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Diss Factsheets
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EC number: 289-339-5 | CAS number: 87741-01-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented publication meeting basic scientific principles.
Data source
Reference
- Reference Type:
- publication
- Title:
- Improved method for mutagenicity testing of gaseous compounds by using a gas sampling bag.
- Author:
- Araki, A. et al.
- Year:
- 1 994
- Bibliographic source:
- Mutat Res. 307; 335-344.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- results only shown as graphical, not tabulated form, no data on cytotoxicity, no positive controls performed
- Principles of method if other than guideline:
- 1,3-butadiene was tested by an improved method for mutagenicity testing of gaseous compounds by using a gas sampling bag.
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Buta-1,3-diene
- EC Number:
- 203-450-8
- EC Name:
- Buta-1,3-diene
- Cas Number:
- 106-99-0
- Molecular formula:
- C4H6
- IUPAC Name:
- buta-1,3-diene
- Details on test material:
- - Name of test material (as cited in study report): 1,3-Butadiene
- Analytical purity: no data
- Physical state: gas
- Source: Tokyo Kasei Co. Ltd., Tokyo (Japan).
Constituent 1
Method
- Target gene:
- Genes involved in Histidine-synthesis
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Phenobarbital and 5,6-benzoflavone induced rat liver S9
- Test concentrations with justification for top dose:
- 0, 10, 20 and 50 %
- Vehicle / solvent:
- Air
- Details on test system and experimental conditions:
- - Exposure duration: 48 h
Bacterial plates were made by the agar overlay method, the amount of top agar was 2 ml per plate, the amount of S9 was 100 µl per plate, the exposure temperature was 37°C, the exposure period was 48 h and the exposure volume of gas was 500 ml per plate. - Statistics:
- No statistics performed
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not specified
- Additional information on results:
- 1,3-Butadiene is clearly mutagenic to TA1535 with metabolic activation at a concentration of more than 3% and shows a dose-related marginal
increase of revertant colonies on TA100 with S9 mix.
Any other information on results incl. tables
The results were not given in tabulated form.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive with metabolic activation
1,3-Butadiene is mutagenic in bacteria. - Executive summary:
Araki et al., 1994 developed a simple and safety gas exposure method for mutagenicity testing of gases.
1,3 -butadiene was tested at 0, 10, 20 and 50 % in air in different S. typhimurium strains (TA98, TA100, TA1535 and TA1537) and in E. coli WP2 uvrA, both with and without the addition of a rat liver homogenate metabolising system. Data on cytotoxicity were not shown.
1,3 -Butadiene was clearly mutagenic to TA1535 with metabolic activation at a concentration of more than 3% and showed a dose-related marginal increase of revertant colonies on TA100 with S9 mix.
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