Ethanol

Additional information on environmental fate and behaviour

Administrative data

Endpoint:

additional information on environmental fate and behaviour

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

- Principle of test: To identify bacterial strains that can utilise ethanol as a source of carbon
- Short description of test conditions: Samples used as sources of bacteria collected from a number of sources and grown in media with 2-butoxyethanol. These were then plated on solid growth medium, incubated then colonies harvested and DNA sequenced using PCR technique.
- Parameters analysed / observed: strains that had grown under the prescribed conditions using gene sequencing.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Ethanol

EC Number:

200-578-6

EC Name:

Ethanol

Cas Number:

64-17-5

Molecular formula:

C2H6O

IUPAC Name:

ethanol

Test material form:

liquid

Results and discussion

Any other information on results incl. tables

The following strains were isolated and all found capable of utilising 2 -butoxyethanol as a carbon source:

Bacteria	Gram	Source (all in Germany)
Pseudomonas knackmussii	Gram-negative	Forest soil, Stuttgart
Pseudomonas putida	Gram-negative	Forest soil, Stuttgart
Hydrogenophaga pseudoflava	Gram-negative	Forest soil, Stuttgart
Pseudomonas umsongensis	Gram-negative	Forest soil, Stuttgart
Gordonia terrae	Gram-positive	Biotrickling filter, Stuttgart
Pseudomonas extremaustralis	Gram-negative	Biotrickling filter, Stuttgart
Pseudomonas plecoglossicida	Gram-negative	Activated sludge, Stuttgart
Pseudomonassp	Gram-negative	Activated sludge, Stuttgart
Pseudomonas putida	Gram-negative	Bioscrubber, Rastatt
Pseudomonas vancouverensis	Gram-negative	Bioscrubber, Rastatt
Cupriavidus oxalaticus	Gram-negative	Activated sludge, Stuttgart

Applicant's summary and conclusion

Executive summary:

In a study to examine how widely distributed are bacteria capable of utilising ethanol as a source of carbon, all 11 bacterial strains sourced from four different bacterial colonies (two biofilter/bioscrubbers that can be considered adapted sources) and forest soil and activated sludge that can be considered non-adapted) were found capable of degrading the substance. It can be concluded that the bacteria capable of degrading ethanol are widespread.