, 1982 - February, 1983
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The global experiment is well documented and scientifically acceptable; nevertheless details about the tested substance composition are missing. Read across from a similar substance which has the same main component and with a different counter ion that does not influence the characteristics related to the specific end-point.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
- Principles of method if other than guideline:
- The test item acute oral toxicity was tested on male and female wistar rats. The substance was administered by oral gavage in dosage of 0.5, 0.8, 1.3, 1.6, 2.0 ml/kg/bw. The duration of observation period following administration was of 14 days, then animals were necropsied.
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Methanaminium, N-[4-[[4-(dimethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-, ethanedioate
- EC Number:
- 241-922-5
- EC Name:
- Methanaminium, N-[4-[[4-(dimethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-, ethanedioate
- Cas Number:
- 18015-76-4
- Molecular formula:
- C23H25N2 x C2HO4
- IUPAC Name:
- 4-{[4-(dimethylamino)phenyl](phenyl)methylidene}-N,N-dimethylcyclohexa-2,5-dien-1-iminium hydrogen oxalate
- Test material form:
- other: liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen.
- Age at study initiation: 98 days old.
- Number of animals: 9 males, 14 females.
- Weight at study initiation: males 165 -184 g and females 160-182 g.
- Diet: pellet Alrtomin R 1324, ad libitum.
- Water: tap water, ad libitum.
- Other: all animals were marked into skin by picric acid.
ENVIRONMENTAL CONDITIONS
- Temperature: 22 ± 1.5 °C
- Humidity: 60 ± 5 %
- Photoperiod: 12 hrs dark/light cycle, artificial light.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Doses:
- 0.5, 0.8, 1.3, 1.6, 2.0 ml/kg/bw
- No. of animals per sex per dose:
- 5 animal x each sex x dose
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: twice a day.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs. - Statistics:
- The calculation of the LD-50 for the confidence interval p <0.05 was conducted in accordance with to Rosier et al.J. Tox. Environ. Health 3, 1977, 797th.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1.2 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- 1.1 - 1.4
- Mortality:
- All animals are dead by a dose of 2 ml/kg bw; the deaths were within the first 6 days after the application registered.
- Clinical signs:
- other: Dose levels between 0.8 to 2.0 ml/kg: in all the animals worsening health and bristling. At doses ≥ 1.3 ml/kg:: manifest signs of relaxation and abdominal numbness. At doses ≥ 0.8 ml / kg: growth retardation. The symptoms occurred about 1 to 2 hours afte
Any other information on results incl. tables
Group | Dose [ml/kg bw] |
Result | Symptoms duration | Dead time | Mortality |
Males and females | |||||
1 | 0.5 | 0/0/10 | -- | -- | 0 |
2 | 0.8 | 2/10/10 | 24h - 14d | 3d - 6d | 20 |
3 | 1.3 | 5/10/10 | 2h - 7d | 4h - 5d | 50 |
4 | 1.6 | 7/10/10 | 1h - 14d | 2h - 3d | 70 |
5 | 2.0 | 10/10/10 | 1h - 2d | 2h - 3d | 100 |
Applicant's summary and conclusion
- Interpretation of results:
- other: Acute tox 4 (H302), according to the CLP Regulation (EC 1272/2008)
- Remarks:
- Criteria used for interpretation of results: EU
- Conclusions:
- LD50 (rat): 1200 mg/kg/bw
- Executive summary:
The test item acute oral toxicity was tested on male and female wistar rats. The substance was administered by oral gavage in dosage of 0.5, 0.8, 1.3, 1.6, 2.0 ml/kg/bw. The duration of observation period following administration was of 14 days, then animals were necropsied.
All animals are dead by a dose of 2 ml/kg bw; the deaths were within the first 6 days after the application registered. Dose levels between 0.8 to 2.0 ml/kg caused in all the animals worsening health and bristling. At 1.3 ml/kg manifest signs of relaxation and abdominal numbness were recorded. At 0.8 ml/kg growth retardation was observed. The symptoms occurred about 1 to 2 hours after application and kept in individual animals until the end of the experiment. The LD50 was identified to be 1.2 ml/kg bw.
Conclusion
LD50 (rat): 1200 mg/kg/bw
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