1,4-dichlorobenzene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP declaration given but no certificate

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dichlorobenzene

EC Number:

203-400-5

EC Name:

1,4-dichlorobenzene

Cas Number:

106-46-7

Molecular formula:

C6H4Cl2

IUPAC Name:

1,4-dichlorobenzene

Test material form:

other: solid

Details on test material:

p-dichlorobenzene, lot: 08146-P
content 99.9 %
Paradichlorobenzene was prepared as a 60% paste in destilled water and administered at a volume of 3.3 ml/kg
Stability and absorbtion of the test substance were not determined.

Test animals

Species:

rat

Strain:

other: CFY (Sprague-Dawley origin)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 7-10 weeks
- Weight at study initiation: 202-251 g
- Fasting period before study:
- Housing: individually
- Diet ad libitum
- Water ad libitum
- Acclimation period: 6 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-22
- Humidity (%): 54
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Type of coverage:

other: gauze which was held in place with an impermeable dressing encircled firmly around the trunk.

Vehicle:

water

Details on dermal exposure:

To the clipped back of rats the testsubstance was applied as paste and covered by gauze which was held in place by an impermeable dressing encircled firmly around the trunk for 24 hours.

Duration of exposure:

24 hours exposure period.

Doses:

single dermal dose of 2000 mg/kg bw

No. of animals per sex per dose:

5 male and 5 female rats

Control animals:

no

Details on study design:

To the clipped back of rats the testsubstance was applied as paste and covered by gauze which was held in place by an impermeable dressing encircled firmly around the trunk for 24 hours. Afterwards the dressing was removed and the treated area of the skin was washed with warm water and blotted dry. the observation period was 14 day. then all animal were sacrificed and macroscopically examined.

Statistics:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD0

Effect level:

> 2 000 mg/kg bw

Remarks on result:

other: no animal died

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Remarks on result:

other: no clinical signs of systemic reaction to treatment, no dermal changes or irritation responses, no animal died

Mortality:

no deaths following a single dermal dose of Paradichlorbenzene at 2,0 g/kg bodyweight.

Clinical signs:

other: no clinical signs of systemic reaction to treatment. no dermal changes or irritation responses.

Gross pathology:

Terminal autopsy findings were normal

Any other information on results incl. tables

	Bodyweight (g) at Day
Sex	1	8	15
Male	232	282	329
	250	316	396
	249	316	386
	251	316	380
	250	320	385
Female	206	240	284
	250	275	290
	202	232	255
	206	236	260
	250	286	325

Applicant's summary and conclusion

Executive summary:

In a study according OECD Guideline 402 (Acute Dermal Toxicity) and GLP acute dermal toxicity was tested by applying 2000 mg/kg bw as paste to the back of male and female rats for 24 hours. No clinical signs of systemic reaction to treatment and no dermal changes or irritation responses were observed. No animal died during the 14 day observation period.

The acute lethal dermal dose of paradichlorobenzene to rats was found to be greater than 2000 mg/kg bodyweight .