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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
For details on endpoint specific justification please see read-across report in section 13 or find a link in cross-reference “assessment report”.
Reason / purpose for cross-reference:
assessment report
Reason / purpose for cross-reference:
read-across source
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
43.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 95% CI: 9.4-91.7
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
182 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 95% CI: 84.7-282
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
1 558 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 95% CI: 966-3760
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
26.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of heterotrophic respiration
Remarks:
respiration rate
Remarks on result:
other: 95% CI: 1.8-72.4
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
341 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of heterotrophic respiration
Remarks:
respiration rate
Remarks on result:
other: 95% CI: 137-1787
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
291 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of nitrification rate
Remarks on result:
other: 95% CI: 98.7-978
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
1 574 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of nitrification rate
Remarks on result:
other: 95% CI: 583-76327
Details on results:
Due to the very similar molecular weight of source and target substance, the effect concentrations for the source substance are also valid for the target substance without further adjustment.
Validity criteria fulfilled:
yes
Conclusions:
In an activated sludge respiration inhibition test according to OECD 209 (2010) with the source substance 1,6-hexane diamine (HMD; CAS No. 124-09-4), nitrification proved to be the most sensitive endpoint (highest test item concentration: 1000 mg/L):
EC50 (3h; total respiration): 1558 mg/L;
EC50 (3h; heterotrophic respiration): >> 1000 mg/L;
EC50 (3h; nitrification): 291 mg/L.
Because of the very similar molecular weight of source and target substance, these results are directly applicable to the target substance DCH.
Executive summary:

The study used as source investigated the STP microorganism toxicity according to OECD TG 209 (2010). The study results of the source compound were considered applicable to the target compound. Justification and applicability of the read-across approach (structural analogue) is outlined in the read-across report in section 13 or find a link in cross reference “assessment report”.

Description of key information

In an activated sludge respiration inhibition test according to OECD 209 (2010) with the source substance 1,6-hexane diamine (HMD; CAS No. 124-09-4), nitrification proved to be the most sensitive endpoint (highest test item concentration: 1000 mg/L):
EC50 (3h; total respiration): 1558 mg/L;
EC50 (3h; heterotrophic respiration): >> 1000 mg/L;
EC50 (3h; nitrification): 291 mg/L.
Because of the very similar molecular weight of source and target substance, these results are directly applicable to the target substance DCH.

Key value for chemical safety assessment

EC50 for microorganisms:
291 mg/L

Additional information

The study used as source investigated the STP microorganism toxicity according to OECD TG 209 (2010). The study results of the source compound were considered applicable to the target compound. Justification and applicability of the read-across approach (structural analogue) is outlined in the read-across report in section 13.