Sodium [3-hydroxy-4-[(1-hydroxy-5-sulpho-2-naphthyl)azo]naphthalene-1-sulphonato(4-)]chromate(1-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

No data

Vehicle:

not specified

Details on test solutions:

M4 medium

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: RCC Ltd.
- Age at study initiation (mean and range, SD): Young daphnids, aged less than ‘24’ hours old

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

220 mg/L

Test temperature:

20.7- 20.8 °C

pH:

7.69 - 7.88

Dissolved oxygen:

7.63 – 7.78 mg/L

Salinity:

Not available

Conductivity:

Not available

Nominal and measured concentrations:

0.30, 0.60, 1.20 2.40, 4.80, 9.60 and 19.20 mg/L

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 10 daphnids

Reference substance (positive control):

yes

Remarks:

Potassium Dichromate Pure

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

4.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

1.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

Based on the test results, the EC50 of Potassium Dichromate Pure in Daphnia magna observed for a period of ‘24’ hour was calculated as 1.72 mg/L with upper confidence limits of 2.12 mg/L and lower confident limits of 1.40 mg/L and regression equation Y = 4.0957 + 3.8604 log (x). At ‘48’ hour, the EC50 was calculated as 1.33 mg/L with upper confidence limits of 1.63 mg/L and lower confidence limits of 1.08 mg/L and regression equation Y = 4.5239 + 3.8769 log (x).

Reported statistics and error estimates:

None

No immobilization was observed in control and daphnids exposed to 0.30 and 0.60 mg/L test concentration throughout the experimental period. At the end of 24 hour, no immobility was observed in daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 7/40 and 14/40 immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. Thus, the percent immobilisation at the end of 24 hour was recorded to be 0 % in control and daphnids exposed to 0.30, 0.60, 1.20, 2.40 and 4.80 mg/L concentrations whereas, 17.5 % and 35 % was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. At the end of 48 hour, 3/40, 7/40 and 14/40 immobility was recorded in the daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 39/40, 40/40 cumulative immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentration. Thus the percent cumulative immobilisation at the end of 48 hour was recorded to be 0% in control and daphnids exposed to 0.30, and 0.60 mg/L test concentrations whereas, 7.5 %, 17.5 %, 35 %, and 97.5 % was observed in daphnids exposed to 1.20, 2.40, 4.80 and 9.60 mg/L concentrations and 100 % was observed in daphnids exposed to 19.20 mg/L concentration. 

Based on the test results, the EC50 of  FAT 20290/C TE  in Daphnia magna observed for a period of 24 hour was calculated as 24.07 mg/L with upper confidence limits of 43.27 mg/L and lower confidence limits of 18.27 mg/L and regression equation Y = 0.8956 +2.9711 log (x).

The EC50 of FAT 20290/C TE  in Daphnia magna observed for a period of 48 hour was calculated as 4.40 mg/L with upper confidence limits of 6.64  mg/L and lower confidence limits of 2.93 mg/L and regression equation Y = 2.7293+3.5279 log (x).

The no observed effect concentration (NOEC) and the low observed effect concentration (LOEC) for 24 hours was calculated as 4.80 mg/L and 9.60 mg/L respectively.

The no observed effect concentration (NOEC) and the low observed effect concentration (LOEC) for 48 hours was calculated as 0.60 mg/L  and 1.20 mg/L respectively.

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of FAT 20290/C TE in Daphnia magna observed for a period of 48 hour was calculated as 4.40 mg/L.

Executive summary:

Acute Immobilization Toxicity Study in Daphnia magna was conducted in accordance with OECD guideline 202 with FAT 20290/C TE. Based on the results of the range finding experiment, main experiment was conducted with a range of concentrations viz., 0.30, 0.60, 1.20, 2.40, 4.80, 9.60 and 19.20 mg/L.

No immobilization was observed in control and daphnids exposed to 0.30 and 0.60 mg/L test concentration throughout the experimental period. At the end of 24-hour observation period, no immobility was observed in daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 7/40 and 14/40 immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. Thus, the percent immobilization at the end of 24-hour observation period was recorded to be 0 % in control and daphnids exposed to 0.30, 0.60, 1.20, 2.40 and 4.80 mg/L concentrations whereas, 17.5 % and 35 % was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. At the end of 48-hour observation period, 3/40, 7/40 and 14/40 immobile animals were recorded in the daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 39/40, 40/40 cumulative immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentration. Thus, the percent cumulative immobilization at the end of 48 hour was recorded to be 0 % in control and daphnids exposed to 0.30, and 0.60 mg/L test concentrations whereas, 7.5 %, 17.5 %, 35 %, and 97.5 % was observed in daphnids exposed to 1.20, 2.40, 4.80 and 9.60 mg/L concentrations and 100 % was observed in daphnids exposed to 19.20 mg/L concentration. 

The EC50 of FAT 20290/C TE in Daphnia magna observed for a period of 48 hour was calculated as 4.40 mg/L with upper confidence limits of 6.64 mg/L and lower confidence limits of 2.93 mg/L and regression equation Y = 2.7293+3.5279 log (x).

The no observed effect concentration (NOEC) and the low observed effect concentration (LOEC) for 48 hours was calculated as 0.60 mg/L and 1.20 mg/L, respectively.

Description of key information

The EC50 of FAT 20290/C TE in Daphnia magna observed for a period of 48 hours was calculated as 4.40 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

4.4 mg/L

Additional information

Acute Immobilization Toxicity Study in Daphnia magna was conducted in accordance with OECD guideline 202 with FAT 20290/C TE. Based on the results of the range finding experiment, main experiment was conducted with a range of concentrations viz., 0.30, 0.60, 1.20, 2.40, 4.80, 9.60 and 19.20 mg/L.

No immobilization was observed in control and daphnids exposed to 0.30 and 0.60 mg/L test concentration throughout the experimental period. At the end of 24-hour observation period, no immobility was observed in daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 7/40 and 14/40 immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. Thus, the percent immobilization at the end of 24-hour observation period was recorded to be 0 % in control and daphnids exposed to 0.30, 0.60, 1.20, 2.40 and 4.80 mg/L concentrations whereas, 17.5 % and 35 % was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. At the end of 48-hour observation period, 3/40, 7/40 and 14/40 immobile animals were recorded in the daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 39/40, 40/40 cumulative immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentration. Thus, the percent cumulative immobilization at the end of 48 hour was recorded to be 0 % in control and daphnids exposed to 0.30, and 0.60 mg/L test concentrations whereas, 7.5 %, 17.5 %, 35 %, and 97.5 % was observed in daphnids exposed to 1.20, 2.40, 4.80 and 9.60 mg/L concentrations and 100 % was observed in daphnids exposed to 19.20 mg/L concentration. 

The EC50 of FAT 20290/C TE in Daphnia magna observed for a period of 48 hour was calculated as 4.40 mg/L with upper confidence limits of 6.64 mg/L and lower confidence limits of 2.93 mg/L and regression equation Y = 2.7293+3.5279 log (x).

The no observed effect concentration (NOEC) and the low observed effect concentration (LOEC) for 48 hours was calculated as 0.60 mg/L and 1.20 mg/L, respectively.