Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 227-615-9 | CAS number: 5908-81-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 - 29 May 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to the OECD 201 Guideline and complied with the principles of GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- (2006, Annex 5 corrected 28 July 2011)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Barium tartrate
- EC Number:
- 227-615-9
- EC Name:
- Barium tartrate
- Cas Number:
- 5908-81-6
- Molecular formula:
- C4H4O6Ba
- IUPAC Name:
- Barium L(+)-(2R,3R)-tartrate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): Barium tartrate
- Appearance: White powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of 4.8 mL for possible analysis were taken from all test concentrations and the control at t=0 h and t=72 h and stored in a freezer until analysis. In addition, the filter containing undissolved residue was retained for possible analysis.
At the end of the exposure period, the replicates with algae were pooled at each concentration before sampling.
On the day of analysis, the test samples were defrosted at room temperature. The samples were diluted in a 25:1 (v:v) ratio with HNO3 and analysed. If necessary, the samples were further diluted with 4% HNO3 in M2-medium to obtain concentrations within the calibration range.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Preparation of test solutions started with a loading rate of 100 mg/L applying a 2-day period of magnetic stirring followed by a filtration through a 0.45 µm membrane filter (RC55, Whatman). The obtained Water Soluble Fraction (WSF) was used as the highest concentration. The lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. The final test solutions were all clear and colourless.
After preparation, volumes of 50 mL were added to each replicate of the respective test concentration. Subsequently, 1 mL of an algal suspension was added to each replicate providing a cell density of 10^4 cells/mL.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: NIVA CHL 1
- Source (laboratory, culture collection): In-house laboratory culture.
- Age of inoculum (at test initiation):
- Method of cultivation: Algae stock cultures were started by inoculating growth medium with algal cells from a pure culture on agar. The suspensions were continuously aerated and exposed to light in a climate room at a temperature of 21-24°C. 3 days before the start of the test, cells from the algal stock culture were inoculated in culture medium at a cell density of 1 x 10^4 cells/mL. The pre-culture was maintained under the same conditions as used in the test. The cell density was measured immediately before use.
ACCLIMATION
- Culturing media and conditions (same as test or not): Pre-culture medium is same as test (M2)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- 24 mg CaCO3/L
- Test temperature:
- 21.9 -23.0 ºC
- pH:
- 7.9-8.4
- Dissolved oxygen:
- Not measured
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Test groups represented 1.0, 3.2, 10, 32 and 100% of the WSF prepared at a loading rate of 100 mg/L, respectively.
At the start of the test, the actual test concentrations were between 80 and 87% relative to the expected loading rates. The concentrations remained stable at 97 to 101% of initial during the 72-hour test period. The range tested based on actual exposure concentrations corresponded therefore with 1.0, 3.2, 10, 32 and 100 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL, all-glass, containing 50 mL of test solution
- Aeration: no
- Initial cells density: 1x 10^4 cells/mL
- Control end cells density: 125 x 10^4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes (M2)
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: continuously
- Light intensity and quality: TLD-lamps with a light intensity within the range of 78 to 83 µE.m-2.s-1.
EFFECT PARAMETERS MEASURED :
- Algal density at t = 0 , 24, 48 and 72h. The test solutions at 100% WSF after 72 hours in the combined range-finding/limit test contained particles. It was consequently decided that spectrophotometric measurement could be disturbed in the final test. Therefore algal density was determined by use of a microscope and a counting chamber throughout the final tests.
TEST CONCENTRATIONS
- combined limit/range-finding test with solutions containing 0.1, 1.0, 10 and 100% of a WSF prepared at a loading rate of 100 mg/L.
- definitive test with solutions containing 1.0, 3.2, 10, 32 and 100% of the WSF prepared at a loading rate of 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI 7.7 - 15 mg/L / The nominal concentrations were analytically confirmed (recovery > 80%)
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI 0.86 - 3.7 mg/L / The nominal concentrations were analytically confirmed (recovery > 80%)
- Details on results:
- Growth rates were in the range of the controls at the concentrations 1.0 and 3.2 mg/l during the 72-hour test period, whereas the growth rate of algae exposed to 10 mg/l and higher were increasingly reduced.
Statistically significant inhibition of growth rate was found at the lowest test concentration of 1.0 mg/l and at concentrations of 10 mg/l and higher. The NOEC was set at the in-between concentration of 3.2 mg/l despite the statistical significant reduction at the lower test group of 1.0 mg/l. Note further that the reduction in both groups was far below the biological relevant 10% level
Microscopic observations at the end of the test revealed a normal and healthy appearance of the exposed cells when compared to the control.
Acceptability of the test:
- In the controls, cell density increased by an average factor of >16 within 2 days (i.e. 69).
- The mean coefficient of variation for section-by-section specific growth rates in the control cultures did not exceed 35% (i.e. 35%).
- The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures did not exceed 7% (i.e. 2.6%). - Results with reference substance (positive control):
- The EC50 for growth rate inhibition (72h-ERC50) was 1.3 mg/L with a 95% confidence interval ranging from 1.1 to 1.5 mg/L. The historical ranges for growth rate inhibition lie between 0.82 and 2.3 mg/L. Hence, the 72h-ERC50 for the algal culture tested corresponds with this range.
Any other information on results incl. tables
Table 1 Percentage inhibition of growth rate (total test period) during the final test
Barium tartrate Concentration (mg/l) |
Mean |
Std. Dev. |
n |
%Inhibition |
Control |
1.411 |
0.0361 |
6 |
0.0 |
1.0 |
1.370 |
0.0111 |
3 |
2.9* |
3.2 |
1.362 |
0.0371 |
3 |
3.5# |
10 |
0.531 |
0.0099 |
3 |
62.4* |
32 |
0.427 |
0.0786 |
3 |
69.7* |
100 |
0.025 |
0.0429 |
3 |
98.2* |
* - effect was statistically significant
#- effect biologically insignificant (<10%)
Table 2 Percentage inhibition of growth rate at different time intervals during the final test
Barium tartrate Concentration (mg/l) |
n |
0 – 24 h |
24 – 48 h |
48 – 72h |
|||
Mean |
%Inhibition |
Mean |
%Inhibition |
Mean |
%Inhibition |
||
Control |
6 |
0.859 |
0.0 |
1.685 |
0.0 |
1.689 |
0.0 |
1.0 |
3 |
0.986 |
-14.8 |
1.397 |
17.1 |
1.728 |
-2.3 |
3.2 |
3 |
0.842 |
1.9 |
1.355 |
19.6 |
1.888 |
-11.8 |
10 |
3 |
0.833 |
2.9 |
0.254 |
84.9 |
0.505 |
70.1 |
32 |
3 |
0.846 |
1.5 |
-0.158 |
109.4 |
0.594 |
64.9 |
100 |
3 |
0.345 |
59.9 |
0.112 |
93.3 |
-0.382 |
122.6 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study with Pseudokirchneriella subcapitata, 72h-ErC50 was 11 mg/L and the 72h-ErC10 was 2.3 mg/L.
- Executive summary:
A 72-h algae growth inhibition test was performed with the substance according to OECD 201 and in compliance with GLP principles. Test groups represented 1.0, 3.2, 10, 32 and 100% of the WSF prepared at a loading rate of 100 mg/L, respectively. At the start of the test, the actual test concentrations were between 80 and 87% relative to the expected loading rates. The concentrations remained stable at 97 to 101% of initial during the 72-hour test period. The range tested based on actual exposure concentrations corresponded therefore with 1.0, 3.2, 10, 32 and 100 mg/L.
Under the conditions of the present study with Pseudokirchneriella subcapitata, 72h-ErC50 was 11 mg/L and the 72h-ErC10 was 2.3 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.