Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 618-321-5 | CAS number: 899-79-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented study according to international accepted guidelines and GLP compliant.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-[(2E)-2-(6-Methoxy-3,4-dihydronaphthalen-1(2H)-ylidene)ethyl]-2-methylcyclopentane-1,3-dione
- EC Number:
- 618-321-5
- Cas Number:
- 899-79-6
- Molecular formula:
- C19H22O3
- IUPAC Name:
- 2-[(2E)-2-(6-Methoxy-3,4-dihydronaphthalen-1(2H)-ylidene)ethyl]-2-methylcyclopentane-1,3-dione
- Test material form:
- solid: crystalline
- Details on test material:
- Test item: Methyl-secodione
CAS number: 899-79-6
Molecular formula: C19H22O3
Batch number: L4B021N
Physical state: Solid, crystalline powder
Colour: Beige
Storage: At room temperíture, dry place
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The preparation of the test solutions was based on the preliminary non GLP range finding test.
Two stock solutions were prepared in the test. The first stock solution was prepared at the beginning of the test and the second fresh stock solution was prepared on the next day. For the stock solutions 100.0 mg of test item was taken into 1 000 ml Elendt M4 medium. The suspensions were stirred for 24 hours. After it the suspensions were filtered. The first 75 ml filtrates were disposed. The concentration of the filtrates (stock solutions) was determined by validated HPLC
method.
The measured concentrations of the stock solution I and II were 10.03 and 11.31 mg/L respectively.
In the case of both stock solutions seven dilution levels were prepared by mixing appropriate volume of Elendt M4 medium and stock solution of the test item. The maximum separation factor between of test vessels was 2.0.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test species: Daphnia magna Straus
Age: < 24 hours neonates
Origin: National Institute of Environmental Health
Culture conditions: The test species is maintained in Elendt M4 medium with culture conditions at 21±1 °C and a light/dark cycle of 16/8 hours with light intensities of less than 1000 Lux. The cultures are fed with centrifugalized green alga (Pseudokirchneriella subcapitata) cells daily at weekdays.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- The temperature of thermostat was recorded daily and registered continuously.
At the start of the test: 21.1 °C
After 24 hours: 21.1 °C
At the end of the test: 21.1 °C
The temperature inside the thermostat at the test vessels was measured and registered continuously. - pH:
- The pH was measured at each concentration level at the following time points:
- At the start (day 0, test solution I) from the rest test solution.
- At 24h (day 1/a, test solution I) from each test vessels.
- At 24h (day 1/b, test solution II) from the rest test solution.
- At 48h (day 2, test solution II) from each test vessels.
The measured mean values varied in the range of 7.88 - 8.01. - Dissolved oxygen:
- The oxygen concentration was measured at each concentration level at the
following time points:
- At the start (day 0, test solution I) from the rest test solution.
- At 24h (day 1/a, test solution I) from each test vessels.
- At 24h (day 1/b, test solution II) from the rest test solution.
- At 48h (day 2, test solution II) from each test vessels.
The measured mean values varied in the range of 5.87 - 6.14. - Nominal and measured concentrations:
- In the test the following nominal concentrations of test solutions were applied:
test solution I.: 10.03; 6.69; 5.02; 3.34; 2.01; 1.17; and 0.84 mg/L
test solution II.: 11.31; 7.54; 5.66; 3.77; 2.26; 1.32; and 0.94 mg/L.
The average measured concentrations:
8.24; 5.46; 4.08; 2.73; 1.65, 0.96 and 0.70 mg test item/L. - Details on test conditions:
- The test was maintained under semi-static conditions for a period of 48 hours.
At the start of the study each test vessels contained 50 ml of test solutions I and five young daphnids. In the test four replicates total of 20 animals at each test
concentration level were investigated. The test vessels were covered with plastic lids and were kept in thermostat at 21 ± 1 °C with a 16/8 hours light/dark cycle.
After 24 hours of the start of the test the living young daphnids were replaced into the freshly prepared test solutions II. The new test vessels were continued to incubate in a thermostat at 21 ± 1 °C with a 16/8 hours light/dark cycle for additional 24 hours. Each control vessels containing five young daphnids in Elendt M4 medium were prepared in four replicates and kept under the same semi-static conditions.
The number of immobilised and abnormally behaved daphnids was recorded after 24h. A daphnia is considered to be immobile if it is not able to swim within 15 seconds after a gentle agitation of the test vessel. The daphnids were not fed during the test. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.61 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.54 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The ECx concentrations were determined by the ToxRat® statistic software.
The NOEC and LOEC were also determined but the results were not conclusive.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to this Daphnia magna, Acute Immobilisation Test for Methyl-secodion (CAS No. 899-79-6) the results indicated that the test item is hazardous to the aquatic environment [GHS Acute aquatic hazard Category Acute II. – 48 hr EC50 (for Crustacea) > 1 mg/L but ≤ 10 mg/L].
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.