Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 223-095-2 | CAS number: 3734-33-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental study using one of the recommended OECD guidelines.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Principles of method if other than guideline:
- Biodegradation study was conducted according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test) for evaluating the percentage biodegradability of test substance N-benzyl-2-[(2,6-dimethylphenyl)amino]-N,N-diethyl-2-oxoethanaminium benzoate hydrate (CAS no. 3734-33-6).
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): N-benzyl-2-[(2,6-dimethylphenyl)amino]-N,N-diethyl-2-oxoethanaminium benzoate hydrate
- Molecular formula: C21H29N2O.C7H5O2
- Molecular weight: 446.58 g/mol
- Smilies notation: CC[N+](CC)(Cc1ccccc1)CC(=O)Nc2c(cccc2C)C.c1ccc(cc1)C(=O)[O-]
- InChl: 1S/C21H28N2O.C7H6O2/c1-5-23(6-2,15-19-13-8-7-9-14-19)16-20(24)22-21-17(3)11-10-12-18(21)4;8-7(9)6-4-2-1-3-5-6/h7-14H,5-6,15-16H2,1-4H3;1-5H,(H,8,9)
- Substance type: organic
- Physical state: White granular solid - Oxygen conditions:
- aerobic
- Inoculum or test system:
- mixture of sewage, soil and natural water
- Details on inoculum:
- Mixed Inoculum Preparation (In-House): Domestic waste water, surface soil and soil samples from polluted sites receiving predominantly domestic waste was collected and were mixed to get diluted suspension. The inoculum was kept aerobic until being used for experiment by supplying organic and inorganic sources required by micro flora to sustain at controlled laboratory conditions. This gave the bacterial count as107to 108CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- other: BOD and ThOD
- Key result
- Parameter:
- other: BOD
- Value:
- 18.17
- Sampling time:
- 28 d
- Remarks on result:
- other: Other details not known
- Details on results:
- The oxygen consumed by the test systems was corrected for oxygen consumption occurring in the blank test systems. The BOD Values (mgO2/mg) and percent biodegradation results for each test system are reported in Tables 2 and 3, respectively.
The BOD28 value of Denatonium benzoate (CAS No. 3734-33-6) was observed to be 0.436 mgO2/mg. ThoD was determined by calculation as 2.40 mgO2/mg. % Degradation was calculated using the values of BOD and ThOD for test item and was found to be 18.17%. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was found to be 51.39%.
The mean BOD values (mg O2/l) in control on 28th day was 28.2 mg O2/l, thereby fulfilling the control validity criteria (i.e., the oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days).
Degradation of Sodium benzoate exceeds 44.16% after 7 days and 50.24% after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid. - Results with reference substance:
- Degradation of Sodium benzoate exceeds 44.16% after 7 days and 50.24% after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test item Denatonium benzoate (CAS No. 3734-33-6) undergoes 18.17% biodegradation after 28 days in the test condition. Thus, the test item can be inferred as non-biodegradable.
- Executive summary:
28-days Manometric respirometry test following the OECD guideline 301F to determine the ready biodegradability of the test item Denatonium benzoate (CAS No. 3734-33-6) was conducted. The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 100mg/L, while that of inoculum was 10ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28value of Denatonium benzoate (CAS No. 3734-33-6) was observed to be 0.436 mgO2/mg. ThOD was calculated as 2.40mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was determined to be 18.17%. Based on the results, the test item, under the test conditions, was considered to be non-biodegradable at 20 ± 1°C over a period of 28 days.
Reference
BOD28, THOD AND % BIODEGRADATION VALUES
Method details |
BOD28(mgO2/mg) |
ThOD (mgO2/mg) |
% Biodegradation |
Test Item |
0.436 mgO2/mg |
2.40 mgO2/mg |
18.17 % |
Reference Item |
0.853 mgO2/mg |
1.66 mgO2/mg |
51.39 % |
Description of key information
28-days Manometric respirometry test following the OECD guideline 301F to determine the ready biodegradability of the test item Denatonium benzoate (CAS No. 3734-33-6) was conducted. The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 100mg/L, while that of inoculum was 10ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28value of Denatonium benzoate(CAS No. 3734-33-6) was observed to be 0.436 mgO2/mg. ThOD was calculatedas 2.40mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was found to be 18.17%. Based on the results, the test item, under the test conditions, was found to be non-biodegradable at 20 ± 1°C over a period of 28 days.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Experimental study was done by UERL laboratory (2015) in this study a 28-days Manometric respirometry test following the OECD guideline 301F was done to determine the ready biodegradability of the test item Denatonium benzoate (CAS No. 3734-33-6) . The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 100mg/L, while that of inoculum was 10ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28value ofDenatonium benzoate (CAS No. 3734-33-6) was observed to be 0.436 mgO2/mg. ThOD was calculatedas 2.40mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was obtained to be 18.17%. Based on the results, the test item, under the test conditions, was considered to be non-biodegradable at 20 ± 1°C over a period of 28 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.